CAJ | 학술논문

采用ELISA定量测定法研究了转EPSPs基因大豆不同生长时期不同器官中CP4 EPSPS蛋白含量的变化.结果表明:转EPSPS基因大豆不同器官在不同牛育期CP4 EPSPS蛋白含量表现出较大的差异,R8期籽粒中的CP4EPSPS蛋白含量在所有时期和所有组织中蛋门含世最高;上位叶和下位叶中CP4 EPSPS蛋白除V3～V5期和R8期外的表达趋势一致,茎上部和茎下部中CP4 EPSPS蛋白在不同时期表达动态趋势基本一致,根中CP4 EPSPS蛋白含量存V3～V5期下降,然后逐渐升高,R1～R8期有一个大幅度的下降过程.从V1期至R8期,随着植株的不断生长,各组织中CP4 EPSPS蛋白的含量有明显的变化.
채용ELISA정량측정법연구료전EPSPs기인대두불동생장시기불동기관중CP4 EPSPS단백함량적변화.결과표명:전EPSPS기인대두불동기관재불동우육기CP4 EPSPS단백함량표현출교대적차이,R8기자립중적CP4EPSPS단백함량재소유시기화소유조직중단문함세최고;상위협화하위협중CP4 EPSPS단백제V3～V5기화R8기외적표체추세일치,경상부화경하부중CP4 EPSPS단백재불동시기표체동태추세기본일치,근중CP4 EPSPS단백함량존V3～V5기하강,연후축점승고,R1～R8기유일개대폭도적하강과정.종V1기지R8기,수착식주적불단생장,각조직중CP4 EPSPS단백적함량유명현적변화.
The content of CP4 EPSPS protein in transgenic soybean plant was studied by Elisa method in this paper. Results showed that significant difference of CP4 EPSPS protein contents existed in different tissues and growth stages,with CP4 EPSPS protein content in seeds at maturity the highest. The contents of CP4 EPSPS protein in the upper and the bottom leaves were not significantly different in all growth stages except stages V3-V5 and R8. The contents of CP4 EPSPS protein in the upper and the bottom stalks showed consistent trend in different stages. The content of CP4 EPSPS protein in root decreased in stage V3-V5, then increased gradually,and then decreased from R1 to R8. With the growth of the plant, the content of the protein of CP4 EPSPS in all tissues changed greatly from VI to V8 stage.