CAJ | 학술논문

基于血红蛋白、肌红蛋白等色素在近红外波段的吸收谱,采用近红外光谱(NIRS)技术,通过检测出射光相对入射光的衰减,可解算牛物组织中上述色素的浓度.基于稳态窄间分辨的NIRS研发了可检测组织中色素浓度的装置,该装置的传感器包括一个可发射3个不同波长的LED光源,以及与IED位于同一直线上且距离分别为30和40mm的2个检测器.用该装置检测了2块猪肉组织(分别为新鲜的猪背长肌和股直肌)的色素浓度,结果表明,背长肌和股直肌中血红蛋白、肌红蛋白等色素的总浓度分别为(6.42±1.51)μmol · ~(-1)和(15.48±4.54)μmol · ~(-1),接近于现有报道的经验值.在无损、实时、直接、简便检测猪肉组织的色素浓度方面,上述NIRS方法及装置是可信的.
기우혈홍단백、기홍단백등색소재근홍외파단적흡수보,채용근홍외광보(NIRS)기술,통과검측출사광상대입사광적쇠감,가해산우물조직중상술색소적농도.기우은태착간분변적NIRS연발료가검측조직중색소농도적장치,해장치적전감기포괄일개가발사3개불동파장적LED광원,이급여IED위우동일직선상차거리분별위30화40mm적2개검측기.용해장치검측료2괴저육조직(분별위신선적저배장기화고직기)적색소농도,결과표명,배장기화고직기중혈홍단백、기홍단백등색소적총농도분별위(6.42±1.51)μmol · ~(-1)화(15.48±4.54)μmol · ~(-1),접근우현유보도적경험치.재무손、실시、직접、간편검측저육조직적색소농도방면,상술NIRS방법급장치시가신적.
Based on the absorption spectra of hemoglobin and myoglobin in the near infrared band, the concentrations of these pigments in the biological tissues can be obtained using near infrared spectroscopy (NIRS) by detecting the intensity attenuation of the emitted light compared with the incident light. Based on the steady-state spatially resolved NIRS, the prototype for detecting the concentrations of tissue hemoglobin and myoglobin was independently developed by our group. The probe consisted of an LED light source which could emit three different wavelengths in the near infrared band, and two detectors which were placed on the same line with and at the distances of 30/40 mm to the LED. The pigment concentrations of two pieces of pork, one from the erector spinae and the other from the rectus femoris, were detected using this prototype. The total concentrations of hemoglobin and myoglobin (c_(total)) were (6. 42±1. 51)μmol · L~(-1) in the erector spinae, and (15. 48±4. 54)μmol · L~(-1) in the rectus femoris, respectively. The myoglobin was dominant in both of the results. These were consistent with the recent empirical reports. In summary, the NIRS method and prototype are authentic in detecting the pigment concentrations of pork tissue non-invasively, real-time, directly and conveniently.