CAJ | 학술논문

TCP基因是一类植物中新发现的、可能具有转录因子活性的基因家族,成员包括金鱼草的Cyclodiea (Cyc)、玉米的Teosinte Branched1 (TB1)以及水稻中的PCF1、PCF2等.玉米的TB1基因有维持玉米顶端优势的作用,与分蘖的发生密切相关;水稻和玉米同属禾本科,在发育的过程中都有分蘖的发生.通过筛选水稻的基因组文库,得到了水稻中的一个TB1同源基因Oryza sativa Teosinte Branched1 (OsTB1).该基因不含内含子,基因编码一个长度为388个氨基酸的蛋白,在氨基酸水平上与TB1的同源性为70%,含有保守的TCP区和R区,是属于TCP基因家族的一个成员.RT-PCR和mRNA原位杂交分析结果表明,OsTB1在水稻的侧芽中有很强的表达,在花序中有较弱的表达.以上结果显示该基因可能在水稻侧芽和花序的起始和发育过程中起重要作用.
TCP기인시일류식물중신발현적、가능구유전록인자활성적기인가족,성원포괄금어초적Cyclodiea (Cyc)、옥미적Teosinte Branched1 (TB1)이급수도중적PCF1、PCF2등.옥미적TB1기인유유지옥미정단우세적작용,여분얼적발생밀절상관;수도화옥미동속화본과,재발육적과정중도유분얼적발생.통과사선수도적기인조문고,득도료수도중적일개TB1동원기인Oryza sativa Teosinte Branched1 (OsTB1).해기인불함내함자,기인편마일개장도위388개안기산적단백,재안기산수평상여TB1적동원성위70%,함유보수적TCP구화R구,시속우TCP기인가족적일개성원.RT-PCR화mRNA원위잡교분석결과표명,OsTB1재수도적측아중유흔강적표체,재화서중유교약적표체.이상결과현시해기인가능재수도측아화화서적기시화발육과정중기중요작용.
TCP genes, including Teosinte Branched 1(TB1) in maize, Cyclodiea (Cyc) in snapdragon and PCF1 and PCF2 in rice etc., constitute a newly discovered gene family which encode transcription regulators in plants. TB1 controls the apical dominance in maize and is involved in the maize tiller development. Both rice and maize belong to the Gramineae and can produce tillers. By screening a rice genomic library, we isolated a TB1-like gene-Oryza sativa Teosinte Branched (OsTB1) in rice. We also isolated the cDNA of OsTB1 by RT-PCR. The OsTB1 gene, with no intron, encodes a polypeptide consisting of 388 amino acids. OsTB1 shows 70% amino acid sequence similarity with TB1 and has the conserved TCP and R regions. This gene is a newly discovered gene of the TCP gene family in rice. RT-PCR and mRNA in situ hybridization showed that OsTB1 strongly expressed in rice axillary buds and weakly in inflorescence. OsTB1 is suggested to be related to axillary bud and inflorescence development in rice.