CAJ | 학술논문

目的建立尺神经前置的动物模型并从分子水平上评价尺神经前置的安全性.方法取健康成年SD大鼠20只,建立右前肢尺神经前置模型,左侧(非手术侧)作为自身对照,术后1个月处死大鼠,取双侧尺侧腕屈肌称重及下颈段脊髓(C_6～T_1)切片,通过Nissl染色、还原型辅酶Ⅱ-黄递酶(NADPH-d)组织化学染色、植物凝集素(IB4)染色、胆碱乙酰基转移酶(CHAT)免疫组织化学染色观察颈髓前角、后角神经元的形态,电镜观察颈髓前角ChAT免疫阳性运动神经元的超微结构.结果与对照侧[(93.2±7.29)mg]比较,大鼠尺神经前置侧尺侧腕屈肌质量[(92.3±9.13)mg]无明显变化,差异无统计学意义(t=0.910,P=0.378);Niss1染色、NADPH-d、IB4、ChAT免疫组织化学染色结果均显示大鼠尺神经前置侧和对照侧相比较细胞形态无明显改变,阳性细胞数量差异均无统计学意义(P>0.05);电镜结果显示颈髓前角ChAT免疫阳性运动神经元的超微结构无明显改变.结论动物实验基础上尺神经前置术是安全的.
목적 건립척신경전치적동물모형병종분자수평상평개척신경전치적안전성.방법 취건강성년SD대서20지,건립우전지척신경전치모형,좌측(비수술측)작위자신대조,술후1개월처사대서,취쌍측척측완굴기칭중급하경단척수(C_6～T_1)절편,통과Nissl염색、환원형보매Ⅱ-황체매(NADPH-d)조직화학염색、식물응집소(IB4)염색、담감을선기전이매(CHAT)면역조직화학염색관찰경수전각、후각신경원적형태,전경관찰경수전각ChAT면역양성운동신경원적초미결구.결과 여대조측[(93.2±7.29)mg]비교,대서척신경전치측척측완굴기질량[(92.3±9.13)mg]무명현변화,차이무통계학의의(t=0.910,P=0.378);Niss1염색、NADPH-d、IB4、ChAT면역조직화학염색결과균현시대서척신경전치측화대조측상비교세포형태무명현개변,양성세포수량차이균무통계학의의(P>0.05);전경결과현시경수전각ChAT면역양성운동신경원적초미결구무명현개변.결론 동물실험기출상척신경전치술시안전적.
Objective To establish animal models with anterior transposition of the ulnar nerve and evaluate the safety of anterior transposition of the ulnar nerve at molecular level.Methods Location of the ulnar nerve of elbow in 5 rats were found similar to human being by anatomy.Twenty healthy adult SD rats,weighting about 250 g,were performed the anterior transposition of the ulnar nerve in the right forelimbs and the left forelimbs was considered as control group.The bilateral flexor carpi ulnaris muscles were weighed and the slice of cervical spinal cord(C_6-T_1)level were prepared 1 month after the operation.Nissl staining,NADPH-d histochemical staining,IB4 staining and ChAT-immunohistochemical staining were employed to observe the spinal cord(C_6-T_1)level at molecular level;electron microscope was used to observe the ultrastructure of ChAT-positive neurons.Statistical analysis was paired T test.Results The flexor carpi ulnaris muscles in the model group(92.3±9.13mg)and control group(93.2±7.29 mg)were not significantly different(P>0.05).After anterior transposition of the ulnar nerve in rats,no significant differences in cell number and morphology in the cervical spinal cord(C_6-T_1)were found between the model group and the control group(P>0.05).No changes between the 2 groups were noted in the fine structure of anterior horn motor neurons and the expression of nenrotransmitters(P>0.05).Conclusion Anterior transposition of the ulnar nerve can be safely done in the animal models(rats).