中华神经科杂志
中華神經科雜誌
중화신경과잡지
Chinese Journal of Neurology
2008年
6期
371-375
,共5页
黄清%杨期东%刘运海%夏琨%朱赞华%葛亮%刘宁%谭若利%唐建生%王玮
黃清%楊期東%劉運海%夏琨%硃讚華%葛亮%劉寧%譚若利%唐建生%王瑋
황청%양기동%류운해%하곤%주찬화%갈량%류저%담약리%당건생%왕위
脑梗塞%膜糖蛋白类%肿瘤坏死因子类%多态性,单核苷酸%聚合酶链反应
腦梗塞%膜糖蛋白類%腫瘤壞死因子類%多態性,單覈苷痠%聚閤酶鏈反應
뇌경새%막당단백류%종류배사인자류%다태성,단핵감산%취합매련반응
Brain infarction%Membrane glycoproteins%Tumor necrosis factors%Polymorphism,single nucleotide%Polymerase chain reaction
目的 探讨肿瘤坏死因子超家族成员4(TNFSF4)基因SNP rs3861950与本地区脑梗死发病的关系.方法 采用TaqMan-PCR方法检测TNFSF4基因SNP rs3861950基因型与等位基因频率.湖南籍汉族脑梗死共287例,对照组为湖南籍汉族健康体检者共285名.结果 脑梗死组CC基因型(7.7%)分布与对照组(2.1%)相比,差异具有统计学意义(X2=9.553,P=0.008);C等位基因频率脑梗死组(0.190)高于对照组(0.137,X2=5.887,P=0.015).动脉血栓性脑梗死亚组与对照组比较其基因型(X2=9.396,P=0.009)及等位基因频率(X2=6.134,P=0.013)差异均有统计学意义.非条件Logistic多因素回归分析显示CC基因型使脑梗死发病的风险是其他危险因素的3.7倍(P=0.002,OR 3.706).结论 TNFSF4基因rs3861950 C→T与湖南籍汉族脑梗死发病相关,尤其与动脉血栓性脑梗死发病相关,rs3861950 C等位基因可能是湖南籍汉族脑梗死发病的独立危险因素.
目的 探討腫瘤壞死因子超傢族成員4(TNFSF4)基因SNP rs3861950與本地區腦梗死髮病的關繫.方法 採用TaqMan-PCR方法檢測TNFSF4基因SNP rs3861950基因型與等位基因頻率.湖南籍漢族腦梗死共287例,對照組為湖南籍漢族健康體檢者共285名.結果 腦梗死組CC基因型(7.7%)分佈與對照組(2.1%)相比,差異具有統計學意義(X2=9.553,P=0.008);C等位基因頻率腦梗死組(0.190)高于對照組(0.137,X2=5.887,P=0.015).動脈血栓性腦梗死亞組與對照組比較其基因型(X2=9.396,P=0.009)及等位基因頻率(X2=6.134,P=0.013)差異均有統計學意義.非條件Logistic多因素迴歸分析顯示CC基因型使腦梗死髮病的風險是其他危險因素的3.7倍(P=0.002,OR 3.706).結論 TNFSF4基因rs3861950 C→T與湖南籍漢族腦梗死髮病相關,尤其與動脈血栓性腦梗死髮病相關,rs3861950 C等位基因可能是湖南籍漢族腦梗死髮病的獨立危險因素.
목적 탐토종류배사인자초가족성원4(TNFSF4)기인SNP rs3861950여본지구뇌경사발병적관계.방법 채용TaqMan-PCR방법검측TNFSF4기인SNP rs3861950기인형여등위기인빈솔.호남적한족뇌경사공287례,대조조위호남적한족건강체검자공285명.결과 뇌경사조CC기인형(7.7%)분포여대조조(2.1%)상비,차이구유통계학의의(X2=9.553,P=0.008);C등위기인빈솔뇌경사조(0.190)고우대조조(0.137,X2=5.887,P=0.015).동맥혈전성뇌경사아조여대조조비교기기인형(X2=9.396,P=0.009)급등위기인빈솔(X2=6.134,P=0.013)차이균유통계학의의.비조건Logistic다인소회귀분석현시CC기인형사뇌경사발병적풍험시기타위험인소적3.7배(P=0.002,OR 3.706).결론 TNFSF4기인rs3861950 C→T여호남적한족뇌경사발병상관,우기여동맥혈전성뇌경사발병상관,rs3861950 C등위기인가능시호남적한족뇌경사발병적독립위험인소.
Objective To investigate the association of the tumor necrosis factor ligand superfamily member(TNFSF)4 gene polymorphisms rs3861950 and cerebral infaretion in Hunan area.Methods The frequencies of the genotypes of rs3861950 were detected utilizing real-time fluorescent PCR method based on TaqMan probe.Subiects examined were composed of 287 patients and 285 healthy individuals.Results There were significant differences in TNFSF4 gene rs3861950 C→T site polymorphism and allele frequency between the subjects and the controls,the distribution of CC genotype was significantly higher in the former (7.7%)than in the later(2.1%,X2=9.553,P=0.008),so was the frequencies of C allele(0.190 vs 0.137,X2=5.887,P=0.015).Moreover,there were significant differences in the distribution of genotype and the frequencies of allele between the subgroup of cerebral thrombosis and control group ( for the distribution of genotype X2=9.396 P=0.009,for the frequencies of allele X2=6.134,P=0.013).Logistic regression analysis showed that the risk factor of CC genotype was 3.7 times higherthan others(P=0.002.OR 3.706).Conclusions TNFSF4 gene SNP rs3861950 is associated with cerebral infarction and C allele is considered to be one of independent risk factors in the Han population in Hunan Province.
