中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
12期
1110-1113
,共4页
李广学%修冰水%凌世淦%张贺秋%闫涛%魏林%贺峰%冯晓燕%宋晓国%王国华%陈坤%何竟%曾瑞红
李廣學%脩冰水%凌世淦%張賀鞦%閆濤%魏林%賀峰%馮曉燕%宋曉國%王國華%陳坤%何竟%曾瑞紅
리엄학%수빙수%릉세감%장하추%염도%위림%하봉%풍효연%송효국%왕국화%진곤%하경%증서홍
丙型肝炎病毒%重组蛋白联合疫苗(TFE)%佐剂%CpG%Al(OH)3
丙型肝炎病毒%重組蛋白聯閤疫苗(TFE)%佐劑%CpG%Al(OH)3
병형간염병독%중조단백연합역묘(TFE)%좌제%CpG%Al(OH)3
Hepatitis C virus%Recombinant protein combined vaccine(TFE)%Adjuvant%CpG%Al(OH)3
目的 CpG和Al(OH)3佐剂联合使用增强丙型肝炎病毒(HCV)重组蛋白联合疫苗(TFE)的细胞免疫原性.方法 以CpG、Al(OH)3、Al(OH)3+CpG和弗氏佐剂(FA)为免疫佐剂,分别与TFE混合免疫BALB/c小鼠.末次免疫后10 d取静脉血,分离血清,用ELISA方法测定血清中特异性抗体,并每组处死5只小鼠,分离小鼠脾细胞,体外检测IFN-γ、IL-4和CTL杀伤试验;剩余的小鼠背部皮下注射1×106个SP2/O-NS3细胞,观察其保护作用.组间均数差异用LSD-t检验.结果 TFE+Al(OH)3+CpG组的特异性CTL杀伤能力高于TFE+FA组和TFE+CpG组(P<0.05);与TFE+Al(OH)3组和TFE+CpG组相比,TFE+Al(OH)3+CpG组小鼠脾细胞中分泌IFN-γ的淋巴细胞的数量显著增多(P<0.05).结论 Al(OH)3和CpG合用能显著增强HCV重组蛋白联合疫苗TFE的细胞免疫原性;TFE+Al(OH)3+CpG能有效预防表达HCV非结构蛋白NS3的细胞SP2/0-NS3对免疫小鼠的攻击.
目的 CpG和Al(OH)3佐劑聯閤使用增彊丙型肝炎病毒(HCV)重組蛋白聯閤疫苗(TFE)的細胞免疫原性.方法 以CpG、Al(OH)3、Al(OH)3+CpG和弗氏佐劑(FA)為免疫佐劑,分彆與TFE混閤免疫BALB/c小鼠.末次免疫後10 d取靜脈血,分離血清,用ELISA方法測定血清中特異性抗體,併每組處死5隻小鼠,分離小鼠脾細胞,體外檢測IFN-γ、IL-4和CTL殺傷試驗;剩餘的小鼠揹部皮下註射1×106箇SP2/O-NS3細胞,觀察其保護作用.組間均數差異用LSD-t檢驗.結果 TFE+Al(OH)3+CpG組的特異性CTL殺傷能力高于TFE+FA組和TFE+CpG組(P<0.05);與TFE+Al(OH)3組和TFE+CpG組相比,TFE+Al(OH)3+CpG組小鼠脾細胞中分泌IFN-γ的淋巴細胞的數量顯著增多(P<0.05).結論 Al(OH)3和CpG閤用能顯著增彊HCV重組蛋白聯閤疫苗TFE的細胞免疫原性;TFE+Al(OH)3+CpG能有效預防錶達HCV非結構蛋白NS3的細胞SP2/0-NS3對免疫小鼠的攻擊.
목적 CpG화Al(OH)3좌제연합사용증강병형간염병독(HCV)중조단백연합역묘(TFE)적세포면역원성.방법 이CpG、Al(OH)3、Al(OH)3+CpG화불씨좌제(FA)위면역좌제,분별여TFE혼합면역BALB/c소서.말차면역후10 d취정맥혈,분리혈청,용ELISA방법측정혈청중특이성항체,병매조처사5지소서,분리소서비세포,체외검측IFN-γ、IL-4화CTL살상시험;잉여적소서배부피하주사1×106개SP2/O-NS3세포,관찰기보호작용.조간균수차이용LSD-t검험.결과 TFE+Al(OH)3+CpG조적특이성CTL살상능력고우TFE+FA조화TFE+CpG조(P<0.05);여TFE+Al(OH)3조화TFE+CpG조상비,TFE+Al(OH)3+CpG조소서비세포중분비IFN-γ적림파세포적수량현저증다(P<0.05).결론 Al(OH)3화CpG합용능현저증강HCV중조단백연합역묘TFE적세포면역원성;TFE+Al(OH)3+CpG능유효예방표체HCV비결구단백NS3적세포SP2/0-NS3대면역소서적공격.
Objective To research CpG and Al(OH)3 adjuvants enhancing immunogenicity of hepatitis C virus(HCV) recombinant ptotein combined vaccine(TIE).Methods BALB/c mice were immunized with candidate vaccine TFE using CpG,Al(OH)3,Al(OH) 3 + CpG,or freund's adjuvant(FA) as the adjuvant.Five mice were sacrificed after 10 d of the last immunization.Specific antibodies in sera were tested by enzyme-linked immunosorbent assay(ELISA).Splenic cells were isolated and levels of IFN-γ,IL-4 and cytotoxic T lymphocyte(CTL) cytotoxicity assay were messuredin vitro.The remaining mice were subcutaneouly injected with 1 × 106 SP2/0-NS3 cells on the back to investigate the protective effects.The differences of means between groups were compared by LSD-t test.Results The specific CTL activity of TFE + A1(OH) 3 + CpG group was higher than TFE + FA group and TFE + CpG group(P ＜ 0.05).The level of IFN-γsecreting cells in TFE + Al(OH)3 + CpG group was higher than that in TFE + M(OH)3 group or TFE + CpG group(P ＜ 0.05).Conclusion Combining Al(OH) 3 and CpG could enhance specific cellular immunogenicity of candidate HCV vaccine TFE.TFE + M(OH) 3 + CpG could effetively prevent the attack of tumor cell SP2/0-NS3 expressing nonstructural protein NS3 of HCV.
