CAJ | 학술논문

目的研究重组腺病毒Ad-hTERTp-HSV-TK/GCV系统对小鼠肝癌腹腔种植腹腔积液生成的影响及其作用机制.方法 sx_1,近交系小鼠腹腔内接种肝癌H22细胞株后,随机分为四组,48 h后分别给予相应的治疗.观察各组小鼠腹腔积液及生存时间的变化;流式细胞术测定肿瘤细胞凋亡百分率;透射电镜观察腹腔内肿瘤细胞的形态学变化.结果与Ad-hTERTp-HSV-TK组、GCV组和空白对照组三组相比,Ad-hTERTp-HSV-TK/GCV组可以显著抑制小鼠腹腔积液的生成(P<0.01),延长生存期(P<0.01),肿瘤细胞凋亡率为(27.12±2.12)%明显高于其他各组(P<0.01),并且在治疗期间未发现明显不良反应.结论 Ad-hTERTp-HSV-TK/GCV系统可通过诱导肝癌细胞凋亡而抑制小鼠腹腔积液生成,并延长生存期,是一种安全可行的治疗方法.
목적 연구중조선병독Ad-hTERTp-HSV-TK/GCV계통대소서간암복강충식복강적액생성적영향급기작용궤제.방법 sx_1,근교계소서복강내접충간암H22세포주후,수궤분위사조,48 h후분별급여상응적치료.관찰각조소서복강적액급생존시간적변화;류식세포술측정종류세포조망백분솔;투사전경관찰복강내종류세포적형태학변화.결과 여Ad-hTERTp-HSV-TK조、GCV조화공백대조조삼조상비,Ad-hTERTp-HSV-TK/GCV조가이현저억제소서복강적액적생성(P<0.01),연장생존기(P<0.01),종류세포조망솔위(27.12±2.12)%명현고우기타각조(P<0.01),병차재치료기간미발현명현불량반응.결론 Ad-hTERTp-HSV-TK/GCV계통가통과유도간암세포조망이억제소서복강적액생성,병연장생존기,시일충안전가행적치료방법.
Objective To observe the effect of Ad-bTERTp-HSV-TK/GCV system on malignant ascites of mice and probe into its mechanism of action.Methods The SX1 inbred strain mice were injected with H22 cell line of liver cancer and were divided into 4 groups at random.The mice in each group were given corresponding treatment after 48 hours.The production of ascites and survival period were evaluated. The apoptosis rates of tumor cells were detected by FCM.Morphological changes of tumor cells were studied by electromicroscope.Results Compared with other groups.Ad-hTERTp-HSV-TK/GCV Can obviously inhibit the production of ascites(P<0.01),prolong the survival period (P<0.01),and apoptosis rate in this group (27.12±2.12)% was significantly higher than that in other groups.No obvious side effect Was found during the treatment.Conclusion Ad-hTERTp-HSV-TK/GCV system Can inhibit production of ascites and prolong the survical period of mice by inducing apoptosis of hepatoma cells,which is a safe and feasible treatment for hepatoma therapy.