CAJ | 학술논문

目的:研究香菇多糖醇沉规律及产物分子质量分布情况.方法:采用分步醇沉和分级醇沉方法研究香菇多糖醇沉工艺,用凝胶色谱法(GPC)表征香菇多糖分子质量的分布.结果:不同醇沉时间获得的香菇多糖质量相差不大,醇沉速度随时间延长逐渐降低.分步醇沉在乙醇体积分数为30%条件下,所得到的香菇多糖的质量最大.分级醇沉所得到的香菇多糖的质最随着乙醇体积分数的升高而增加,高体积分数乙醇沉淀所得的香菇多糖的质量差别不明显.乙醇体积分数为30%、50%和80%所得到的香菇多糖,其峰位分子质量(M_p)分别为22671、18694、6011D.结论:醇沉体积分数和方式对香菇多糖得率和分子质量分布均有较大影响.
목적:연구향고다당순침규률급산물분자질량분포정황.방법:채용분보순침화분급순침방법연구향고다당순침공예,용응효색보법(GPC)표정향고다당분자질량적분포.결과:불동순침시간획득적향고다당질량상차불대,순침속도수시간연장축점강저.분보순침재을순체적분수위30%조건하,소득도적향고다당적질량최대.분급순침소득도적향고다당적질최수착을순체적분수적승고이증가,고체적분수을순침정소득적향고다당적질량차별불명현.을순체적분수위30%、50%화80%소득도적향고다당,기봉위분자질량(M_p)분별위22671、18694、6011D.결론:순침체적분수화방식대향고다당득솔화분자질량분포균유교대영향.
A commercial sample of lentinans was refined and subjected to ethanol fractionations in two different ways:way No.1,use of ethanol concentration gradients of 10%,20%,30% to 90%,respectively,and way No.2,adjustment of ethanol concentration in supernatant to 10%,20%,30% to 90% to understand molecular weight distribution by gel filtration chromatography.Results showed that precipitation time had no notable effect on the precipitation mass of lentinans and precipitation rate exhibited a gradual decrease with prolonged precipitation time.A maximum precipitation mass of lentinans was obtained at 30% ethanol concentration in way No.2 precipitation.With increasing ethanol concentration,the precipitation mass of lentinans progressively increased in way No.1 precipitation and then trended nearly stable after reaching a high level.The peak molecular weight(M_p)of lentinans obtained at the ethanol concentrations of 30%,50% and 80% were 22671,18649 D and 6011D,respectively.In summary,both ethanol concentration and precipitation way had significantly effects on the precipitation mass and molecular weight distribution of lentinans