CAJ | 학술논문

目的研究外周血趋化因子及其受体表达变化在老年乳腺癌发病机制中的作用.方法应用双抗体夹心酶联免疫吸附试验(ELISA)检测30例老年乳腺癌患者(乳腺癌组)及30例健康老年人(对照组)血清活化后可调节正常T细胞表达和分泌因子(RANTES)、巨噬细胞炎性蛋白3α(MIP-3α)及间质细胞衍生因子1(SDF-1)的含量,应用流式细胞仪分析外周血CD3+T细胞表面CC趋化因子受体(CCR)5、CCR6及CXC趋化因子受体(CXCR)4的表达.结果乳腺癌组血清RANTES、MIP-3α及SDF-1含量明显高于对照组[(27.9±16.6)μg/L比(12.6±4.2)μg/L、(10.9±7.1) μg/L比(5.4±3.5)μg/L、(339.4±94.6) ng/L比(195.0±70.8) ng/L,P＜0.01]；两组外周血CD3+T 细胞表面CCR5、CCR6、CXCR4表达比较差异均无统计学意义(P＞0.05).结论外周血RANTES、MIP-3α及SDF-1异常升高可能在老年乳腺癌患者发病及进展过程中发挥作用.
목적 연구외주혈추화인자급기수체표체변화재노년유선암발병궤제중적작용.방법 응용쌍항체협심매련면역흡부시험(ELISA)검측30례노년유선암환자(유선암조)급30례건강노년인(대조조)혈청활화후가조절정상T세포표체화분비인자(RANTES)、거서세포염성단백3α(MIP-3α)급간질세포연생인자1(SDF-1)적함량,응용류식세포의분석외주혈CD3+T세포표면CC추화인자수체(CCR)5、CCR6급CXC추화인자수체(CXCR)4적표체.결과 유선암조혈청RANTES、MIP-3α급SDF-1함량명현고우대조조[(27.9±16.6)μg/L비(12.6±4.2)μg/L、(10.9±7.1) μg/L비(5.4±3.5)μg/L、(339.4±94.6) ng/L비(195.0±70.8) ng/L,P＜0.01]；량조외주혈CD3+T 세포표면CCR5、CCR6、CXCR4표체비교차이균무통계학의의(P＞0.05).결론 외주혈RANTES、MIP-3α급SDF-1이상승고가능재노년유선암환자발병급진전과정중발휘작용.
Objective To study the role of chemokines and their receptors in peripheral blood in the pathogenesis of breast cancer in elderly patients.Methods Serum levels of regulated on activation normal T-cell expressed and secreted (RANTES),macrophage inflammatory protein-3 α (MIP-3α ) and stromal cell derived factor-1 (SDF-1) were determined by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) in 30 elderly patients with breast cancer (breast cancer group) and 30 normal controls (control group).Meanwhile,chemokine receptors,CC chemokine receptor (CCR)5,CCR6 and CXC chemokine receptor(CXCR) 4 on peripheral blood CD3+ T-cell in two groups were analyzed by flow cytometry.Results Serum levels of RANTES,MIP-3 α and SDF-1 in breast cancer group were significantly higher than those in control group [ (27.9 ± 16.6) μ g/L vs.( 12.6 ± 4.2) μ g/L,( 10.9 ± 7.1 ) μ g/L vs.(5.4 ± 3.5 )μg/L,(339.4 ±94.6) ng/L vs. (195.0 ±70.8) ng/L,P ＜0.01].There was no significant difference in expressions of CCR5,CCR6 and CXCR4 on peripheral blood CD3+ T-cell between two groups (P ＞ 0.05).Conclusion The abnormal increase of serum levels of RANTES,MIP-3 α and SDF-1 in peripheral blood may play role in the carcinogenesis and progress of breast cancer in elderly patients.