中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2010年
3期
215-217
,共3页
李超%高成阁%蔺华利%陈良梅%张海燕%杨亚妮
李超%高成閣%藺華利%陳良梅%張海燕%楊亞妮
리초%고성각%린화리%진량매%장해연%양아니
精神分裂症%突触小体相关蛋白-25%基因%病例对照研究
精神分裂癥%突觸小體相關蛋白-25%基因%病例對照研究
정신분렬증%돌촉소체상관단백-25%기인%병례대조연구
Schizophrenia%Synaptosomal-associated protein 25%Gene%Case-control study
目的 研究突触小体相关蛋白-25(SNAP-25)基因3端翻译区单核苷酸多态性(SNP)位点rs8636与精神分裂症的关系.方法 采用聚合酶连反应-限制性片段长度多态性技术,检测300例精神分裂症患者(患者组)和300例正常对照(对照组)的基因型及基因频率.分析SNAP-25基因多态性与精神分裂症的相关性.结果 ①患者组与对照组rs8636多态性位点的等位基因和基因型的频数分布均符合Hardy-Weinberg平衡检验(X~2=2.69,v=1,P＞0.05;X~2=2.52,v=1,P＞0.05).②患者组与对照组SNAP-25基因rs8636多态性位点的基因型和等位基因频率的总体分布差异有统计学意义(X~2=6.454,P＜0.05;X~2=7.033,P＜0.05);③患者组C/C基因型频率(65.0%)和C等位基因频率(79.7%)高于对照组,差异有统计学意义(X~2=5.848,P＜0.05;OR=1.50);患者组T/T基因型频率(5.7%)略低于对照组,差异无统计学意义(X~2=2.453,P＞0.05).结论 SNAP-25基因rs8636多态性位点与精神分裂症可能存在关联.C/C基因型和C等位基因可能是精神分裂症发病的危险因素.
目的 研究突觸小體相關蛋白-25(SNAP-25)基因3耑翻譯區單覈苷痠多態性(SNP)位點rs8636與精神分裂癥的關繫.方法 採用聚閤酶連反應-限製性片段長度多態性技術,檢測300例精神分裂癥患者(患者組)和300例正常對照(對照組)的基因型及基因頻率.分析SNAP-25基因多態性與精神分裂癥的相關性.結果 ①患者組與對照組rs8636多態性位點的等位基因和基因型的頻數分佈均符閤Hardy-Weinberg平衡檢驗(X~2=2.69,v=1,P＞0.05;X~2=2.52,v=1,P＞0.05).②患者組與對照組SNAP-25基因rs8636多態性位點的基因型和等位基因頻率的總體分佈差異有統計學意義(X~2=6.454,P＜0.05;X~2=7.033,P＜0.05);③患者組C/C基因型頻率(65.0%)和C等位基因頻率(79.7%)高于對照組,差異有統計學意義(X~2=5.848,P＜0.05;OR=1.50);患者組T/T基因型頻率(5.7%)略低于對照組,差異無統計學意義(X~2=2.453,P＞0.05).結論 SNAP-25基因rs8636多態性位點與精神分裂癥可能存在關聯.C/C基因型和C等位基因可能是精神分裂癥髮病的危險因素.
목적 연구돌촉소체상관단백-25(SNAP-25)기인3단번역구단핵감산다태성(SNP)위점rs8636여정신분렬증적관계.방법 채용취합매련반응-한제성편단장도다태성기술,검측300례정신분렬증환자(환자조)화300례정상대조(대조조)적기인형급기인빈솔.분석SNAP-25기인다태성여정신분렬증적상관성.결과 ①환자조여대조조rs8636다태성위점적등위기인화기인형적빈수분포균부합Hardy-Weinberg평형검험(X~2=2.69,v=1,P＞0.05;X~2=2.52,v=1,P＞0.05).②환자조여대조조SNAP-25기인rs8636다태성위점적기인형화등위기인빈솔적총체분포차이유통계학의의(X~2=6.454,P＜0.05;X~2=7.033,P＜0.05);③환자조C/C기인형빈솔(65.0%)화C등위기인빈솔(79.7%)고우대조조,차이유통계학의의(X~2=5.848,P＜0.05;OR=1.50);환자조T/T기인형빈솔(5.7%)략저우대조조,차이무통계학의의(X~2=2.453,P＞0.05).결론 SNAP-25기인rs8636다태성위점여정신분렬증가능존재관련.C/C기인형화C등위기인가능시정신분렬증발병적위험인소.
Objective To investigate the relationship between single nucleotide polymorphism(rs8636)in synaptosomal-associated protein 25kDa gene(SNAP-25)and schizophrenia.Methods The genotypes and alleles in 300 patients with schizophrenia and 300 normal controls were examined with polymerase chain reaction and restriction fragment length polymorphism technique.Analyzed the association between schizophrenia and polymorphism of SNAP-25 gene.Results ①There was no deviation from Hardy-Weinberg disequilibrium for the frequency distribution of genotypes and alleles in the two groups(X~2=2.69，v=1，P＞0.05；X~2=2.52，v=1，P＞0.05).②There were significant differences in the frequencies of the genotypes and alleles between patients and
controls(X~2=6.454，P＜0.05；X~2=7.033，P＜0.05).③The frequency of C/C genotype(65.0%)and C alleles (79.7%)in patients were higher than that in the controls(X~2=5.848，P＜0.05；OR=1.50).The frequency of T/T genotype(5.7%)in patients was lower than that in the controls(X~2=2.453，P＞0.05).Conclusion There is a relationship between rs8636 in SNAP-25 gene and schizophrenia.The genotype C/C and alleles C may be a risk factor to the pathogenesis of schizophrenia.
