中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2010年
6期
535-538
,共4页
宾萍%冷曙光%程娟%潘祖飞%段化伟%戴宇飞%李海山%牛勇%刘清君%刘庆%郑玉新
賓萍%冷曙光%程娟%潘祖飛%段化偉%戴宇飛%李海山%牛勇%劉清君%劉慶%鄭玉新
빈평%랭서광%정연%반조비%단화위%대우비%리해산%우용%류청군%류경%정옥신
职业暴露%焦炭%烃类%芳香%端粒
職業暴露%焦炭%烴類%芳香%耑粒
직업폭로%초탄%경류%방향%단립
Occupational exposure%Coke%Hydrocarbons%Aromatic%Telomere
目的 探讨多环芳烃(PAHs)暴露与端粒长度的关系,以寻找评价多环芳烃致外周血遗传物质损伤的效应性生物标志物.方法 以145名焦炉作业工人(炉顶工30名、炉侧工76名、炉底工39名)作为暴露组,68名医护人员作为对照组,收集其工作周末班6 h后的尿液和1 ml肘静脉血.测定研究对象工作环境空气中苯溶物和颗粒相苯并(a)芘浓度、尿中1-羟基芘(1-OHPyr)水平;应用定量PCR方法测定其外周血全基因组DNA的相对端粒长度(RTL),分析端粒长度与PAHs外暴露水平和相关影响因素的关系.结果 暴露组空气中苯溶物和颗粒相苯并(a)芘浓度的中位数分别为328.6 μg/m3和926.9 ng/m3,均高于对照组区域[苯溶物为97.8 μg/m3,颗粒相苯并(a)芘浓度为49.1 ng/m3].暴露组尿1-OHPyr水平(12.2 μmol/mol Cr)高于非暴露组(0.7 μmol/mol Cr),差异有统计学意义(t=26.971,P<0.01).暴露组RTL为1.10 ±0.75,短于非暴露组的1.43±1.06,差异有统计学意义(t=2.263,P=0.026),校正每日吸烟量和尿1-OHPyr水平后的协方差分析也显示差异有统计学意义(F校正=5.496,P校正=0.020).分层分析中发现PAHs暴露对RTL的影响在男性(暴露组:1.08 ±0.73;非暴露组:1.51±1.10;F=9.212,P=0.003)和不饮酒(暴露组:0.96±0.38;非暴露组:1.26±0.46;F=6.484,P=0.012)的工人中更为显著.非暴露组的RTL与年龄呈负相关(r=-0.284,P=0.019).结论 PAHs暴露可对工人外周血全基因组DNA的端粒长度产生影响,这种影响在男性和不饮酒的焦炉工中更为明显,提示端粒长度可能是PAHs暴露致遗传物质损伤的效应生物标志物之一.
目的 探討多環芳烴(PAHs)暴露與耑粒長度的關繫,以尋找評價多環芳烴緻外週血遺傳物質損傷的效應性生物標誌物.方法 以145名焦爐作業工人(爐頂工30名、爐側工76名、爐底工39名)作為暴露組,68名醫護人員作為對照組,收集其工作週末班6 h後的尿液和1 ml肘靜脈血.測定研究對象工作環境空氣中苯溶物和顆粒相苯併(a)芘濃度、尿中1-羥基芘(1-OHPyr)水平;應用定量PCR方法測定其外週血全基因組DNA的相對耑粒長度(RTL),分析耑粒長度與PAHs外暴露水平和相關影響因素的關繫.結果 暴露組空氣中苯溶物和顆粒相苯併(a)芘濃度的中位數分彆為328.6 μg/m3和926.9 ng/m3,均高于對照組區域[苯溶物為97.8 μg/m3,顆粒相苯併(a)芘濃度為49.1 ng/m3].暴露組尿1-OHPyr水平(12.2 μmol/mol Cr)高于非暴露組(0.7 μmol/mol Cr),差異有統計學意義(t=26.971,P<0.01).暴露組RTL為1.10 ±0.75,短于非暴露組的1.43±1.06,差異有統計學意義(t=2.263,P=0.026),校正每日吸煙量和尿1-OHPyr水平後的協方差分析也顯示差異有統計學意義(F校正=5.496,P校正=0.020).分層分析中髮現PAHs暴露對RTL的影響在男性(暴露組:1.08 ±0.73;非暴露組:1.51±1.10;F=9.212,P=0.003)和不飲酒(暴露組:0.96±0.38;非暴露組:1.26±0.46;F=6.484,P=0.012)的工人中更為顯著.非暴露組的RTL與年齡呈負相關(r=-0.284,P=0.019).結論 PAHs暴露可對工人外週血全基因組DNA的耑粒長度產生影響,這種影響在男性和不飲酒的焦爐工中更為明顯,提示耑粒長度可能是PAHs暴露緻遺傳物質損傷的效應生物標誌物之一.
목적 탐토다배방경(PAHs)폭로여단립장도적관계,이심조평개다배방경치외주혈유전물질손상적효응성생물표지물.방법 이145명초로작업공인(로정공30명、로측공76명、로저공39명)작위폭로조,68명의호인원작위대조조,수집기공작주말반6 h후적뇨액화1 ml주정맥혈.측정연구대상공작배경공기중분용물화과립상분병(a)비농도、뇨중1-간기비(1-OHPyr)수평;응용정량PCR방법측정기외주혈전기인조DNA적상대단립장도(RTL),분석단립장도여PAHs외폭로수평화상관영향인소적관계.결과 폭로조공기중분용물화과립상분병(a)비농도적중위수분별위328.6 μg/m3화926.9 ng/m3,균고우대조조구역[분용물위97.8 μg/m3,과립상분병(a)비농도위49.1 ng/m3].폭로조뇨1-OHPyr수평(12.2 μmol/mol Cr)고우비폭로조(0.7 μmol/mol Cr),차이유통계학의의(t=26.971,P<0.01).폭로조RTL위1.10 ±0.75,단우비폭로조적1.43±1.06,차이유통계학의의(t=2.263,P=0.026),교정매일흡연량화뇨1-OHPyr수평후적협방차분석야현시차이유통계학의의(F교정=5.496,P교정=0.020).분층분석중발현PAHs폭로대RTL적영향재남성(폭로조:1.08 ±0.73;비폭로조:1.51±1.10;F=9.212,P=0.003)화불음주(폭로조:0.96±0.38;비폭로조:1.26±0.46;F=6.484,P=0.012)적공인중경위현저.비폭로조적RTL여년령정부상관(r=-0.284,P=0.019).결론 PAHs폭로가대공인외주혈전기인조DNA적단립장도산생영향,저충영향재남성화불음주적초로공중경위명현,제시단립장도가능시PAHs폭로치유전물질손상적효응생물표지물지일.
Objective To explore the association between polyeyclic aromatic hydrocarbons (PAHs) exposure and telomere length(TL),so as to investigate the effective biomarkers to evaluate the genetic daolage in peripheral blood of workers exposed to PAHs.Methods The exposure group consisted of 145 coke-oven workers (including 30 top-oven workers,76 side-oven workers and 39 bottom-oven workers),and the non-exposure control group comprised 68 medical staffs.At 6 hours after the weekend duty shift,the samples of urine and 1 ml venous blood were collected from each subject. Airborne benzene-soluble matter (BSM)and particulate-phase B(a)P in the working environment of coke-oven and controls were sampled and analyzed.The concentration of urinary 1-hydroxypyrene(1-OHPyr)was determined.A real-time PCR method was used to determine the relative telomere length(RTL)of genomic DNA in peripheral blood.The relationship between the RTL and external exposure of PAHs,the potential factors which might have influence on TL were analyzed.Results The medians of air BSM and particulate-phase B(a)P were higher in cokeoven(BSM:328.6 μg/m3;B(a)P:926.9 ng/m3)than those in control working environment(BSM:97.8 μg/m3;B(a)P:49.1 ng/m3).The level of 1-OHPyr among coke-oven workers was significantly higher than that of non-exposed group ( 12.2 μmol/mol Cr vs 0. 7 μmol/mol Cr; t = 26. 971, P < 0. 01 ). RTL in coke-oven workers were significantly shorter than those of controls ( 1.10 ±0. 75 vs 1.43 ± 1.06; t =2. 263,P =0. 026) ,and after adjusting for cigarettes per day and urinary 1-OHPyr,the significant difference was still observed ( Fadju = 5.496, Padju = 0. 020). Stratification analysis found that RTL among the male and nondrinking groups in coke-oven workers were shorter than those the same sex and alcohol using status in controls ( 1. 08± 0. 73 vs 1. 51± 1.10, F = 9. 212, P = 0. 003 ; 0. 96 ± 0. 38 vs 1. 26 ± 0.46, F = 6. 484, P =0. 012 ). Significant correlation between RTL and age was found ( r = -0. 284, P =0. 019 ) in non-exposure group. Conclusion PAH-expesure has effect on TL of genomic DNA in peripheral blood,which is mainly observed in the male and non-drinking groups between PAH-exposed workers and controls. It indicates that TL of gcnomic DNA in peripheral blood might be an effective biomarker as PAH-induced genetic damage.
