CAJ | 학술논문

本试验研究建立了同时测定土壤中环丙氨嗪和三聚氰胺残留量的高效液相色谱法.红壤、潮土等5种土壤样品经氨水/甲醇 (5/95,v/v)超声提取3次,浓缩处理后上机检测.环丙氨嗪和三聚氰胺的标准曲线在0.1 ～ 15.0 μg/ml浓度范围内线性关系良好,绝对系数(R~2)分别为1.0000和0.9998;在0.5 ～ 5 mg/kg添加范围内,环丙氨嗪和三聚氰胺在土壤中的平均回收率分别为87.2% ～ 101.1% 和 75.3% ～ 101.6%,变异系数分别为3.3% ～ 8.1%、1.6% ～ 9.9%,最低检测限分别为0.05 mg/kg、0.07 mg/kg.与国际上气相/液相色谱-质谱连用法相比,操作简单,经济方便易于普及.
본시험연구건립료동시측정토양중배병안진화삼취청알잔류량적고효액상색보법.홍양、조토등5충토양양품경안수/갑순 (5/95,v/v)초성제취3차,농축처리후상궤검측.배병안진화삼취청알적표준곡선재0.1 ～ 15.0 μg/ml농도범위내선성관계량호,절대계수(R~2)분별위1.0000화0.9998;재0.5 ～ 5 mg/kg첨가범위내,배병안진화삼취청알재토양중적평균회수솔분별위87.2% ～ 101.1% 화 75.3% ～ 101.6%,변이계수분별위3.3% ～ 8.1%、1.6% ～ 9.9%,최저검측한분별위0.05 mg/kg、0.07 mg/kg.여국제상기상/액상색보-질보련용법상비,조작간단,경제방편역우보급.
An analytic method with HPLC was developed to determine the residues of cyromazine and melamine in soils. Soil samples were extracted thrice with ammonia/methanol (5/95,v/v) by ultrasonic and concentrated, and then analyzed by HPLC. The calibration curves for cyromazine and melamine were linear in concentration range of 0.1-15.0 μg/ml (R~2=1.0000 and 0.9998, respectively) . In the concentration of 0.5-5 mg/kg, the average recoveries of cyromazine and melamine were 87.2% - 101.1% and 75.3% - 101.6% with the variation coefficient of 3.3% - 8.1% and 1.6% - 9.9% respectively. The limit of detection was 0.05 mg/kg and 0.07 mg/kg, respectively.