中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2008年
12期
1631-1633
,共3页
染料木黄酮/药理学%乳腺肿瘤%细胞增殖%雌激素类
染料木黃酮/藥理學%乳腺腫瘤%細胞增殖%雌激素類
염료목황동/약이학%유선종류%세포증식%자격소류
Genistein/PD%Breast neoplasms%Cell proliferation%Estrogens
目的 探讨染料木黄酮(genistein,GEN)对人乳腺癌细胞增殖影响及作用机制.方法 MTT法测定GEN对雌激素依赖性乳腺癌细胞MCF-7和非雌激素依赖性乳腺癌细胞MDA-MB231增殖作用,应用雌激素受体拮抗剂IC1182780观察GEN的雌激素样作用途径,采用流式细胞术分析乳腺癌细胞周期情况.结果 GEN可促进MCF-7细胞的增殖,使G1期向S期转变,增殖作用可被雌激素受体拮抗剂所拮抗;对非雌激素依赖性MDA-MB231细胞增殖无影响.结论 GEN通过雌激素受体(ER)介导而发挥雌激素样活性作用.
目的 探討染料木黃酮(genistein,GEN)對人乳腺癌細胞增殖影響及作用機製.方法 MTT法測定GEN對雌激素依賴性乳腺癌細胞MCF-7和非雌激素依賴性乳腺癌細胞MDA-MB231增殖作用,應用雌激素受體拮抗劑IC1182780觀察GEN的雌激素樣作用途徑,採用流式細胞術分析乳腺癌細胞週期情況.結果 GEN可促進MCF-7細胞的增殖,使G1期嚮S期轉變,增殖作用可被雌激素受體拮抗劑所拮抗;對非雌激素依賴性MDA-MB231細胞增殖無影響.結論 GEN通過雌激素受體(ER)介導而髮揮雌激素樣活性作用.
목적 탐토염료목황동(genistein,GEN)대인유선암세포증식영향급작용궤제.방법 MTT법측정GEN대자격소의뢰성유선암세포MCF-7화비자격소의뢰성유선암세포MDA-MB231증식작용,응용자격소수체길항제IC1182780관찰GEN적자격소양작용도경,채용류식세포술분석유선암세포주기정황.결과 GEN가촉진MCF-7세포적증식,사G1기향S기전변,증식작용가피자격소수체길항제소길항;대비자격소의뢰성MDA-MB231세포증식무영향.결론 GEN통과자격소수체(ER)개도이발휘자격소양활성작용.
Objective To explore the role of genistein (genistcin, GEN) on human breast cancer cell proliferation and its mecha-nism. Method GEN MTT method was used to detect the proliferation of GEN on estrogen-dependent breast cancer cells MCF-7 and non-es-trogen-dependent breast cancer cell proliferation of MDA-MB231. Estrogen receptor.antagonist IC1182780 was used to observe the effect of GEN. FACS was used to analyze the cell cycle of breast cancer cells. Results GEN promote MCF-7 cell proliferation, and make G1/S transition. Estrogen receptor antagonist can block the proliferation effect on MCF-7 cells but no effect on MDA MB231. Conclusion GEN has estrogen-like activity, which was mediated by estrogen receptor (ER).
