CAJ | 학술논문

目的研究抗原处理相关转运体蛋白1(transporter associated with antigen processing 1,TAP1)637A/G基因多态性与中国云南汉族鼻咽癌发病风险的关系,寻找鼻咽癌的易感基因.方法应用聚合酶链反应-限制性片段长度多态性对233例云南籍汉族鼻咽癌患者和296名云南籍汉族正常人对照组进行TAP1 637A/G基因多态性检测,采用PCR检测鼻咽癌患者EB病毒(EB virus,EBV)感染情况.计算各基因型与鼻咽癌发病风险以及与鼻咽癌患者EBV感染的相关性.结果 233例云南鼻咽癌患者中EBV感染率为58.4%,对照组EBV感染率为42.2%,差异有统计学意义(X~2=13.586,P<0.05).比较各基因型在两组中的分布,纯合子突变(GG型)、杂合子突变(AG型)和无突变者(AA型)分布差异均有统计学意义(P值均<0.05).与从基因型相比,携带GG、AG基因型罹患鼻咽癌的风险分别为4.26倍(95%CI为2.08～8.66,P<0.001)和1.56倍(95%CI为1.12～2.33,P<0.05);至少携带637G等位基因罹患鼻咽癌的风险为1.88倍(95%CI为1.35～2.82,P<0.001).TAP1三种基因型频率在EBV阳性组与EBV阴性组间比较,差异有统计学意义(P<0.05);在EBV阳性组中至少携带637G等位基因罹患鼻咽癌的风险为2.76倍(95%CI=1.69～4.63,P<0.001).结论 TAP1 637位点A/G基因多态性与云南汉族鼻咽癌遗传易感相关,TAP1基因多态性干扰人类白细胞抗原I型抗原的呈递可能与EBV感染致云南汉族鼻咽癌患病相关.
목적 연구항원처리상관전운체단백1(transporter associated with antigen processing 1,TAP1)637A/G기인다태성여중국운남한족비인암발병풍험적관계,심조비인암적역감기인.방법 응용취합매련반응-한제성편단장도다태성대233례운남적한족비인암환자화296명운남적한족정상인대조조진행TAP1 637A/G기인다태성검측,채용PCR검측비인암환자EB병독(EB virus,EBV)감염정황.계산각기인형여비인암발병풍험이급여비인암환자EBV감염적상관성.결과 233례운남비인암환자중EBV감염솔위58.4%,대조조EBV감염솔위42.2%,차이유통계학의의(X~2=13.586,P<0.05).비교각기인형재량조중적분포,순합자돌변(GG형)、잡합자돌변(AG형)화무돌변자(AA형)분포차이균유통계학의의(P치균<0.05).여종기인형상비,휴대GG、AG기인형리환비인암적풍험분별위4.26배(95%CI위2.08～8.66,P<0.001)화1.56배(95%CI위1.12～2.33,P<0.05);지소휴대637G등위기인리환비인암적풍험위1.88배(95%CI위1.35～2.82,P<0.001).TAP1삼충기인형빈솔재EBV양성조여EBV음성조간비교,차이유통계학의의(P<0.05);재EBV양성조중지소휴대637G등위기인리환비인암적풍험위2.76배(95%CI=1.69～4.63,P<0.001).결론 TAP1 637위점A/G기인다태성여운남한족비인암유전역감상관,TAP1기인다태성간우인류백세포항원I형항원적정체가능여EBV감염치운남한족비인암환병상관.
objective To study the relationship between 637A/G gene polymorphisms of the transporter associated with antigen processing 1 gene and nasopharyngeM carcinomas(NPC)and to find out the susceptible genes of NPC in Han population in Yunnan Province.China.Methods Two hundmd and thirtv-three cases with NPC and 296 cases matched cancer-free controls were genotyped for the TAP1 637 A/G polymorphism by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Epstein-Barr virus infection was detected by PCR.The adiusted edds ratios(OR)and 95%confidence intervals(CI)were calculated by using unconditional logistic regression model.Results Contrast with homozygous TAP1 637 AA.G allele significandy increasing risk of NPC was associated with homozygous 637GG OR=4.26(95%CI were 2.08-8.66.P<0.001)and heterozygous 637 AG OR=1.56(95% CI were 1.12-2.33,P<0.05).The subjects at least having one TAP1 637 G allele had OR of 1.88(95%CI were 1.35-2.82,P<0.001).Furthermore,EBV infection may increase the risk of developing NPC interacting with TAP1 637 A/Gpolymorphism OR=2.76(95%CI were 1.69-4.63,P.<0.001).Condusions The TAP1 637G allele is associated with the NPC in Han population in Yunnan China, EBV pathogenesis in NPC might be facilitated by polymorphisms in the TAP1 proteins.[ Key words ] Nasopharyngeal neoplasms; ATP-binding cassette transporter; Polymorphisms,genetic; Disease susceptibility; Epstein-barr virus infections