CAJ | 학술논문

目的探讨他汀类药物对糖尿病大鼠肾脏NF-κB表达的影响及其可能机制.方法雄性SD大鼠60只,随机抽取40只腹腔注射链脲佐菌素65 mg/kg建立糖尿病模型,余20只为正常对照组(NC).成模大鼠随机分成两组,糖尿病非药物干预组(DM)及阿托伐他汀干预组(DA).DA组予阿托伐他汀2 mg/(kg·d)灌胃,NC组及DM组给予等量饮用水.大鼠12周后处死,取一肾组织抽提RNA,另一肾组织固定后做免疫组化.应用RT-PCR扩增NF-κB基因,比较3组大鼠的NF-κB mRNA表达.免疫组织化学方法检测NF-κB蛋白表达情况.结果RT-PCR结果示DM组大鼠肾组织中NF-κB mRNA表达较NC组大鼠明显增高(P＜0.05),DA组大鼠NF-κB的表达比DM组明显减少(P＜0.05).免疫组织化学结果显示NF-κB在肾小管及肾小球均有表达,DM组大鼠肾脏NF-κB阳性的细胞比NC组明显增多(P＜0.05),且着色较深.DA组的大鼠中,NF-κB阳性的细胞比DM组明显减少(P＜0.05),且着色较浅.结论阿托伐他汀能降低糖尿病大鼠肾组织中NF-κBmRNA及NF-κB蛋白质的表达,减缓肾组织病变进展.
목적 탐토타정류약물대당뇨병대서신장NF-κB표체적영향급기가능궤제.방법 웅성SD대서60지,수궤추취40지복강주사련뇨좌균소65 mg/kg건립당뇨병모형,여20지위정상대조조(NC).성모대서수궤분성량조,당뇨병비약물간예조(DM)급아탁벌타정간예조(DA).DA조여아탁벌타정2 mg/(kg·d)관위,NC조급DM조급여등량음용수.대서12주후처사,취일신조직추제RNA,령일신조직고정후주면역조화.응용RT-PCR확증NF-κB기인,비교3조대서적NF-κB mRNA표체.면역조직화학방법검측NF-κB단백표체정황.결과RT-PCR결과시DM조대서신조직중NF-κB mRNA표체교NC조대서명현증고(P＜0.05),DA조대서NF-κB적표체비DM조명현감소(P＜0.05).면역조직화학결과현시NF-κB재신소관급신소구균유표체,DM조대서신장NF-κB양성적세포비NC조명현증다(P＜0.05),차착색교심.DA조적대서중,NF-κB양성적세포비DM조명현감소(P＜0.05),차착색교천.결론아탁벌타정능강저당뇨병대서신조직중NF-κBmRNA급NF-κB단백질적표체,감완신조직병변진전.
Objective To explore the effects and mechanism of atrovastatin on the expression of the NF-κB in renal tissues of diabetic rats.Methods A total of 60 male SD rats was randomly taken out 40 rats to make diabetic model by injection of 65mg streptozotoein (STZ) into enterocoelia,the rest of 20 rats were normal control group.After the model made,atrovastatin (2 mg/kg/d) was given to the treated group,and the normal control group and diabetic rats without treatment group were given equivalent water.After 12 weeks,the rats were killed.Total RNA of the renal tissues was isolated from one kidney for each rat,and the renal tissues from the another kidney was prepared for immunohistochemistry (IHC) analysis.The NF-κB mRNA expressions among three groups were determined by RT-PCR.The distribution of NF-κB in the renal tissues was observed,and compared its difference among three groups.Results PCR showed that NF-κB mRNA was increased in the renal tissues of diabetic rats compared to control rats ( P ＜ 0.05 ).Drug-treated rats showed significantly decreased levels of NF-κB mRNA in the renal tissues compared to the untreated diabetic group( P ＜0.05).The results were also observed in protein lelel of NF-κB expression.IHC showed that there existed positive cells in the glomerular and renal tubulointerstitum.Conclusions Atrovastatin can down-regulate the expression of NF-κB and suppress the increased level of NF-κB protein in the renal tissue of diabetic rats,and slow the progress of retinopathy.