中华胰腺病杂志
中華胰腺病雜誌
중화이선병잡지
CHINESE JOURNAL OF PANCREATOLOGY
2010年
6期
412-414
,共3页
边洋%塔娜%李林%郑峤%张顺民%郑建明%左长京
邊洋%塔娜%李林%鄭嶠%張順民%鄭建明%左長京
변양%탑나%리림%정교%장순민%정건명%좌장경
胰腺炎,慢性%趋化因子类%单核细胞趋化蛋白-1%星形细胞%纤维化
胰腺炎,慢性%趨化因子類%單覈細胞趨化蛋白-1%星形細胞%纖維化
이선염,만성%추화인자류%단핵세포추화단백-1%성형세포%섬유화
Pancreatitis,chronic%Chemotactic factors%Monocyte chemoattractant protein-1%Astrocytes%Fibrosis
目的 检测慢性阻塞性胰腺炎小型猪血单核细胞趋化蛋白-1(MCP-1)含量及胰腺组织MCP-1蛋白表达,探讨其在胰腺纤维化中的作用.方法 采用不全结扎主胰管方法建立小型猪慢性阻塞性胰腺炎模型,术后4、6、8 周分批处死动物.观察胰腺病理变化;胶原(VG)染色评估胰腺纤维化程度;ELISA法检测血MCP-1浓度;免疫组化法检测胰腺组织MCP-1、α-SMA、PDGF、TGF-β1和NF-κB表达.结果 造模成功14头(58.3%).术后4周起胰腺体尾部呈萎缩性改变,间质纤维组织增生,炎细胞浸润,第8周改变最明显.胰腺纤维化Ⅰ期5头(35.7%),Ⅱ期4头(28.6%),Ⅲ期5头(35.7%).术后4、6、8周造模成功组血MCP-1含量分别为(102.44±36.25)pg/m1、(97.84±28.67)pg/ml、(94.32±28.42)pg/ml,显著高于对照组的(10.42±5.86)pg/ml、(8.58±4.86)pg/ml、(8.22±4.58)pg/ml(P值均<0.01).对照组胰腺无MCP-1蛋白表达,造模成功组胰腺组织内见MCP-1蛋白表达,且MCP-1表达与α-SMA、PDGF、TGF-β1和NF-κB表达呈正相关.结论 MCP-1在慢性阻塞性胰腺炎胰腺纤维化过程中起重要作用.
目的 檢測慢性阻塞性胰腺炎小型豬血單覈細胞趨化蛋白-1(MCP-1)含量及胰腺組織MCP-1蛋白錶達,探討其在胰腺纖維化中的作用.方法 採用不全結扎主胰管方法建立小型豬慢性阻塞性胰腺炎模型,術後4、6、8 週分批處死動物.觀察胰腺病理變化;膠原(VG)染色評估胰腺纖維化程度;ELISA法檢測血MCP-1濃度;免疫組化法檢測胰腺組織MCP-1、α-SMA、PDGF、TGF-β1和NF-κB錶達.結果 造模成功14頭(58.3%).術後4週起胰腺體尾部呈萎縮性改變,間質纖維組織增生,炎細胞浸潤,第8週改變最明顯.胰腺纖維化Ⅰ期5頭(35.7%),Ⅱ期4頭(28.6%),Ⅲ期5頭(35.7%).術後4、6、8週造模成功組血MCP-1含量分彆為(102.44±36.25)pg/m1、(97.84±28.67)pg/ml、(94.32±28.42)pg/ml,顯著高于對照組的(10.42±5.86)pg/ml、(8.58±4.86)pg/ml、(8.22±4.58)pg/ml(P值均<0.01).對照組胰腺無MCP-1蛋白錶達,造模成功組胰腺組織內見MCP-1蛋白錶達,且MCP-1錶達與α-SMA、PDGF、TGF-β1和NF-κB錶達呈正相關.結論 MCP-1在慢性阻塞性胰腺炎胰腺纖維化過程中起重要作用.
목적 검측만성조새성이선염소형저혈단핵세포추화단백-1(MCP-1)함량급이선조직MCP-1단백표체,탐토기재이선섬유화중적작용.방법 채용불전결찰주이관방법건립소형저만성조새성이선염모형,술후4、6、8 주분비처사동물.관찰이선병리변화;효원(VG)염색평고이선섬유화정도;ELISA법검측혈MCP-1농도;면역조화법검측이선조직MCP-1、α-SMA、PDGF、TGF-β1화NF-κB표체.결과 조모성공14두(58.3%).술후4주기이선체미부정위축성개변,간질섬유조직증생,염세포침윤,제8주개변최명현.이선섬유화Ⅰ기5두(35.7%),Ⅱ기4두(28.6%),Ⅲ기5두(35.7%).술후4、6、8주조모성공조혈MCP-1함량분별위(102.44±36.25)pg/m1、(97.84±28.67)pg/ml、(94.32±28.42)pg/ml,현저고우대조조적(10.42±5.86)pg/ml、(8.58±4.86)pg/ml、(8.22±4.58)pg/ml(P치균<0.01).대조조이선무MCP-1단백표체,조모성공조이선조직내견MCP-1단백표체,차MCP-1표체여α-SMA、PDGF、TGF-β1화NF-κB표체정정상관.결론 MCP-1재만성조새성이선염이선섬유화과정중기중요작용.
Objective To determine the serum concentration of MCP-1 and the expression of MCP-1 protein in the pancreas in the piglet with chronic obstructive pancreatitis and to explore the role of MCP-1 protein in pancreatic fibrosisits.Methods The piglet model of chronic obstructive pancreatitis was established by incomplete ligation of the pancreatic duct.The piglets were sacrificed at 4, 6, 8 weeks after induction.Pathological changes of pancreas were examined.Pancreatic fibrosis was assessed by VG staining.Serum MCP-1 concentrations were detected by ELISA method.MCP-1 and α-SMA, PDGF, TGF-β1 and NF-κB protein expression were detected by immunohistochemistry.Results The induction was successful in 14 piglets ( 58.3% ).Mild atrophic changes, interstitial fibrosis, chronic inflammatory cell infiltration could be observed in the body and tail of pancreas from the 4th week in the experimental group.The most obvious changes occurred in the 8th week.Stage Ⅰ pancreatic fibrosis occurred in 5 piglets (35.7%), stage Ⅱ in 4 piglets (28.6%), stage Ⅲ in 5 rats ( 35.7% ).Seurm MCP-1 at 4, 6, 8 weeks was ( 102.44 ± 36.25 ) pg/ml,(97.84 ± 28.67) pg/ml, ( 94.32 ± 28.42 ) pg/ml, respectively, and was significantly higher than that in control group [ ( 10.42 ±5.86) pg/ml, (8.58 ±4.86) pg/ml, (8.22 ±4.58) pg/ml, P <0.01 ].There was no MCP-1 protein expression in the control group;MCP-1 protein was detected in the successful induction group, and MCP-1 expression was positively correlated with expressions of the PDGF, TGF-β1, α-SMA and NF-κB.Conclusions MCP-1 may play an important role in the course of pancreatic fibrosis in chronic obstructive pancreatitis.