北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2009年
12期
810-814
,共5页
岳利峰%陈家旭%王大伟%霍素坤%岳广欣%梁媛%李晶晶
嶽利峰%陳傢旭%王大偉%霍素坤%嶽廣訢%樑媛%李晶晶
악리봉%진가욱%왕대위%곽소곤%악엄흔%량원%리정정
逍遥散%肝郁脾虚证%基因%调节机制%AMPA受体%大鼠
逍遙散%肝鬱脾虛證%基因%調節機製%AMPA受體%大鼠
소요산%간욱비허증%기인%조절궤제%AMPA수체%대서
Xiaoyao Powder%liver-stagnation and spleen-deficiency syndrome%gene%regulating mechanism%AMPA receptor%rats
目的 探讨逍遥散治疗肝郁脾虚证的基因调节机制.方法 75只SD大鼠随机等分为5组,正常组、模型组、假手术组、6-氰基-7-nitroquinoxaline-2,3-二酮(CNQX组)和逍遥散组.以21 d慢性束缚造模型组,在此基础上,运用脑立体定位仪微量注射α-氨基羟甲基恶唑丙酸(AMPA)受体的拮抗剂造CNQX组,加造假手术组为了评估手术创伤对模型的影响程度.逍遥散组造模方法和CNQX组尽可能相似,突出逍遥散和CNQX二者干预的可比性.运用RT-PCR一步法检测海马CA1、CA3和杏仁核AMPA受体的2个重要亚基GluR1 mRNA、GluR2 mRNA的表达变化,比较CNQX组和逍遥散组在各区指标变化趋势是否一致.结果 在海马CA1和CA3区,正常组、CNQX组和逍遥散组间GluR1 mRNA和GluR2 mRNA表达均无统计学意义(P>0.05);在基底外侧杏仁核BLA区,CNQX组由于拮抗AMPA受体,GIuR1 mRNA和GluR2 mRNA均表达最低,但正常组和逍遥散组间均无统计学意义(P>0.05).排除了手术创伤等混杂因子,逍遥散组和CNQX组上述2个指标RT-PCR表达结果在海马和杏仁核变化趋势均相似.结论 逍遥散对肝郁脾虚证模型大鼠海马和杏仁核AMPA受体亚基基因表达的调节机制可能和CNQX的调节机制吻合.CNQX通过拮抗杏仁核AMPA受体,抑制杏仁核兴奋,缓解海马各区受损.进而推断逍遥散通过纠正杏仁核和海马的"兴奋一抑制"失衡,重建稳态,来治疗肝郁脾虚证.
目的 探討逍遙散治療肝鬱脾虛證的基因調節機製.方法 75隻SD大鼠隨機等分為5組,正常組、模型組、假手術組、6-氰基-7-nitroquinoxaline-2,3-二酮(CNQX組)和逍遙散組.以21 d慢性束縳造模型組,在此基礎上,運用腦立體定位儀微量註射α-氨基羥甲基噁唑丙痠(AMPA)受體的拮抗劑造CNQX組,加造假手術組為瞭評估手術創傷對模型的影響程度.逍遙散組造模方法和CNQX組儘可能相似,突齣逍遙散和CNQX二者榦預的可比性.運用RT-PCR一步法檢測海馬CA1、CA3和杏仁覈AMPA受體的2箇重要亞基GluR1 mRNA、GluR2 mRNA的錶達變化,比較CNQX組和逍遙散組在各區指標變化趨勢是否一緻.結果 在海馬CA1和CA3區,正常組、CNQX組和逍遙散組間GluR1 mRNA和GluR2 mRNA錶達均無統計學意義(P>0.05);在基底外側杏仁覈BLA區,CNQX組由于拮抗AMPA受體,GIuR1 mRNA和GluR2 mRNA均錶達最低,但正常組和逍遙散組間均無統計學意義(P>0.05).排除瞭手術創傷等混雜因子,逍遙散組和CNQX組上述2箇指標RT-PCR錶達結果在海馬和杏仁覈變化趨勢均相似.結論 逍遙散對肝鬱脾虛證模型大鼠海馬和杏仁覈AMPA受體亞基基因錶達的調節機製可能和CNQX的調節機製吻閤.CNQX通過拮抗杏仁覈AMPA受體,抑製杏仁覈興奮,緩解海馬各區受損.進而推斷逍遙散通過糾正杏仁覈和海馬的"興奮一抑製"失衡,重建穩態,來治療肝鬱脾虛證.
목적 탐토소요산치료간욱비허증적기인조절궤제.방법 75지SD대서수궤등분위5조,정상조、모형조、가수술조、6-청기-7-nitroquinoxaline-2,3-이동(CNQX조)화소요산조.이21 d만성속박조모형조,재차기출상,운용뇌입체정위의미량주사α-안기간갑기악서병산(AMPA)수체적길항제조CNQX조,가조가수술조위료평고수술창상대모형적영향정도.소요산조조모방법화CNQX조진가능상사,돌출소요산화CNQX이자간예적가비성.운용RT-PCR일보법검측해마CA1、CA3화행인핵AMPA수체적2개중요아기GluR1 mRNA、GluR2 mRNA적표체변화,비교CNQX조화소요산조재각구지표변화추세시부일치.결과 재해마CA1화CA3구,정상조、CNQX조화소요산조간GluR1 mRNA화GluR2 mRNA표체균무통계학의의(P>0.05);재기저외측행인핵BLA구,CNQX조유우길항AMPA수체,GIuR1 mRNA화GluR2 mRNA균표체최저,단정상조화소요산조간균무통계학의의(P>0.05).배제료수술창상등혼잡인자,소요산조화CNQX조상술2개지표RT-PCR표체결과재해마화행인핵변화추세균상사.결론 소요산대간욱비허증모형대서해마화행인핵AMPA수체아기기인표체적조절궤제가능화CNQX적조절궤제문합.CNQX통과길항행인핵AMPA수체,억제행인핵흥강,완해해마각구수손.진이추단소요산통과규정행인핵화해마적"흥강일억제"실형,중건은태,래치료간욱비허증.
Objective To investigate the mechanism of gene regulation of xiaoyao Powder in the treatment of liver-stagnation and spleen-deficiency syndrome.Methods All SD rats(n=75)were randomly divided into 5 groups,including the normal group,model group,sham-operation group,CNQX group and Xiaoyao Powder group.The model was established by chronic restraint for 21 days and then CNQX group wag given CNQX microinjection by cerebral stereotaxis instrument.The sham-operation group was set up for review the influence of operative injury on the model.The modeling method of xiaoyao Powder group was as the same ag possible with that of CNQX group for the comparison between these two groups. The expression changes of GluR1-mRNA and GluR2-mRNA were detected, which were two important subunits in CA1 and CA3 regions of hippocampus and AMPA receptor of amygdala, by onestep RT-PCR method. The trends of indexes in all regions were compared between CNQX group and Xiaoyao Powder group. Results The expressions of GluR1-mRNA and GluB2-mRNA had no statistical significance among the normal group, CNQX group and Xiaoyao Powder group in CA1 and CA3 regions of hippocampus (all P>0.05). In BLA region of amygdala the expressions of GluR1-mRNA and GluR2mRNA reached the lowest in CNQX group because the antagonism of CNQX to AMPA receptor, but had no statistical significance between the normal group and Xiaoyao Powder group ( all P>0. 05 ). The trends of the expressions of GluRI-mRNA and GluR2-mRNA in hippocampns and amygdala were similar showed by RT-PCR in CNQX group and Xiaoyao Powder group after dismissing confounding factors like operative injury. Conclusion The regulating mechanism of Xiaoyao Powder to the expressions of subunits of hippocampus and amygdala AMPA receptor may be coincidence with that of CNQX in rat model of liver-stagnation and spleen-deficiency syndrome. It is deduced that Xiaoyao Powder cures liver-stagnation and spleen-deficiency syndrome through correcting the disorder of "excitation-inhibition" of hippocampus and amygdala and reconstructing the homeostasis.