CAJ | 학술논문

目的探讨子痫前期孕妇血浆内源性一氧化氮合酶抑制物--非对称性二甲基精氨酸(ADMA)的水平变化及胎盘组织中一氧化氮合酶抑制物水解酶--二甲基精氨酸二甲胺水解酶2(DDAH-2)的表达变化与子痫前期发病的关系.方法选择2004年1月至2005年1月在广州医学院第三附属医院住院分娩的子痫前期孕妇30例(子痫前期组),以同期正常晚期妊娠妇女10例(正常妊娠组)为对照.采用高效液相色谱(HPLC)分析法测定两组孕妇血浆ADMA水平;采用荧光定量PCR技术检测两组孕妇胎盘组织中DDAH-2 mRNA的表达.结果 (1)血浆ADMA水平:子痫前期组孕妇血浆ADMA水平为(18.0±7.2)mg/L,高于正常妊娠组的(10.3±1.7)mg/L,两组比较,差异有统计学意义(P<0.01);子痫前期组中,发病孕周<34周者血浆ADMA水平为(22.0±7.0)mg/L,显著高于发病孕周≥34周者的(12.7±2.8)ms/L,两者比较,差异也有统计学意义(P<0.01).(2)胎盘组织DDAH-2 mRNA的表达量:子痫前期组胎盘组织中DDAH-2 mRNA的表达量为1×10(5.23±0.45) copy/μ,显著低于正常妊娠组的1×10(5.65±0.08)copy/μ,两组比较,差异有统计学意义(P<0.01);子痫前期组中,发病孕周<34周者胎盘组织DDAH-2 mRNA表达量为1×10(5.02±0.46)copy/μ,显著低于发病孕周≥34周者的1 ×10(5.61±0.19)copy/μ,两者比较,差异也有统计学意义(P<0.01).结论胎盘组织中DDAH-2 mRNA的低表达与血浆ADMA水平的升高,可能与子痫前期的发病有关.
목적 탐토자간전기잉부혈장내원성일양화담합매억제물--비대칭성이갑기정안산(ADMA)적수평변화급태반조직중일양화담합매억제물수해매--이갑기정안산이갑알수해매2(DDAH-2)적표체변화여자간전기발병적관계.방법 선택2004년1월지2005년1월재엄주의학원제삼부속의원주원분면적자간전기잉부30례(자간전기조),이동기정상만기임신부녀10례(정상임신조)위대조.채용고효액상색보(HPLC)분석법측정량조잉부혈장ADMA수평;채용형광정량PCR기술검측량조잉부태반조직중DDAH-2 mRNA적표체.결과 (1)혈장ADMA수평:자간전기조잉부혈장ADMA수평위(18.0±7.2)mg/L,고우정상임신조적(10.3±1.7)mg/L,량조비교,차이유통계학의의(P<0.01);자간전기조중,발병잉주<34주자혈장ADMA수평위(22.0±7.0)mg/L,현저고우발병잉주≥34주자적(12.7±2.8)ms/L,량자비교,차이야유통계학의의(P<0.01).(2)태반조직DDAH-2 mRNA적표체량:자간전기조태반조직중DDAH-2 mRNA적표체량위1×10(5.23±0.45) copy/μ,현저저우정상임신조적1×10(5.65±0.08)copy/μ,량조비교,차이유통계학의의(P<0.01);자간전기조중,발병잉주<34주자태반조직DDAH-2 mRNA표체량위1×10(5.02±0.46)copy/μ,현저저우발병잉주≥34주자적1 ×10(5.61±0.19)copy/μ,량자비교,차이야유통계학의의(P<0.01).결론 태반조직중DDAH-2 mRNA적저표체여혈장ADMA수평적승고,가능여자간전기적발병유관.
Objective To investigate the role of dimethylarginine dimethylaminohydrolase-2 (DDAH-2)/asymmetric dimethylarginine(ADMA)in pathophiology of preeclampsia by detecting expression of DDAH-2 in placenta and serum plasma ADMA.Methods From Jan.2004 to Jan.2005,30 preeclampsia patients(PE group)were chosen in the Third Affiliated Hospital.Guangzhou Medical College matched with 10 normal third trimester women as control(control group).The placental DDAH-2 mRNA expression was detected by fluorescence quantitative polymerase chain reaction(FQ-PCR)and the plasma concentration of ADMA WSB determined by high performance liquid chromatography(HPLC).Results(1)The level of ADMA in PE group was significantly higher that than of control group[(18.0±7.2)mg/L vs.(10.3±1.7)mg/L,P<0.01].The expression level of ADMA in preeclampsia occurring before 34 gestatinal weeks WaS significantly higher than that of preeclampsia occurring after 34 gestational weeks[(22.0±7.0)ms/L vs.(12.7±2.8)mg/L,P<0.01].(2)The Placental DDAH-2 mRNA expression in preeclampsia patients was remarkably lower than that of control group[1×10(5.23±0.45)copy/μlvs.1×10(5.65±0.08)copy/μl,P<0.01].The Placental DDAH-2 mRNA in preeclampsia occurring before 34 gestatinal weeks was significantly lower than that of preeclampsia occurring after 34 gestational weeks [1×10(5.02±0.46)copy/μl vs.1×10(5.61±0.19)copy/μl,P<0.01].Conclusion Our results suggested that low expression of DDAH-2 in placenta and increased serum ADMA level might confer the susceptibility to preeclampsia.lampia occurri