中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2010年
5期
334-338
,共5页
袁寒艳%唐颖%雷玲%肖国兵%梁友信%王生%夏昭林
袁寒豔%唐穎%雷玲%肖國兵%樑友信%王生%夏昭林
원한염%당영%뢰령%초국병%량우신%왕생%하소림
职业暴露%多态性,单核苷酸%基因%椎间盘%基质金属蛋白酶
職業暴露%多態性,單覈苷痠%基因%椎間盤%基質金屬蛋白酶
직업폭로%다태성,단핵감산%기인%추간반%기질금속단백매
Occupational exposure%Polymorphism,single nucleotide%Genes%Intervertebral disk%Matrix metalloproteinases
目的 探索基质金属蛋白酶-3(MMP-3)、维生素D受体(VDR)的基因多态性与职业危险因素在腰椎间盘退变发生中的协同作用.方法 采用病例对照研究方法,对178名腰椎间盘退变患者及284名腰椎功能正常者进行职业危险因素问卷调查;同时应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测MMP-3与VDR的基因多态性,并应用相加模型分析基因多态性与职业危险因素在腰椎间盘退变中的协同作用.结果 非条件logistic回归分析显示,重度弯腰扭腰、全身振动及重体力负荷的OR值分别为4.06,8.96,5.46(P<0.05);含有MMP-3突变等位基因5A的6A5A/5A5A基因型及含有VDR-Apa突变等位基因A的AA/Aa基因型在病例组中的分布明显高于对照组,其OR值分别为1.96与1.70(P<0.05);相加模型分析显示,MMP-3的突变等位基因5A与全身振动、弯腰扭腰之间,以及VDR-Apa的突变等位基因A与弯腰扭腰之间存在协同作用(SI分别为13.27,2.91,2.35,P<0.05).结论 携带MMP-3的突变等位基因5A或VDR-Apa的突变等位基因A,同时暴露于职业危险因素全身振动或弯腰扭腰,可能增加发生腰椎间盘退变的危险性.
目的 探索基質金屬蛋白酶-3(MMP-3)、維生素D受體(VDR)的基因多態性與職業危險因素在腰椎間盤退變髮生中的協同作用.方法 採用病例對照研究方法,對178名腰椎間盤退變患者及284名腰椎功能正常者進行職業危險因素問捲調查;同時應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法檢測MMP-3與VDR的基因多態性,併應用相加模型分析基因多態性與職業危險因素在腰椎間盤退變中的協同作用.結果 非條件logistic迴歸分析顯示,重度彎腰扭腰、全身振動及重體力負荷的OR值分彆為4.06,8.96,5.46(P<0.05);含有MMP-3突變等位基因5A的6A5A/5A5A基因型及含有VDR-Apa突變等位基因A的AA/Aa基因型在病例組中的分佈明顯高于對照組,其OR值分彆為1.96與1.70(P<0.05);相加模型分析顯示,MMP-3的突變等位基因5A與全身振動、彎腰扭腰之間,以及VDR-Apa的突變等位基因A與彎腰扭腰之間存在協同作用(SI分彆為13.27,2.91,2.35,P<0.05).結論 攜帶MMP-3的突變等位基因5A或VDR-Apa的突變等位基因A,同時暴露于職業危險因素全身振動或彎腰扭腰,可能增加髮生腰椎間盤退變的危險性.
목적 탐색기질금속단백매-3(MMP-3)、유생소D수체(VDR)적기인다태성여직업위험인소재요추간반퇴변발생중적협동작용.방법 채용병례대조연구방법,대178명요추간반퇴변환자급284명요추공능정상자진행직업위험인소문권조사;동시응용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법검측MMP-3여VDR적기인다태성,병응용상가모형분석기인다태성여직업위험인소재요추간반퇴변중적협동작용.결과 비조건logistic회귀분석현시,중도만요뉴요、전신진동급중체력부하적OR치분별위4.06,8.96,5.46(P<0.05);함유MMP-3돌변등위기인5A적6A5A/5A5A기인형급함유VDR-Apa돌변등위기인A적AA/Aa기인형재병례조중적분포명현고우대조조,기OR치분별위1.96여1.70(P<0.05);상가모형분석현시,MMP-3적돌변등위기인5A여전신진동、만요뉴요지간,이급VDR-Apa적돌변등위기인A여만요뉴요지간존재협동작용(SI분별위13.27,2.91,2.35,P<0.05).결론 휴대MMP-3적돌변등위기인5A혹VDR-Apa적돌변등위기인A,동시폭로우직업위험인소전신진동혹만요뉴요,가능증가발생요추간반퇴변적위험성.
Objective To explore the synergistic interaction between MMP-3,VDR gene polymorphisms and occupational risk factors on lumbar disc degeneration. Methods A case-control study including 178 cases of lumbar disc degeneration and 284 controls was carried out through questionnaire and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology. Additive model was used to analyze the synergistic interaction between gene polymorphisms and occupational risk factors. Results The non-conditional logistic regression analysis showed that bending/twisting, whole-body vibration, heavy physical workload, alleles 5A of MMP-3(6A5A/5A5A) and A of VDR-Apa (AA /Aa) were significantly associated with lumbar disc degeneration (OR =4.06,8.96,5.46,1.96 and 1.70, respectively, P<0.05). There were synergistic interactions between the mutation genotype 5 A of MMP-3 and whole-body vibration exposure, between the mutation genotype 5A of MMP-3 and bending/twisting, and between the mutation genotype A of VDR-Apa and whole-body vibration exposure (SI: 13.27,2.91 and 2.35 respectively, P<0.05). Conclusion People with the mutation genotypes 5A of MMP-3 and/or A of VDR-Apa may have the increased risk of developing lumbar disc degeneration if they are exposed to whole-body vibration and/or bending/twisting.