CAJ | 학술논문

胼胝质的合成和降解是雄配子体减数分裂过程中的一个重要特征,对后期花粉成熟有重要作用.在此研究中,分离到了一个雄性不育突变体msl57,该突变体的绒毡层分化及胼胝质降解过程出现异常,导致花粉败育.图位克隆和遗传分析表明:MSl57基因与bHLI-I家族转录因子DYTI(At4g21330)是同一基因.因此,将ms157突变体改名为dyt1-2.反式激活作用实验揭示了DYTI的激活功能域位于基因的250 ～504bp之间.通过酵母双杂交实验发现DYT1蛋白在体内可以形成同源二聚体来执行其功能.RT-PCR及定量PCR分析表明胼胝质酶相关基因A6的表达在突变体背景下严重下调.因此,DVF1通过调控胼胝质的降解来影响花药发育过程.
변지질적합성화강해시웅배자체감수분렬과정중적일개중요특정,대후기화분성숙유중요작용.재차연구중,분리도료일개웅성불육돌변체msl57,해돌변체적융전층분화급변지질강해과정출현이상,도치화분패육.도위극륭화유전분석표명:MSl57기인여bHLI-I가족전록인자DYTI(At4g21330)시동일기인.인차,장ms157돌변체개명위dyt1-2.반식격활작용실험게시료DYTI적격활공능역위우기인적250 ～504bp지간.통과효모쌍잡교실험발현DYT1단백재체내가이형성동원이취체래집행기공능.RT-PCR급정량PCR분석표명변지질매상관기인A6적표체재돌변체배경하엄중하조.인차,DVF1통과조공변지질적강해래영향화약발육과정.
Callose formation and dissolution during and after meiotic division is a unique feature of male meiosis and is important for pollen development in plants. Here, we report the identification and characterization of a male - sterile mutant, ms157, which exhibits irregular tapetum differentiation and callose dissolution during anther development in Arabidopsis. Molecular mapping and genetic analysis indicated that MS157 is an allele of bHLH transcription factor DYT1 (At4g21330). Thus, the mutant was renamed as dytl-2. Our trans -activation activity assay showed the activation domain of DYT1 is located in 250bp to 504bp. Yeast two hybrid assay also suggested that two DYTI entire proteius could form homedimers in vivo. RT - PCR and Real - Time PCR analysis indicated that caUase complex related gene A6 was down - regulated in the mutant background. Hence, DYT1 plays an important role in anther development by regulating callose dissolution.