CAJ | 학술논문

为了解马立克氏病病毒(MDV)强、弱毒株主要致肿瘤相关基因变异情况,本研究根据MDV GA株基因组序列,设计合成扩增基因组重复区的引物,得到MDV 814疫苗株病毒基因组中约26 kb的序列片段.与GenBank登录的强、弱毒株进行比较分析表明,扩增的MDV1型814疫苗株的长重复区为12 774 bp,预测的开放阅读框(ORF)有48个;短重复区为11 426 bp,预测的ORF有38个.发现了4个MDV 814株特有的ORF.814株在编码Meq、RLORF6和23 ku的重叠基因内具有类似于疫苗株CVI988的177 bp的插入;在RLORF12基因编码区内存在69 bp的缺失,该缺失位于病毒复制起始位点内.同时,发现7个814疫苗株特有的氨基酸突变,分布在6个ORF内.单核苷酸多态性(SNP)的鉴定发现,多个基因具有单核苷酸的突变,主要分布于Meq基因,其中氨基酸A115V(丙氨酸-缬氨酸),N142D(天冬酰胺-天门冬氨酸)的变异是814疫苗株所特有的.MDV 814疫苗株重复区的基因序列的比较分析将有助于MDV致肿瘤机制的研究.
위료해마립극씨병병독(MDV)강、약독주주요치종류상관기인변이정황,본연구근거MDV GA주기인조서렬,설계합성확증기인조중복구적인물,득도MDV 814역묘주병독기인조중약26 kb적서렬편단.여GenBank등록적강、약독주진행비교분석표명,확증적MDV1형814역묘주적장중복구위12 774 bp,예측적개방열독광(ORF)유48개;단중복구위11 426 bp,예측적ORF유38개.발현료4개MDV 814주특유적ORF.814주재편마Meq、RLORF6화23 ku적중첩기인내구유유사우역묘주CVI988적177 bp적삽입;재RLORF12기인편마구내존재69 bp적결실,해결실위우병독복제기시위점내.동시,발현7개814역묘주특유적안기산돌변,분포재6개ORF내.단핵감산다태성(SNP)적감정발현,다개기인구유단핵감산적돌변,주요분포우Meq기인,기중안기산A115V(병안산-힐안산),N142D(천동선알-천문동안산)적변이시814역묘주소특유적.MDV 814역묘주중복구적기인서렬적비교분석장유조우MDV치종류궤제적연구.
In order to understand genetic variation of tumor-related genes in virulent and attenuated strains of Marek's disease virus (MDV), a 26 kb sequence of repeat region was amplified from MDV1-type vaccine strain 814 genome using primers designed according to the genomic sequence of MDV GA strain. The long repeat region of MDV strain 814 was 12 774 bp in length with 48 predicted ORFs and the short repeat region contained 11 426 bp and 38 ORFs. Compared to the published sequences of virulent and attenuated MDV strains, the MDV 814 strain contained four unique ORFs, a 177 bp insertion in the overlapping genes that encode the Meq, RLORF6 and 23 ku proteins (which was also presented in vaccine strain CVI988) and a 69 bp deletion in the RLORF12 gene located in the replication origin (Ori) of MDV. There were seven amino acid mutations in six ORFs. Several single nucleotide polymorphisms (SNPs) were also identified in those ORFs. The mutations were mainly distributed in the Meq gene at amino acid position 115 A to V and 142 N to D.