农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2011年
5期
634-638
,共5页
佘永红%罗瑞明%刘晓连%柳杨
佘永紅%囉瑞明%劉曉連%柳楊
사영홍%라서명%류효련%류양
葡萄糖%抑制%分批补料发酵
葡萄糖%抑製%分批補料髮酵
포도당%억제%분비보료발효
Glucose%Inhibition%Fed-batch fermentation
[目的]探求获取高产量透明质酸的发酵方法.[方法]在微/物发酵法生产透明质酸的过程中,探索其底物浓度对发酵的影响.[结果]葡萄糖对透明质酸发酵的影响最大,低浓度的葡萄糖只能合成少量菌体和透明质酸,高浓度的葡萄糖会抑制菌体的生长和透明质酸的形成,通过探索实验得到分批补料发酵可以解除底物抑制,其具体方法为在发酵开始前加入2%的葡萄糖,14 h时加2%的葡萄糖,22h时再加2%的葡萄糖.[结论]在微生物发酵过程中,分批补料发酵能够解除底物抑制,得到相对分子质量较大的透明质酸,且产量高.
[目的]探求穫取高產量透明質痠的髮酵方法.[方法]在微/物髮酵法生產透明質痠的過程中,探索其底物濃度對髮酵的影響.[結果]葡萄糖對透明質痠髮酵的影響最大,低濃度的葡萄糖隻能閤成少量菌體和透明質痠,高濃度的葡萄糖會抑製菌體的生長和透明質痠的形成,通過探索實驗得到分批補料髮酵可以解除底物抑製,其具體方法為在髮酵開始前加入2%的葡萄糖,14 h時加2%的葡萄糖,22h時再加2%的葡萄糖.[結論]在微生物髮酵過程中,分批補料髮酵能夠解除底物抑製,得到相對分子質量較大的透明質痠,且產量高.
[목적]탐구획취고산량투명질산적발효방법.[방법]재미/물발효법생산투명질산적과정중,탐색기저물농도대발효적영향.[결과]포도당대투명질산발효적영향최대,저농도적포도당지능합성소량균체화투명질산,고농도적포도당회억제균체적생장화투명질산적형성,통과탐색실험득도분비보료발효가이해제저물억제,기구체방법위재발효개시전가입2%적포도당,14 h시가2%적포도당,22h시재가2%적포도당.[결론]재미생물발효과정중,분비보료발효능구해제저물억제,득도상대분자질량교대적투명질산,차산량고.
[Objective] The research aimed to search and obtain the fermentation method of hyaluronic acid with the high yield. [Method] In the production process of hyaluronic acid by the microbial fermentation method, the influence of substrate concentration on the fermentation was explored. [Result] The glucose had the biggest influence on the hyaluronic acid fermentation. The Iow concentration of glucose only could synthetize a little bacteria and hyaluronic acid, and the high concentration of glucose could inhibit the growth of bacteria and the formation of hyaluronic acid. Via the exploratory experiment, the fed-batch fermentation could disarm the substrate inhibition. The concrete method was adding 2% glucose before the start of fermentation, and adding 2% glucose at the 14th hour of fermentation, and adding 2% glucose again at the 22nd hour of fermentation. [Conclusion] In the microbial fermentation process, the fed-batch fermentation could disarm the substrate inhibition and obtain the hyaluronic acid with the larger relative molecular mass and the higher yield.