中华临床免疫和变态反应杂志
中華臨床免疫和變態反應雜誌
중화림상면역화변태반응잡지
CHINESE JOURNAL OF ALLERGY & CLINICAL IMMUNOLOGY
2009年
2期
95-99
,共5页
PCR技术%链格孢霉
PCR技術%鏈格孢黴
PCR기술%련격포매
PCR%Alternaria dternata
目的 应用PER技术对链格孢霉进行鉴定,以期获得制备链格孢霉变应原制剂的纯菌种.方法 采用空气曝皿收获链格孢霉菌种并进行培养,应用PER技术对其进行鉴定.结果 经空气曝皿、形态学鉴定为链格孢霉菌株的PER产物经1.5%琼脂糖凝胶电泳分析,紫外灯下鉴定扩增所得条带约580 bp;测序结果经Blast比对,544个碱基中543个能够与链格孢霉26s rDNA的545个碱基比对上,缺失1个,不能识别1个,相符率达99%.结论 证实待检真菌为链格孢霉.
目的 應用PER技術對鏈格孢黴進行鑒定,以期穫得製備鏈格孢黴變應原製劑的純菌種.方法 採用空氣曝皿收穫鏈格孢黴菌種併進行培養,應用PER技術對其進行鑒定.結果 經空氣曝皿、形態學鑒定為鏈格孢黴菌株的PER產物經1.5%瓊脂糖凝膠電泳分析,紫外燈下鑒定擴增所得條帶約580 bp;測序結果經Blast比對,544箇堿基中543箇能夠與鏈格孢黴26s rDNA的545箇堿基比對上,缺失1箇,不能識彆1箇,相符率達99%.結論 證實待檢真菌為鏈格孢黴.
목적 응용PER기술대련격포매진행감정,이기획득제비련격포매변응원제제적순균충.방법 채용공기폭명수획련격포매균충병진행배양,응용PER기술대기진행감정.결과 경공기폭명、형태학감정위련격포매균주적PER산물경1.5%경지당응효전영분석,자외등하감정확증소득조대약580 bp;측서결과경Blast비대,544개감기중543개능구여련격포매26s rDNA적545개감기비대상,결실1개,불능식별1개,상부솔체99%.결론 증실대검진균위련격포매.
Objective To produe the allergen extracts with Alternaria alternarta while were identified by PCR technique. Methods Alternaria alternata was collected from air and cultured under 26℃, identified by PCR technique. Result The fungus that was collected from air and identified as A lternaria alternata by morphology, its 26srDNA was amplified by PCR, resulting a 580 bp band, after sequencing, under microscope there were 543 bases those could be identical and consistent to the Alternaria alternata data from intemet based on the result of blast comparison, corresponding rate is 99%. Conclusion We succeeded in collecting and producing Alternaria alternata.
