CAJ | 학술논문

为改良拮抗姜瘟病菌的芽孢杆菌,从未发病土壤分离得到1株对茄科雷尔氏菌(Ralstonia solanacearum)等8种病原菌具有较强拮抗能力的蜡状芽孢杆菌菌株L_1.为进一步改进该菌的拮抗活性,采用紫外线(UV)、甲基磺酸乙酯(EMS)、吖啶橙等诱变因素对L_1菌株进行诱变改良.结果表明:改良后菌株的拮抗性能显著提高,其中菌株EMS-2和UL_1的拮抗蛋白浓度分别比L_1菌株提高30.38%和29.18%,其差异均达极显著水平;L_1对UV照射十分敏感,尤其在前60 s,而90 s的照射处理正突变率最高,达24.8%;吖啶橙处理L_1后,没有筛选到发生正突变的菌株;L_1在EMS处理80 min后,致死率达到90%左右,因此,为大量获取正突变菌株,应采用较低剂量的EMS(0.16 mol·mL~(-1))、长时间处理程序(60 min为宜).
위개량길항강온병균적아포간균,종미발병토양분리득도1주대가과뢰이씨균(Ralstonia solanacearum)등8충병원균구유교강길항능력적사상아포간균균주L_1.위진일보개진해균적길항활성,채용자외선(UV)、갑기광산을지(EMS)、아정등등유변인소대L_1균주진행유변개량.결과표명:개량후균주적길항성능현저제고,기중균주EMS-2화UL_1적길항단백농도분별비L_1균주제고30.38%화29.18%,기차이균체겁현저수평;L_1대UV조사십분민감,우기재전60 s,이90 s적조사처리정돌변솔최고,체24.8%;아정등처리L_1후,몰유사선도발생정돌변적균주;L_1재EMS처리80 min후,치사솔체도90%좌우,인차,위대량획취정돌변균주,응채용교저제량적EMS(0.16 mol·mL~(-1))、장시간처리정서(60 min위의).
To improve the resistance of Bacillus cereus to Ralstonia solanacearum, an antagonistic bacterium belonging to B. cereus, named as L_1, was isolated from the ginger-growing soil, which was resistance to R. solanacearum at some degree. Ultraviolet rays (UV), ethyl methane sulfonate (EMS) and acridine orange (AO) were used as mutagens to treat strain L_1, and then the mutants were identified and screened. The results showed that the antagonistic abilities of mutant strains were significantly enhanced compared with the original strain L_1, and the concentrations of antagonistic protein of mutant-strains EMS-2 and UL_1, were 30.38% and 29.18% higher than that of original strain L_1 separately, being significant difference between the two mutant-strains and L_1. In addition, strain L_1 was sensitive to UV especially during the period of 60 s, and up to 90 s, the utmost positive mutation rate was detected (24.8%). However, no positive mutants were founded when the strain L_1 was treated with AO. Moreover, the lethal rate of strain L_1 was up to 90% with the treatment of EMS beyond 80 min,indicating that to obtain the more number of positive mutants, the lower dosage of EMS (0.16 mol·mL~(-1)) for longer treatment time (60 min) was the possible method of mutant treatment.