CAJ | 학술논문

涅斯捷连科氏菌属的建立基于对Micrococcus halobius的再分类,这是一类广泛分布于高盐土壤环境的革兰氏阳性细菌,属放线菌类.在对我国西部盐湖环境放线菌的生物多样性及分类学研究中,大量类似菌株被分离.[目的]为了对其进行快速鉴定,特别是筛选出属于优势类群的涅斯捷连科氏菌,[方法]本研究根据前人以报道的方法设计了一对针对其16S rRNA基因的特异性PCR引物(Nes1/Nes2),[结果]并通过部分典型菌和野生菌株的PCR实验验证,结合16S rRNA基因的测序验证,证明了Nes1/Nes2的PCR反应有效性及其对涅斯捷连科氏菌的特异性.[结论]利用该引物可以快速准确的对涅斯捷连科氏菌进行鉴定.
열사첩련과씨균속적건립기우대Micrococcus halobius적재분류,저시일류엄범분포우고염토양배경적혁란씨양성세균,속방선균류.재대아국서부염호배경방선균적생물다양성급분류학연구중,대량유사균주피분리.[목적]위료대기진행쾌속감정,특별시사선출속우우세류군적열사첩련과씨균,[방법]본연구근거전인이보도적방법설계료일대침대기16S rRNA기인적특이성PCR인물(Nes1/Nes2),[결과]병통과부분전형균화야생균주적PCR실험험증,결합16S rRNA기인적측서험증,증명료Nes1/Nes2적PCR반응유효성급기대열사첩련과씨균적특이성.[결론]이용해인물가이쾌속준학적대열사첩련과씨균진행감정.
[Objective and Method]: For rapid identification of Nesterenkonia species, one set of genus-specific primers was designed and synthesized for polymerase chain reaction based on the 16S rRNA gene sequences.[Results and Conclusion]: The genus specificity of these primers was validated with reference strains as well as with wild-type isolates. Partial sequencing results of 16S rRNA gene of the wild-type isolates confirmed that they are members of the genus Nesterenkonia.