CAJ | 학술논문

目的观察转化生长因子(TGF)-β1及其Ⅰ型受体在两种大鼠肺动脉高压模型中表达的变化.方法 36只Spargue-Dawley大鼠,对照组、分流型和药物型肺动脉高压模型各12只,使用原位杂交方法观察TGF-β1及其Ⅰ型受体在肺小动脉表达的定位,并使用逆转录-聚合酶链反应(RT-PCR)法观察两者mRNA转录量的变化.结果大鼠分流型与药物型肺动脉高压模型中TGF-β1及其Ⅰ型受体mRNA主要分布于肺小动脉内皮细胞和外膜成纤维细胞,两种模型中TGF-β1(1.17±0.09、1.09±0.07)及其Ⅰ型受体(0.80±0.06、0.93±0.02)mRNA转录均较对照组明显增加,三组间均数比较差异有统计学意义(P0.05).结论 TGF-β1信号参与了肺动脉高压肺血管重塑的过程,但在不同的模型中作用机制不同.
목적 관찰전화생장인자(TGF)-β1급기Ⅰ형수체재량충대서폐동맥고압모형중표체적변화.방법 36지Spargue-Dawley대서,대조조、분류형화약물형폐동맥고압모형각12지,사용원위잡교방법관찰TGF-β1급기Ⅰ형수체재폐소동맥표체적정위,병사용역전록-취합매련반응(RT-PCR)법관찰량자mRNA전록량적변화.결과 대서분류형여약물형폐동맥고압모형중TGF-β1급기Ⅰ형수체mRNA주요분포우폐소동맥내피세포화외막성섬유세포,량충모형중TGF-β1(1.17±0.09、1.09±0.07)급기Ⅰ형수체(0.80±0.06、0.93±0.02)mRNA전록균교대조조명현증가,삼조간균수비교차이유통계학의의(P0.05).결론 TGF-β1신호삼여료폐동맥고압폐혈관중소적과정,단재불동적모형중작용궤제불동.
Objective To compare the different expression of TGF-β1 and TGF-βR1 in two types of pulmonary artery hypertension model of rats.Methods Thirty-six Spargue-Dawley rats were divided into 3 groups(control,carotid artery-jugular vein shunt pulmonary hypertension model and monocrotalin-induced pulmonary hypertension model).Each group had 12 rats.TGF-β1 and TGF-βR I localization was evaluated by in situ hybridization.and mRNA transcription was detected by RT-PCR.Results The localizations of TGF-β1 and TGF-βR I in two madels were endothelial cells and extima fibroblast cells of pulmonary arteries,respectively.The expression of TGF-β1(1.17±0.09,1.09±0.07)and TGF-βR I mRNA(0.80±0.06,0.93±0.02)was increased in both two models.There was significant difference in multiple comparison of three groups(P＜0.05).Conclusion TGF-β1 signaling is involved in pulmonary vascular remodeling of pulmonary hypertension process,and its mechanisms in two pulmonary artery hypertension models are different.