CAJ | 학술논문

为探讨NaCl胁迫与水分胁迫对番茄蔗糖秘累影响的异同,以番茄(Lycopersicon esculentum Mill.)品种辽园多丽为试材,采用营养液水培法,以50 mmol·L~(-1) NaCl及等渗的聚乙二醇(PEG-6000)为处理,研究了番茄幼曲叶片中糖含量和蔗谢代谢相关酶活性的变化.结果表明:等渗的NaCl和PEG处理均可提高番茄幼苗叶片中葡萄糖和果糖的含量,处理12d时果糖含量分别比CK提高了20.2%和32.2%;葡萄糖含量分别比CK提高了12.0%和18.9%;等渗的NaCl和PEG处理均提高了酸性转化酶活性,处理12d时分别比对照高12.34%和17.76%;PEG胁迫下番茄幼苗叶片中葡萄糖和果糖的含量及转化酶的活性高于NaCl胁迫.可见,盐胁迫和渗透胁迫下番茄叶片葡萄糖和果糖的积累主要是由于转化酶活性的提高引起的;与盐胁迫相比渗透胁迫下番茄叶片积累更多的可溶性糖,从而说明NaCl胁迫对番肺叶片糖代谢的影响主要是渗透胁迫.
위탐토NaCl협박여수분협박대번가자당비루영향적이동,이번가(Lycopersicon esculentum Mill.)품충료완다려위시재,채용영양액수배법,이50 mmol·L~(-1) NaCl급등삼적취을이순(PEG-6000)위처리,연구료번가유곡협편중당함량화자사대사상관매활성적변화.결과표명:등삼적NaCl화PEG처리균가제고번가유묘협편중포도당화과당적함량,처리12d시과당함량분별비CK제고료20.2%화32.2%;포도당함량분별비CK제고료12.0%화18.9%;등삼적NaCl화PEG처리균제고료산성전화매활성,처리12d시분별비대조고12.34%화17.76%;PEG협박하번가유묘협편중포도당화과당적함량급전화매적활성고우NaCl협박.가견,염협박화삼투협박하번가협편포도당화과당적적루주요시유우전화매활성적제고인기적;여염협박상비삼투협박하번가협편적루경다적가용성당,종이설명NaCl협박대번폐협편당대사적영향주요시삼투협박.
Tomato (Lycopersicon esculentum Mill.) cultivar 'Liaoyuan Duoli' was used by the method of water culture. It was respectively treated with 50 mmol·L~(-1) NaCl and PEG-6000 Iso-osmotic. The contents of soluble sugars and the related enzyme activities of sucrose metabolism were determined. The results showed that NaCl and PEG-6000 Iso-osmotic stresses enhanced the contents of fructose and glucose in tomato leaves, increased the activities of AI and NI, reduced the activities of SS and SPS.The effects of two stresses on sucrose contents were weak. The contents of fructose and glucose and invertase activities were higher under PEG stress than that under NaCl stress. These results suggested that NaCl and PEG stresses enhanced the contents of fructose and glucose in tomato leaves, which is due to changing that the activity of invertase activity affecting sugar accumulation. The contents of soluble sugars were higher under PEG stress than that under NaCl stress.