CAJ | 학술논문

白血病患者与非亲缘造血干细胞供者的HLA-Cw基因高分辨分型
백혈병환자여비친연조혈간세포공자적HLA-Cw기인고분변분형
High resolution matching of HLA-Cw and effect on unrelated blood stem cell transplantation in acute leukemia

万方数据

中华器官移植杂志中華器官移植雜誌 중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2008年 3期 152-154 ,共3页

何军%陈子兴%侯建全%吴德沛%鲍晓晶%姚利%袁晓妮%岑建农%邱桥成%狄文英%张辉%张健%周晓华%徐惠新

하군%진자흥%후건전%오덕패%포효정%요리%원효니%잠건농%구교성%적문영%장휘%장건%주효화%서혜신

HLA-C抗原%组织相容性试验%造血干细胞移植%白血病 HLA-C抗原%組織相容性試驗%造血榦細胞移植%白血病
HLA-C항원%조직상용성시험%조혈간세포이식%백혈병
HLA-C antigens%Histocompatibility testing%Hematopoietic stem cell transplantation%Leukemia

目的对白血病患者与非亲缘造血干细胞供者进行HLA-Cw基因高分辨分型,探讨供、受者的相合几率.方法用序列特异性引物聚合酶链反应(PCR-SSP)联合序列特异性寡核苷酸探针(PCR-SSOP)方法进行HLA高分辨分型,依据分型结果将78例白血病患者与来自中国造血干细胞捐献者资料库中的126名供者进行配对,选择配合最佳者.结果 21例急性淋巴细胞白血病(ALL)患者中,HLA-Cw高分辨分型的常见基因型为Cw*0102、Cw*0304、Cw*0302、Cw*0702和Cw*0801,其表型频率分别为0.57、0.33、0.19、0.14和0.14;ALL患者的Cw*0102和Cw*0304的表型频率明显高于捐献者(P＜0.05);7例患者(33.3%,7/21)的10个等位基因与捐献者全相合,HLA-Cw全相合常见的基因亚位点依次为Cw*0102(4/7)、Cw*0702(2/7).34例慢性粒细胞白血病(CML)患者中,HLA-Cw高分辨分型的常见基因型为Cw*0702、Cw*0102、Cw*0304、Cw*0801、Cw*0401和Cw*0303,其表型频率分别为0.44、0.38、0.21、0.18、0.15和0.12;与捐献者相比,CML患者各基因型的表型频率的差异均无统计学意义;8例患者(23.5%,8/34)的10个等位基因与捐献者全相合,HLA-Cw全相合常见的基因亚位点依次为Cw*0702(5/8)、Cw*0304(3/8).23例急性髓系白血病(AML)患者中,4例的10个等位基因与捐献者全相合,其原发病均为AML-M2型.结论 ALL患者和捐献者的Cw*0102和Cw*0304基因表型频率较高,有利ALL患者寻找到HLA-Cw相合的供者.
목적 대백혈병환자여비친연조혈간세포공자진행HLA-Cw기인고분변분형,탐토공、수자적상합궤솔.방법 용서렬특이성인물취합매련반응(PCR-SSP)연합서렬특이성과핵감산탐침(PCR-SSOP)방법진행HLA고분변분형,의거분형결과장78례백혈병환자여래자중국조혈간세포연헌자자료고중적126명공자진행배대,선택배합최가자.결과 21례급성림파세포백혈병(ALL)환자중,HLA-Cw고분변분형적상견기인형위Cw*0102、Cw*0304、Cw*0302、Cw*0702화Cw*0801,기표형빈솔분별위0.57、0.33、0.19、0.14화0.14;ALL환자적Cw*0102화Cw*0304적표형빈솔명현고우연헌자(P＜0.05);7례환자(33.3%,7/21)적10개등위기인여연헌자전상합,HLA-Cw전상합상견적기인아위점의차위Cw*0102(4/7)、Cw*0702(2/7).34례만성립세포백혈병(CML)환자중,HLA-Cw고분변분형적상견기인형위Cw*0702、Cw*0102、Cw*0304、Cw*0801、Cw*0401화Cw*0303,기표형빈솔분별위0.44、0.38、0.21、0.18、0.15화0.12;여연헌자상비,CML환자각기인형적표형빈솔적차이균무통계학의의;8례환자(23.5%,8/34)적10개등위기인여연헌자전상합,HLA-Cw전상합상견적기인아위점의차위Cw*0702(5/8)、Cw*0304(3/8).23례급성수계백혈병(AML)환자중,4례적10개등위기인여연헌자전상합,기원발병균위AML-M2형.결론 ALL환자화연헌자적Cw*0102화Cw*0304기인표형빈솔교고,유리ALL환자심조도HLA-Cw상합적공자.
Objective To study high resolution typing of HLA-Cw and its effect on unrelated blood stem cell transplantation(BMT)in acute leukemia.Methods High resolution HLA-Cw analysis was performed on 78 patients(21 cases of ALL,34 cases of CML,23 cases of AML)from the data base of China Marrow Donor Program by using PCR-SSP and PCR-SSOP.Results In ALL patients,the HLA-Cw with common gene site was Cw*0102,Cw*0304,Cw*0302,Cw*0702,Cw*0801,and the phenotype frequencies were 0.57,0.33,0.19,0.14 and 0.14 respectively.The differences between these phenotype frequencies of Cw*0102 and Cw*0304 were statistically significant in ALL and donors(P＜0.05).Seven ALL patients(7/21)and donors were matched with loci in HLA-A,B,Cw,DRB1,DQB1,among which Cw*0102 was the subsites with highest frequency,followed by Cw*0702.In CML patients,the HLA-Cw with common gene site was Cw*0702,Cw*0102,Cw*0304,Cw*0801,Cw*0401,Cw*0303,and the phenotype frequencies were 0.44,0.38,0.21,0.18,0.15,0.12 respectively,with the differences between these phenotype frequencies being not statistically significant(P＞0.05).Eight CML patients(8/32)and donors were matched with loci in HLA-A,B,Cw,DR and DQ,among which Cw*0702 was the subsites with highest frequency,followed bv Cw*0304.All of the 4 M2 patients and donors were matched with loci in 23 cases of AML.Conclusion Cw*0102 and Cw*0304 have the obviously highest phenotype frequencies in ALL,which is beneficial for the selection of HLA-Cw matched donors.