CAJ | 학술논문

目的了解大环内酯类耐药基因在人源和动物源性肠球菌之间水平转移的可能性. 方法采用KB法和琼脂稀释法检测52株动物源性和55株儿科临床分离肠球菌对8种抗生素的敏感性;PCR检测肠球菌的ermB、mefA、tetM、aac(6')/aph(2")基因以及Tn1545的整合酶int基因;PCR扩增联合测序对比人源性和动物源性ermB等位基因的同源性;接合实验研究ermB基因在不同种属、不同来源的肠球菌之间水平转移. 结果两种来源肠球菌对红霉素的耐药率高达80%以上;ermB是肠球菌对红霉素耐药的主要基因,在人源性和动物源性肠球菌的检出率分别为61.82%(34/55)和53.85%(28/52);已测序菌株中53.0%的北京临床分离株与69.5%的动物分离株携带同一ermB等位基因EF525477;通过接合实验,ermB基因可以在不同来源、不同种属分离株之间进行传递. 结论动物源性和人源性肠球菌对红霉素和四环素都有高耐药率;滤膜实验证实ermB基因在体外条件下可以在动物源性肠球菌和人源性肠球菌之间传递,但传递频率比较低.动物源性和人源性肠球菌及不同种属的肠球菌有相同ermB等位基因,提示其ermB耐药基因在动物源性和人源性肠球菌水平传递存在的可能性.
목적 료해대배내지류내약기인재인원화동물원성장구균지간수평전이적가능성. 방법 채용KB법화경지희석법검측52주동물원성화55주인과림상분리장구균대8충항생소적민감성;PCR검측장구균적ermB、mefA、tetM、aac(6')/aph(2")기인이급Tn1545적정합매int기인;PCR확증연합측서대비인원성화동물원성ermB등위기인적동원성;접합실험연구ermB기인재불동충속、불동래원적장구균지간수평전이. 결과 량충래원장구균대홍매소적내약솔고체80%이상;ermB시장구균대홍매소내약적주요기인,재인원성화동물원성장구균적검출솔분별위61.82%(34/55)화53.85%(28/52);이측서균주중53.0%적북경림상분리주여69.5%적동물분리주휴대동일ermB등위기인EF525477;통과접합실험,ermB기인가이재불동래원、불동충속분리주지간진행전체. 결론 동물원성화인원성장구균대홍매소화사배소도유고내약솔;려막실험증실ermB기인재체외조건하가이재동물원성장구균화인원성장구균지간전체,단전체빈솔비교저.동물원성화인원성장구균급불동충속적장구균유상동ermB등위기인,제시기ermB내약기인재동물원성화인원성장구균수평전체존재적가능성.
Objective To obtain better insights into transmission dynamics of macrolide resistance genes between human and animal Enterococcus strains.Methods The antimicrobial susceptibility to 8 anti-bioties of 52 Enterococci isolated from animal and 55 Enterococci isolated from human was determined.PCR was used to detect the macrolide resistance genes ermB and mefA,tetracycline resistance genes tetM,and the integrase gene int of Tnl545 of the total 107 strains.Forty-nine ermB positive strains were chosen to be se-quenced.Filter mating experiments were taken.Results The resistance rate to erythromycin were 89.09% and 80.77%for isolates from human and animal:and resistance rate to tetracycline were 80.00%and 67.3l%for isolates from human and animal.respectively.All isolated Enterococci strains were found sensi-tive to vancomycin ermB was detected in 61.82% human Enterococci and 53.85% porcine ones.Identical er-mB gene sequences were found in animal and human Enterococci.Transfer of the ermB gene from porcine E.faecalis to human E.feacalis was successful.and the transfer frequency is 1.2×10-5.Conclusion En-terococci have a high resistance rate to erythromycin and some other antibio tics,especially in pediatric iso-lates:but still very sensitive to glycopeptide.ermB was the predominant genes for macrolide and tetracy-cline.Identical ermB gene sequences were present in animal and human Enterococci and that transfer of the ermB gene from porcine E.faecalis to human E.faecalis and vice versa is possible.but probably occurs at a low frequency.