CAJ | 학술논문

目的为了探讨胃癌组织中端粒酶与细胞凋亡的关系。方法采用原位杂交和原位末端DNA片段标记技术对60例胃癌组织中端粒酶和细胞凋亡指数(AI)进行检测。结果 60例胃癌中49例端粒酶阳性(81.7%)，55例(91.7%)可观察到凋亡细胞。32例淋巴结转移癌中端粒酶阳性率为 93.8%，明显高于无淋巴结转移组的67.9%(P<0.05)；淋巴结转移组的AI为5.51±2.70，明显低于无淋巴结转移组的8.82±3.17(P<0.05)；淋巴结转移组胃癌中的端粒酶活性与AI呈负相关(P<0.05)。结论端粒酶活性和细胞凋亡可作为胃癌的生物学行为和预后的观察指标。
목적위료탐토위암조직중단립매여세포조망적관계。방법채용원위잡교화원위말단DNA편단표기기술대60례위암조직중단립매화세포조망지수(AI)진행검측。결과 60례위암중49례단립매양성(81.7%)，55례(91.7%)가관찰도조망세포。32례림파결전이암중단립매양성솔위 93.8%，명현고우무림파결전이조적67.9%(P<0.05)；림파결전이조적AI위5.51±2.70，명현저우무림파결전이조적8.82±3.17(P<0.05)；림파결전이조위암중적단립매활성여AI정부상관(P<0.05)。결론단립매활성화세포조망가작위위암적생물학행위화예후적관찰지표。
Objective　To investigate the relationship between the telomer ase activity and apoptosis in gastric cancer.Methods　In situ hy bridization and in situ end labeling of fragment DNA were used to detect the tel omerase activity and apoptosis in 60 cases gastric cancer.Results　Telomerase positivity was detected in 49(81.7%) and apoptosis cell was found i n 55(91.7%) of 60 cases gastric cancer.Telomeras positivity was significantly hi gh er with lymph node metastasis cancer(93.8%) than that without metastasis (6 7.9%,P<0.05).Apoptotic index(AL)was 5.51±2.70 in metastased cancer,it was 8.82 ± 3.17 in non-metastased one(P<0.05).Inverse correlation between telomerase activ ity and AI revealed in metastased cancer(P<0.05). Conclusions　The results suggest that telomeras activity and AI are associated with malignant biological behavior,they may be potential prognos tic factor in gastric cancer.