CAJ | 학술논문

目的观察不同剂量吡喹酮对曼氏裂头蚴感染小鼠的疗效.方法 156只昆明小鼠分2批感染,每只经口(灌胃)接种感染5条裂头蚴.第l批36只小鼠分为6组(每组6只),1-5组小鼠分别接种含不同浓度吡喹酮培养液中培养3 d的裂头蚴,6组小鼠接种正常培养液中培养3 d的裂头蚴,作为对照组;第2批120只小鼠分成12组(每组10只),分别接种青蛙或蝌蚪体内的裂头蚴,1-9组于感染后1周或5周用不同剂量的吡喹酮灌胃治疗,10-12组为对照组.各组小鼠于治疗结束后1周或2周剖杀,收集裂头蚴数并计算各组的平均检出虫数和减虫率.结果第1批小鼠接种在100-40μg,/ml吡喹酮中培养3 d的裂头蚴后,检出虫数与对照组相比差异无统计学意义(P>0.05),接种在50 μg/ml吡喹酮中培养3 d的裂头蚴后,减虫率仅为16.60%,各剂量组减虫率之间差异无统计学意义(P>0.05).第2批小鼠感染青蛙体内裂头蚴1周后,用200、400、800 mg/kg吡喹酮治疗后1周和2周的检出虫数与对照组相比,差异均无统计学意义(P均>0.05),相同剂量组治疗后1周和2周后减虫率差异均无统计学意义(P均>0.05);感染蝌蚪体内裂头蚴1周后,200、400 mg/kg吡喹酮治疗后1周检出虫数与对照组相比差异均无统计学意义(P均>0.05),800 mg/kg治疗后l周减虫率为17.02%,各剂量组减虫率之间差异无统计学意义(P>0.05).小鼠感染青蛙体内裂头蚴5周后,用1 200、1 800 me/kg吡喹酮治疗1周和2周后,检出虫数差异无统计学意义(P>0.05),但均明显高于对照组(P均<0.05),相同剂量组减虫率之间的差异无统计学意义(P>0.05).结论吡喹酮(10～50 μg/ml)在体外对裂头蚴无明显杀伤作用,但大剂量(1 800mg/kg)灌胃时对裂头蚴感染小鼠疗效较好.
목적 관찰불동제량필규동대만씨렬두유감염소서적료효.방법 156지곤명소서분2비감염,매지경구(관위)접충감염5조렬두유.제l비36지소서분위6조(매조6지),1-5조소서분별접충함불동농도필규동배양액중배양3 d적렬두유,6조소서접충정상배양액중배양3 d적렬두유,작위대조조;제2비120지소서분성12조(매조10지),분별접충청와혹과두체내적렬두유,1-9조우감염후1주혹5주용불동제량적필규동관위치료,10-12조위대조조.각조소서우치료결속후1주혹2주부살,수집렬두유수병계산각조적평균검출충수화감충솔.결과 제1비소서접충재100-40μg,/ml필규동중배양3 d적렬두유후,검출충수여대조조상비차이무통계학의의(P>0.05),접충재50 μg/ml필규동중배양3 d적렬두유후,감충솔부위16.60%,각제량조감충솔지간차이무통계학의의(P>0.05).제2비소서감염청와체내렬두유1주후,용200、400、800 mg/kg필규동치료후1주화2주적검출충수여대조조상비,차이균무통계학의의(P균>0.05),상동제량조치료후1주화2주후감충솔차이균무통계학의의(P균>0.05);감염과두체내렬두유1주후,200、400 mg/kg필규동치료후1주검출충수여대조조상비차이균무통계학의의(P균>0.05),800 mg/kg치료후l주감충솔위17.02%,각제량조감충솔지간차이무통계학의의(P>0.05).소서감염청와체내렬두유5주후,용1 200、1 800 me/kg필규동치료1주화2주후,검출충수차이무통계학의의(P>0.05),단균명현고우대조조(P균<0.05),상동제량조감충솔지간적차이무통계학의의(P>0.05).결론 필규동(10～50 μg/ml)재체외대렬두유무명현살상작용,단대제량(1 800mg/kg)관위시대렬두유감염소서료효교호.
Objective To observethe efficacy of mice infected with Sparganum mansoni by using different dosages of praziquantel.Methods A total of 156 Kunming mice were divided into 2 batches.each of them wag orally infted with 5 spargana.Thirty-six mice in the first batch were equally divided into 6 groups.the mice in group 1-5 were inoculated with spargana cultured in different concentrations of praziquantel for 3 days,the group 6 served as a control.One hundred and twenty mice in the second batch were equally divided into 12 groups,each mouse was inoculated with spargana obtained from frogs or tadpoles,group 1-9 were treated by different desages of praziquantel 1 or5 weeks post infection.group 10-12 served as controls.All of the mice wore sacriftced and dissectedl or 2 weeks after the treatment.the mean number of worms recovered was cmculated and worm reduction rates were determined.Results The number of worm recovered from mice infected with spargana cultured in 10-40 μg/ml of praziquantel had no significant difference with that of the control(P>0.05).The worm reduction rate wag 16.60%while the spargana beins cultured in 50 μg/ml of praziquantel.The worm reduction rates of the mice that sacrificed 1 week or 2 weeks after being treated by the same dosage of praziquantel had no significant difference(P>0.05).When being treated with 200.400 or 800 mg/kg of praziquantel 1 week post infection,the number of worm recovered from mice infected with spargana from frogs had no significant difference with those of the control 1 and 2 weeks after the treatment(P>0.05).The worm reduction rates between the groups with the same dosage 1 week and 2 weeks post treatment had no significant difference(P>0.05).When being treated with 200 or 400 mg/kg of praziquantel 1 week post infection,the number of worm recovered from mice infected with spargana from tadpoles had no statistically difference with that of the control 1 week after treatment (P>0. 05). The worm reduction rate of mice was only 17.02% while being treated with 800 mg/kg of praziquantel. The worm reduction rates among groups with different dosages had no significant difference (P>0.05). Compared with the mice infected with spargana from frogs treated with 1 200 or 1 800 mg/kg of praziquantel 5 weeks post infection, the difference between the numbers of worm recovered from mice 1 week and 2 weeks after treatment had no statistically significance (P > 0.05), but they were significantly higher than those of the controls (P<0.05 ). The worm reduction rates among the groups with the same dosage had no significant difference (P>0.05). Conclusions Praziquantel (10-50 μg/ml) has no evident killing effect on spargnna in vitro, but when the dosage is higher(1 800 mg/kg), it has certain efficacy for treating the mice infected with spargana by oral inoculation.