哈尔滨医科大学学报
哈爾濱醫科大學學報
합이빈의과대학학보
JOURNAL OF HARBIN MEDICAL UNIVERSITY
2009年
6期
527-531
,共5页
胰岛新生相关蛋白%空间稳定免疫脂质体%免疫活性%细胞靶向性
胰島新生相關蛋白%空間穩定免疫脂質體%免疫活性%細胞靶嚮性
이도신생상관단백%공간은정면역지질체%면역활성%세포파향성
islet neogenesis-associated protein%sterically stabilized immunoliposome%immunoreactivity%targeting
目的 以肝细胞生长因子为包被对象,研究胰岛新生相关蛋白(INGAP)空间稳定免疫脂质体的制备方法 、理化性质和体外靶向性.方法 制备含PDP-PEG-HSPE的空间稳定脂质体,共价连接马来酰亚胺衍生化IN-GAP.激光散射粒度仪测定其粒径分布,比色法测定包封率,透析法检测药物释放度及累积泄漏率,ELISA法检测脂质体表面的抗体免疫活性,流式细胞仪及免疫荧光考察该免疫脂质体对ARIP细胞(大鼠胰腺导管癌细胞系)的结合活性.结果 INGAP-SIL[HGF]脂质体的粒径分布为(95.86±6.54)nm;包封率为(88.37±3.58)%;24 h释放度为(23.26±2.45)%;4 ℃保存时7 d内药物累积泄漏率小于3%;4℃和25℃条件下保存7 d,免疫活性均在75%以上;INGAP连接到脂质体表面后,其免疫活性基本保留;空间稳定脂质体能特异性地结合ARIP细胞.结论 通过PDP-PEG-HSPE共价连接INGAP制备的INGAP-SIL理化性质较稳定,能基本保留INGAP的免疫活性,具有体外靶向细胞抗原的能力.
目的 以肝細胞生長因子為包被對象,研究胰島新生相關蛋白(INGAP)空間穩定免疫脂質體的製備方法 、理化性質和體外靶嚮性.方法 製備含PDP-PEG-HSPE的空間穩定脂質體,共價連接馬來酰亞胺衍生化IN-GAP.激光散射粒度儀測定其粒徑分佈,比色法測定包封率,透析法檢測藥物釋放度及纍積洩漏率,ELISA法檢測脂質體錶麵的抗體免疫活性,流式細胞儀及免疫熒光攷察該免疫脂質體對ARIP細胞(大鼠胰腺導管癌細胞繫)的結閤活性.結果 INGAP-SIL[HGF]脂質體的粒徑分佈為(95.86±6.54)nm;包封率為(88.37±3.58)%;24 h釋放度為(23.26±2.45)%;4 ℃保存時7 d內藥物纍積洩漏率小于3%;4℃和25℃條件下保存7 d,免疫活性均在75%以上;INGAP連接到脂質體錶麵後,其免疫活性基本保留;空間穩定脂質體能特異性地結閤ARIP細胞.結論 通過PDP-PEG-HSPE共價連接INGAP製備的INGAP-SIL理化性質較穩定,能基本保留INGAP的免疫活性,具有體外靶嚮細胞抗原的能力.
목적 이간세포생장인자위포피대상,연구이도신생상관단백(INGAP)공간은정면역지질체적제비방법 、이화성질화체외파향성.방법 제비함PDP-PEG-HSPE적공간은정지질체,공개련접마래선아알연생화IN-GAP.격광산사립도의측정기립경분포,비색법측정포봉솔,투석법검측약물석방도급루적설루솔,ELISA법검측지질체표면적항체면역활성,류식세포의급면역형광고찰해면역지질체대ARIP세포(대서이선도관암세포계)적결합활성.결과 INGAP-SIL[HGF]지질체적립경분포위(95.86±6.54)nm;포봉솔위(88.37±3.58)%;24 h석방도위(23.26±2.45)%;4 ℃보존시7 d내약물루적설루솔소우3%;4℃화25℃조건하보존7 d,면역활성균재75%이상;INGAP련접도지질체표면후,기면역활성기본보류;공간은정지질체능특이성지결합ARIP세포.결론 통과PDP-PEG-HSPE공개련접INGAP제비적INGAP-SIL이화성질교은정,능기본보류INGAP적면역활성,구유체외파향세포항원적능력.
Objective To study the preparation methods,physical and chemical Droperties of the isletneogenesis-associated protein(INGAP)sterically stabilized immunoliposomes.Methods An endgroupfunctionalized polyethylene glycol-lipid deirvative(pyridyhhiopropionoylamino-polyethylene glycol-hydr0-genated soy phosphatidylethanolamine,PDP-PEG-HSPE)was synthesized and incorporated tostericallv stabilized liposomes.After mild thiolysis of the PDP groups by dithiothreitol,liposomes were covalentlv linked with maleimide-derivatized INGAP and formed sterically stabilized immunoliDosomes.The size distribution,immunoreactivity of INGAP-modified sterically stabilized liposomes(INGAP-SIL[HGF])and specific binding properties of the INGAP-SIL[HGF]to ARIP cells were determined,separately.Results The INGAP-SIL[HGF]had a narrow size distribution after extrusion and the mean size of this immunoliposomes was(95.86±6.54)nm,encapsulation efficiency was(88.37±3.58)%.The immunoreac-tivity of INGAP can be preserved after efficient attachment of maleimide.derivatized INGAP to the surfaceof liposomes.But calculated per antibody concentration,the immunoreactivity of INGAPP-SIL[HGF]would obviously decrease compared to that of INGAP-SIL[HGF].Significantly higher binding of INGAP-SIL to fixed ARIP cells directed by INGAP was obtained compared to other preparations.Con-clusion INGAP-SIL[HGF]prepared by PDP-PEG-HSPE method maintains stabile physicolchemical properties and most immunoreactivity of INGAP and is able to bind nuclear antigens in vitro.
