中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2011年
22期
1-3
,共3页
孙艳荪%龙霞%史嵘%张帆%邓远飞%于洁
孫豔蓀%龍霞%史嶸%張帆%鄧遠飛%于潔
손염손%룡하%사영%장범%산원비%우길
糖尿病肾病%基因%解耦联蛋白2%多态性
糖尿病腎病%基因%解耦聯蛋白2%多態性
당뇨병신병%기인%해우련단백2%다태성
Diabetic nephropathies%Genes%Uncoupling protein 2%Polymorphism
目的 探讨解耦联蛋白2(UCP-2)基因启动子-866G/A多态性与中国人糖尿病肾病(DN)的关系.方法 182例2型糖尿病患者,其中并发DN90例(DN组),无肾病92例(NCD组),检测所有患者的基因组DNA,采用聚合酶链反应-限制性长度多态性方法,记录UCP-2等位基因及基因型频率并进行比较.结果 UCP-2基因启动子-866 G/A多态性在DN组中基因型频率分布,GG 14.44%(13/90),GA 31.11%(28/90),AA 54.44%(49/90),在NCD组中基因型频率分布,GG29.35%(27/92),GA 32.61%(30/92),AA 38.04%(35/92),两组比较差异有统计学意义(χ2=7.28,P＜ 0.05);NCD组UCP-2等位基因频率分布,G 45.65%(84/184),A 54.35%(100/184),DN组UCP-2等位基因频率分布,G 30.00%(54/180),A 70.00%(126/180),两组比较差异有统计学意义(χ2=9.47,P＜0.05).结论 UCP-2基因启动子-866G/A多态性与中国人DN相关,A/A基因型携带者DN发病率高.
目的 探討解耦聯蛋白2(UCP-2)基因啟動子-866G/A多態性與中國人糖尿病腎病(DN)的關繫.方法 182例2型糖尿病患者,其中併髮DN90例(DN組),無腎病92例(NCD組),檢測所有患者的基因組DNA,採用聚閤酶鏈反應-限製性長度多態性方法,記錄UCP-2等位基因及基因型頻率併進行比較.結果 UCP-2基因啟動子-866 G/A多態性在DN組中基因型頻率分佈,GG 14.44%(13/90),GA 31.11%(28/90),AA 54.44%(49/90),在NCD組中基因型頻率分佈,GG29.35%(27/92),GA 32.61%(30/92),AA 38.04%(35/92),兩組比較差異有統計學意義(χ2=7.28,P＜ 0.05);NCD組UCP-2等位基因頻率分佈,G 45.65%(84/184),A 54.35%(100/184),DN組UCP-2等位基因頻率分佈,G 30.00%(54/180),A 70.00%(126/180),兩組比較差異有統計學意義(χ2=9.47,P＜0.05).結論 UCP-2基因啟動子-866G/A多態性與中國人DN相關,A/A基因型攜帶者DN髮病率高.
목적 탐토해우련단백2(UCP-2)기인계동자-866G/A다태성여중국인당뇨병신병(DN)적관계.방법 182례2형당뇨병환자,기중병발DN90례(DN조),무신병92례(NCD조),검측소유환자적기인조DNA,채용취합매련반응-한제성장도다태성방법,기록UCP-2등위기인급기인형빈솔병진행비교.결과 UCP-2기인계동자-866 G/A다태성재DN조중기인형빈솔분포,GG 14.44%(13/90),GA 31.11%(28/90),AA 54.44%(49/90),재NCD조중기인형빈솔분포,GG29.35%(27/92),GA 32.61%(30/92),AA 38.04%(35/92),량조비교차이유통계학의의(χ2=7.28,P＜ 0.05);NCD조UCP-2등위기인빈솔분포,G 45.65%(84/184),A 54.35%(100/184),DN조UCP-2등위기인빈솔분포,G 30.00%(54/180),A 70.00%(126/180),량조비교차이유통계학의의(χ2=9.47,P＜0.05).결론 UCP-2기인계동자-866G/A다태성여중국인DN상관,A/A기인형휴대자DN발병솔고.
Objective To investigate the relationship between uncoupling protein 2 (UCP-2) gene promoter -866 G/A polymorphism and Chinese diabetic nephropathy (DN). Methods A total of 182 patients with type 2 diabetic mellitus were selected and divided into DN group (90 cases with DN ) and NCD group (92 cases without DN ). Genomic DNA was detected, and UCP-2 genotype and allele gene frequency was confirmed by polymerase chain reaction-restrictive fragment length polymorphism, then compared.Results The genotype frequency of UCP-2 gene promoter-866 G/A polymorphism was distributed in DN group[GG 14.44%( 13/90),GA 31.11%(28/90),AA 54.44%(49/90)],and distributed in NCD group[GG 29.35% ( 27/92 ), GA 32.61% ( 30/92 ), AA 38.04% ( 35/92 )], and there was significant difference between two groups ( χ2 = 7.28 , P ＜ 0.05 ). There was also significant difference in allele gene frequency between DN group and NCD group[G 45.65% (84/184) vs. 30.00% (54/180),A 54.35% (100/184) vs. 70.00% (126/180)]( χ2 = 9.47, P ＜ 0.05 ). Conclusion There is correlation between the UCP-2 gene promoter-866 G/A polymorphism and Chinese DN, and the incidence of DN with A/A genotype is increased.
