CAJ | 학술논문

目的观察心理应激及实施对抗措施对大鼠颞下颌关节(temporomandibular joint,TMJ)盘及翼外肌的影响,为临床防治颞下颌关节病(temporomandibular disorders,TMD)提供实验理论依据.方法采用交流箱心理应激大鼠动物模型,并在应激前后分别应用抗焦虑药物,去除应激源等措施.运用透射电镜、扫描电镜分别观察施加对抗措施前后空白对照组、心理应激组(PS组)、心理应激加注射抗焦虑药物地西泮组(PS +DI组)大鼠翼外肌和TMJ盘的微结构.结果透射电镜显示PS组1,3,5周时大鼠翼外肌出现程度不同的肌纤维线粒体水肿、基质密度降低、线粒体嵴减少；去除应激源恢复各组、PS+DI组1,3,5周肌纤维线粒体嵴和基质密度正常.扫描电镜显示PS组1周时大鼠关节盘表面部分滑膜脱落,3周时大鼠关节盘表面胶原纤维出现大小不等的条状磨损样变,5周关节盘表面胶原纤维错乱；去除应激源恢复各组、PS+ DI组1,3,5周关节盘无明显微结构改变.结论施加对抗措施能有效对抗心理应激对TMJ的影响,可为临床治疗应激引起的TMD提供借鉴.
목적 관찰심리응격급실시대항조시대대서섭하합관절(temporomandibular joint,TMJ)반급익외기적영향,위림상방치섭하합관절병(temporomandibular disorders,TMD)제공실험이론의거.방법 채용교류상심리응격대서동물모형,병재응격전후분별응용항초필약물,거제응격원등조시.운용투사전경、소묘전경분별관찰시가대항조시전후공백대조조、심리응격조(PS조)、심리응격가주사항초필약물지서반조(PS +DI조)대서익외기화TMJ반적미결구.결과 투사전경현시PS조1,3,5주시대서익외기출현정도불동적기섬유선립체수종、기질밀도강저、선립체척감소；거제응격원회복각조、PS+DI조1,3,5주기섬유선립체척화기질밀도정상.소묘전경현시PS조1주시대서관절반표면부분활막탈락,3주시대서관절반표면효원섬유출현대소불등적조상마손양변,5주관절반표면효원섬유착란；거제응격원회복각조、PS+ DI조1,3,5주관절반무명현미결구개변.결론 시가대항조시능유효대항심리응격대TMJ적영향,가위림상치료응격인기적TMD제공차감.
Objective To observe the influence of psychological stress and countermeasure implementation upon the temporomandibular joint ( TMJ ) articular disc and external pterygoid muscle in rats,providing experimental and theoretical evidence for clinical treatment of psychological stress-induced temporomandibular disorders (TMD).Methods The animal models treated by psychological stress induced by alternating current box were established.Before and after subjected to psychological stress,rats were given anxiolytic drugs to eliminate stressors.For all the rats in control group,psychological stress group ( PS group),and psychological stress plus anxiolytic drug injection group ( ( PS + DI) group),the microstructure of TMJ articular disc and external pterygoid muscle the changes in RNA expression of interleukin-1 ( 1L-1 ) were investigated by using transmission electron microscope (TEM),scanning electron microscope ( SEM ) and RT-PCR methods,respectively.Statistical analysis was performed upon the obtained test results.Results The TEM showed pathlogical changes in rats'pterygoid muscles of PS group at 1,3 and 5 weeks,including edema,reduction of substrate density and microchondrial cristae.Instead,these structures were all showed normal in the group of PS + DI and recovering group after removal of stressor.For the rats in PS group,SEM observation revealed that partial synovium of articular disc began to disintegrate 1 week after psychological stress.Strip-like wear degenerations were shown in the surface collagenous fiber in articular disc 3 weeks later,and the surface collagenous fibers in articular disc were arrayed in disorder 5 weeks following stress treatment.No significant microstructural changes in articular disc were observed in all stressor-eliminating groups and ( PS + DI) group 1,3,and 5 weeks following stress treatment.Statistical significance was noted in RNA expression level of IL-1 between PS group and PS + DI group (P＜0.05).In addition,there was significant difference in IL-1 expression between PS group and all stressor-eliminating groups.Conclusion The implementation of countermeasures effectively counteracted the influence upon TMJ induced by psychological stress,and provided possible resolutions for the clinical treatment of TMD induced by psychological stress.