中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
8期
1550-1552
,共3页
宋军%王辉%付海啸%孟松%徐溢新%刘浩%王磊%白津%徐为%郑骏年
宋軍%王輝%付海嘯%孟鬆%徐溢新%劉浩%王磊%白津%徐為%鄭駿年
송군%왕휘%부해소%맹송%서일신%류호%왕뢰%백진%서위%정준년
胃癌%miR-155%miR-30a
胃癌%miR-155%miR-30a
위암%miR-155%miR-30a
Gastric carcinoma%miR-155%miR-30a
目的 探讨胃癌组织和癌旁组织miR-155和miR-30a的表达差异及其临床意义.方法 收集手术切除的胃癌标本52例,采用实时荧光定量逆转录-聚合酶链反应( RT-qPCR)检测癌及癌旁组织标本miR-155和miR-30a的表达水平,分析其与临床病理的关系.结果 miR-155在胃癌组织中表达量(0.84 ±2.27)显著高于其在配对癌旁组织中的表达值(0.28 ±0.56,P＜0.05)；miR-155高表达与淋巴结转移有关(P＜0.05).miR-30a在胃癌组织中表达量(3.16±8.11)显著低于其在配对癌旁组织中的表达值(15.87±35.58,P＜0.05).miR-30a的低表达与肿瘤侵犯层次、淋巴结转移有关(P＜0.05).结论 miR-155和miR-30a与胃癌发生发展密切相关,可能成为胃癌的潜在诊断及治疗靶点.
目的 探討胃癌組織和癌徬組織miR-155和miR-30a的錶達差異及其臨床意義.方法 收集手術切除的胃癌標本52例,採用實時熒光定量逆轉錄-聚閤酶鏈反應( RT-qPCR)檢測癌及癌徬組織標本miR-155和miR-30a的錶達水平,分析其與臨床病理的關繫.結果 miR-155在胃癌組織中錶達量(0.84 ±2.27)顯著高于其在配對癌徬組織中的錶達值(0.28 ±0.56,P＜0.05)；miR-155高錶達與淋巴結轉移有關(P＜0.05).miR-30a在胃癌組織中錶達量(3.16±8.11)顯著低于其在配對癌徬組織中的錶達值(15.87±35.58,P＜0.05).miR-30a的低錶達與腫瘤侵犯層次、淋巴結轉移有關(P＜0.05).結論 miR-155和miR-30a與胃癌髮生髮展密切相關,可能成為胃癌的潛在診斷及治療靶點.
목적 탐토위암조직화암방조직miR-155화miR-30a적표체차이급기림상의의.방법 수집수술절제적위암표본52례,채용실시형광정량역전록-취합매련반응( RT-qPCR)검측암급암방조직표본miR-155화miR-30a적표체수평,분석기여림상병리적관계.결과 miR-155재위암조직중표체량(0.84 ±2.27)현저고우기재배대암방조직중적표체치(0.28 ±0.56,P＜0.05)；miR-155고표체여림파결전이유관(P＜0.05).miR-30a재위암조직중표체량(3.16±8.11)현저저우기재배대암방조직중적표체치(15.87±35.58,P＜0.05).miR-30a적저표체여종류침범층차、림파결전이유관(P＜0.05).결론 miR-155화miR-30a여위암발생발전밀절상관,가능성위위암적잠재진단급치료파점.
Objective To investigate the expression of miR-155 and miR-30a in gastric carcinoma and the clinical significance.Methods Fifty-two pairs of human gastric carcinoma tissue samples were obtained from patients who underwent surgical resection.The level of miR-155 and miR-30a in 52 gastric carcinoma and the adjacent normal tissues was quantified by real-time fluorescent quantitative reverse transcriptase-polymerase chain reaction ( RT-qPCR),and the relationship between miR-155 and miR-30a level with clinicopathological factors was explored.Results The expression of miR-155 in gastric carcinoma tissues (0.84 ± 2.27) was significantly increased as compared with that in the adjacent normal tissues (0.28 ± 0.56,P ＜ 0.05 ).The up-regulated expression of miR-155 was associated with lymph node metastases in gastric carcinoma ( P ＜ 0.05 ).The expression of miR-30a in gastric cancer tissues (3.16 ± 8.11 )was significantly lower than that in the adjacent normal tissues ( 15.87 ± 35.58,P ＜ 0.05 ). The low expression of miR-30a was associated with lymph node metastases and local invasion in gastric carcinoma ( P ＜0.05).Conclusion miR-155 and miR-30a may play important roles in the development and progression of gastric carcinoma,and they may be potential biomarkers in the diagnosis and treatment of gastric carcinoma.
