中华胃肠外科杂志
中華胃腸外科雜誌
중화위장외과잡지
CHINESE JOURNAL OF GASTROINTESTINAL SURGERY
2010年
8期
616-619
,共4页
结直肠肿瘤%血管性血友病因子%肿瘤转移%SW480细胞
結直腸腫瘤%血管性血友病因子%腫瘤轉移%SW480細胞
결직장종류%혈관성혈우병인자%종류전이%SW480세포
Colorectal neoplasms%von Willebrand factor%Neoplasm metastasis%SW480 cells
目的 探讨血管性血友病因子(vWF)对人结直肠癌细胞生长、黏附和迁移能力的影响.方法 体外培养人结直肠癌细胞株SW480,采用免疫细胞化学方法检测SW480细胞中vWF的表达情况.用vWF抗体对其进行处理,采用倒置显微镜观察细胞形态学变化;MTT法检测细胞增殖活性和对细胞外基质成分--Ⅳ型胶原黏附能力的变化;Transwell法检测细胞迁移能力的改变.结果 SW480细胞能够表达vWF,染色部位主要位于细胞核,细胞质亦有轻微表达.vWF抗体能够显著抑制SW480细胞的生长,该抑制作用呈剂量及时间依赖性(P<0.05);20 ms/L vWF抗体处理48 h后,SW480细胞的黏附力明显下降(P<0.05);迁移能力明显减弱[穿膜细胞数(54.60±11.01)比(97.27±10.01),P<0.01].结论 人结直肠癌细胞能够表达vWF;vWF对人结直肠细胞的生长、黏附、迁移过程中起重要促进作用.
目的 探討血管性血友病因子(vWF)對人結直腸癌細胞生長、黏附和遷移能力的影響.方法 體外培養人結直腸癌細胞株SW480,採用免疫細胞化學方法檢測SW480細胞中vWF的錶達情況.用vWF抗體對其進行處理,採用倒置顯微鏡觀察細胞形態學變化;MTT法檢測細胞增殖活性和對細胞外基質成分--Ⅳ型膠原黏附能力的變化;Transwell法檢測細胞遷移能力的改變.結果 SW480細胞能夠錶達vWF,染色部位主要位于細胞覈,細胞質亦有輕微錶達.vWF抗體能夠顯著抑製SW480細胞的生長,該抑製作用呈劑量及時間依賴性(P<0.05);20 ms/L vWF抗體處理48 h後,SW480細胞的黏附力明顯下降(P<0.05);遷移能力明顯減弱[穿膜細胞數(54.60±11.01)比(97.27±10.01),P<0.01].結論 人結直腸癌細胞能夠錶達vWF;vWF對人結直腸細胞的生長、黏附、遷移過程中起重要促進作用.
목적 탐토혈관성혈우병인자(vWF)대인결직장암세포생장、점부화천이능력적영향.방법 체외배양인결직장암세포주SW480,채용면역세포화학방법검측SW480세포중vWF적표체정황.용vWF항체대기진행처리,채용도치현미경관찰세포형태학변화;MTT법검측세포증식활성화대세포외기질성분--Ⅳ형효원점부능력적변화;Transwell법검측세포천이능력적개변.결과 SW480세포능구표체vWF,염색부위주요위우세포핵,세포질역유경미표체.vWF항체능구현저억제SW480세포적생장,해억제작용정제량급시간의뢰성(P<0.05);20 ms/L vWF항체처리48 h후,SW480세포적점부력명현하강(P<0.05);천이능력명현감약[천막세포수(54.60±11.01)비(97.27±10.01),P<0.01].결론 인결직장암세포능구표체vWF;vWF대인결직장세포적생장、점부、천이과정중기중요촉진작용.
Objective To study effect of von Willebrand factor (vWF) on the proliferation, adhesion and migration of human colorectal cancer cells. Methods Human colorectal cancer cell line SW480 was cultured in vitro, and the expression of vWF in SW480 cells was detected by immunocytochemistry. SW480 cells were treated with vWF antibody (vWFAb), and the morphological change was examined by inverted microscope. Cell proliferation and ability to adhere extracellular matrix IV collagen were detected with MTT. Migration ability of SW480 cells was assayed by Transwell. Results The human colorectal cancer cell line SW480 expressed vWF which was mainly in nucleus and slightly in cytoplasm. vWFAb significantly inhibited the proliferation ability of SW480 cells in dose- and time-dependent manner (P<0.05). After vWFAb treatment, SW480 cells adhesion decreased significantly (P<0.05), and transmembrane migration of cells significantly decreased (54.60± 11.01 vs 97.27±10.01,P<0.01). Conclusions Human colorectal cancer cells can express vWF. vWF in human colorectal cancer cells plays an important role in promoting proliferation, adhesion, and migration.
