CAJ | 학술논문

目的:利用功能磁共振成像技术(fMRI),分别在施加色氨酸耗竭氨基酸混合物(TRP-)和配平氨基酸混合物(BAL)前后,对健康志愿者施加痛性电刺激,观察健康志愿者应用TRP-或BAL前后SⅠ区及SⅡ区的激活情况差异,探讨脑内5-羟色胺(5-HT)在SⅠ及SⅡ区中的分布情况及其在痛觉调控中的作用. 方法:实验采用两阶段交叉设计.TRP-和BAL配方参照经典的Young氏方法.右利手健康志愿者6名,分别于服用TRP-和BAL前后施加1倍痛阈(服药前痛阈)电刺激,同期进行全脑fMRI扫描,应用功能性神经图像分析(AFNI)软件包进行后处理,以显示服用TRP-或BAL前后SⅠ及SⅡ区的激活差异.使用SPSS 14.0统计软件对结果进行统计分析. 结果:(1)服用TRP-前后,双侧SⅠ区、SⅡ区均出现激活,其激活体积在服用TRP-前后具有明显差异(P＜0.05),服用TRP-后,上述脑区激活体积增大; (2)服用BAL前后,上述脑区激活体积未见明显差异(P>0.05);(3)服用TRP-前后,志愿者1倍痛阈具有明显差异(P＜0.01),服用TRP-后,志愿者痛阈明显降低.服用BAL前后,志愿者1倍痛阈未见明显差异(P＞0.05). 结论:在中枢神经系统中,5-HT起着重要的镇痛作用.服用TRP-后引起急性色氨酸耗竭(ATD),可以降低脑内5-HT水平,引起志愿者痛阈降低.应用TRP-前后,SⅠ区,SⅡ区激活体积具有明显差异(P＜0.05),提示这些脑区不仅参与痛觉调控,而且可能直接参与5-HT对痛觉的镇痛作用.
목적:이용공능자공진성상기술(fMRI),분별재시가색안산모갈안기산혼합물(TRP-)화배평안기산혼합물(BAL)전후,대건강지원자시가통성전자격,관찰건강지원자응용TRP-혹BAL전후SⅠ구급SⅡ구적격활정황차이,탐토뇌내5-간색알(5-HT)재SⅠ급SⅡ구중적분포정황급기재통각조공중적작용. 방법:실험채용량계단교차설계.TRP-화BAL배방삼조경전적Young씨방법.우리수건강지원자6명,분별우복용TRP-화BAL전후시가1배통역(복약전통역)전자격,동기진행전뇌fMRI소묘,응용공능성신경도상분석(AFNI)연건포진행후처리,이현시복용TRP-혹BAL전후SⅠ급SⅡ구적격활차이.사용SPSS 14.0통계연건대결과진행통계분석. 결과:(1)복용TRP-전후,쌍측SⅠ구、SⅡ구균출현격활,기격활체적재복용TRP-전후구유명현차이(P＜0.05),복용TRP-후,상술뇌구격활체적증대; (2)복용BAL전후,상술뇌구격활체적미견명현차이(P>0.05);(3)복용TRP-전후,지원자1배통역구유명현차이(P＜0.01),복용TRP-후,지원자통역명현강저.복용BAL전후,지원자1배통역미견명현차이(P＞0.05). 결론:재중추신경계통중,5-HT기착중요적진통작용.복용TRP-후인기급성색안산모갈(ATD),가이강저뇌내5-HT수평,인기지원자통역강저.응용TRP-전후,SⅠ구,SⅡ구격활체적구유명현차이(P＜0.05),제시저사뇌구불부삼여통각조공,이차가능직접삼여5-HT대통각적진통작용.
Objective To observe the different cerebral activations between pre-administration and post-administration of tryptophan-depleted amino acid mixture (TRP-)/balanced amino acid mixture (BAL) in healthy volunteers through functional magnetic resonance imaging (fMRI) when they received electrical stimulation at the same time. Furthermore, the effects of 5-hydroxytryptamine (5-HT) were explored in pain modulation network. Methods Two-phase crossover design was adopted. Six right-handed healthy volunteers participated in the experiment. TRP- and BAL were administered to each participant according to the Young's method. Volunteers were scanned under fMRI with or without administration of TRP- or BAL when receiving electrical stimulation simultaneously. Analysis of functional neuroimages (AFNI) software was used to show the different activations in the human brain between pre- and post-administration of TRP-/BAL. Then all the results were analyzed by SPSS 14.0. Results (1) Bilateral primary somatosensory cortex (SⅠ) and secondary somatosensory cortex (SⅡ) were activated during pre-administration and post-administration of TRP-, but the activated volumes of bilateral SⅠ and SⅡ during post-administration of TRP- were found significantly larger than that during pre-administration of TRP (P＜0.05). (2) All the activated regions showed no difference between pre- and post-admini-stration of BAL. (3) There were significant differences of pain threshold between pre- and post-admini-stration of TRP- (P＜0.01), which meant that pain threshold reduced remarkably after TRP- administration, while it showed no difference between pre- and post-administration of BAL (P＞0.05). Conclusion 5-HT plays an important role in pain modulation in the central nervous system, TRP- causes acute tryptophan depletion (ATD) through reducing the level of 5-HT in the human brain, which can induce the decrease of volunteers' pain threshold. The activated volumes of bilateral SⅠ and SⅡ display signi-ficant difference between pre- and post-administration of TRP-, suggesting these brain areas may not only involve in the 5-HT related activities but in pain modulation network as well.