CAJ | 학술논문

建立红果樫木树皮中罗希吐碱的提取工艺.利用L9(34)正交实验确定罗希吐碱的提取条件,比较5种填料(XAD-2树脂,聚酰胺,葡聚糖凝胶LH-20,ODS和阳离子交换树脂)以及液液萃取技术对红果樫木提取物中罗希吐碱的分离效果.提取条件确定为:药材用70%7,醇(v/m=60)超声提取60分钟;提取物溶解于酸水溶液(0.5 mol/L HCl调pH 1),经等体积正丁醇萃取除去杂质;25%氨水调剩余水溶液pH 10,再经等体积正丁醇萃取;正丁醇萃取物溶解于酸水溶液(0.5 mol/L HCI调pH 1),经阳离子交换树脂色谱分离,水和70%乙醇(pH 10,25%氨水调节)为洗脱剂.罗希吐碱存在于70%Z,醇洗脱物中,含量可达到53.3%.该工艺为从红果樫木树皮中制备罗希吐碱提取物提供一种简单有效的方法.
건립홍과견목수피중라희토감적제취공예.이용L9(34)정교실험학정라희토감적제취조건,비교5충전료(XAD-2수지,취선알,포취당응효LH-20,ODS화양리자교환수지)이급액액췌취기술대홍과견목제취물중라희토감적분리효과.제취조건학정위:약재용70%7,순(v/m=60)초성제취60분종;제취물용해우산수용액(0.5 mol/L HCl조pH 1),경등체적정정순췌취제거잡질;25%안수조잉여수용액pH 10,재경등체적정정순췌취;정정순췌취물용해우산수용액(0.5 mol/L HCI조pH 1),경양리자교환수지색보분리,수화70%을순(pH 10,25%안수조절)위세탈제.라희토감존재우70%Z,순세탈물중,함량가체도53.3%.해공예위종홍과견목수피중제비라희토감제취물제공일충간단유효적방법.
To develop a simple and rapid purification method of rohitukine from the stem bark of Dysoxylum binectariferum. A L9(34) orthogonal test was designed to optimize the extraction condition. Rohitukine in the plant extract was purified by using solvent-solvent partition and cation exchange resion (CER). Five different types of packing materials, including XAD-2 resin, polyamide, Sephadex LH-20, ODS and CER, were compared and CER showed the best capacity for rohitukine separation. The purification proce- dure was optimized as follows: the plant material powder was extracted with 70% ethanol (v/m = 60) by ultrasonic agitation for 60 rain, then the 70% ethanol extract was dissolved in aqueous solution (pH 1, adjusted with 0.5 mol/L HCl) and extracted with equal volume of n-butanol. The aqueous layer was retained and the pH was adjusted to 10 with 25% aqueous ammonia and a solvent- solvent partition was performed with equal volume of n-butanol. The obtained n-butanol extract was dissolved in aqueous solution (pH 1, adjusted with 0.5 mol/L HCl), and purified by a CER column eluting with H2O and 70% ethanol (pH 10, adjusted with 25% aqueous ammonia), successively. Rohitukine existed in 70% ethanol eluate, with a purity up to 53.3%. The method developed in this study provides a simple and rapid approach for the preparation of rohitukine from the stem bark ofD. binectariferum.