CAJ | 학술논문

本研究对毛竹(Phyllostachys pubescens)根部(包括根际、根面及内生细菌)解磷细菌进行了筛选,并通过16S rDNA序列分析对其多样性进行了初步研究.从179株毛竹根部细菌中筛选出26株解磷细菌,包括14株根际细菌、8株根面细菌、4株内生细菌.其中,芽孢杆菌BK24的解磷活性最高,其解磷活性达到369.06 mg/L.16S rDNA序列分析表明:26株解磷细菌中,13株属于厚壁菌门(Firmicutes,50.00%),5株属于变形菌门β亚群(Betaproteobacteria,19.23%),4株属于变形菌门γ亚群(Gammaproteobacteria,15.38%),4株属于放线菌门(Actinobacteria,15.38%).芽孢杆菌属(Bacillus,38.46%)和伯克霍尔德氏菌属(Burkholderia,19.23%)为优势菌属.
본연구대모죽(Phyllostachys pubescens)근부(포괄근제、근면급내생세균)해린세균진행료사선,병통과16S rDNA서렬분석대기다양성진행료초보연구.종179주모죽근부세균중사선출26주해린세균,포괄14주근제세균、8주근면세균、4주내생세균.기중,아포간균BK24적해린활성최고,기해린활성체도369.06 mg/L.16S rDNA서렬분석표명:26주해린세균중,13주속우후벽균문(Firmicutes,50.00%),5주속우변형균문β아군(Betaproteobacteria,19.23%),4주속우변형균문γ아군(Gammaproteobacteria,15.38%),4주속우방선균문(Actinobacteria,15.38%).아포간균속(Bacillus,38.46%)화백극곽이덕씨균속(Burkholderia,19.23%)위우세균속.
26 phosphate-solubilizing bacteria were screened from 179 rhizosphere,rhizoplane and root endophytic bacterial strains of moso bamboo (Phyllostachys pubescens) plants. The phosphate-solubilizing bacteria included fourteen rhizobacteria isolates,eight rhizoplane bacteria isolates,and four endophytic bacteria isolates. Bacillus sp. BK24 showed the highest phosphate solubilizing activity (369.06 mg/L). Phylogenetic analysis based on the partial 16S rDNA sequences indicated that the strains were clustered into four groups:thirteen strains of Firmicutes (50.00%),five strains of Betaproteobacteria (19.23%),four strains of Gammaproteobacteria (15.38%) and four strains of Actinobacteria (15.38%). The dominant phosphate-solubilizing bacteria were Bacillus and Burkholderia,which was 38.46% and 19.23% respectively.