中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2011年
1期
29-32
,共4页
朱融和%庄强%蔡芳芳%俞康%江松福
硃融和%莊彊%蔡芳芳%俞康%江鬆福
주융화%장강%채방방%유강%강송복
全身照射(TBI)%肠道通透性%肠三叶因子(ITF)
全身照射(TBI)%腸道通透性%腸三葉因子(ITF)
전신조사(TBI)%장도통투성%장삼협인자(ITF)
Total body irradiation (TBI)%Intestinal permeability%Intestinal trefoil factor(ITF)
目的 探讨全身照射(TBI)对肠道黏膜通透性和肠三叶因子(ITF)mRNA表达的影响以及二者之间的关系.方法 32只BALB/c小鼠随机均分为4组:空白对照组和总剂量8.0 Gy全身照射后4、8和12 d组.TBI剂量8.0 Gy,剂量率1.0 Gy/min.高效液相色谱-蒸发光散射检测器分析(HPLC-ELSD)检测尿液标本中乳果糖与甘露醇的排出率比值(L/M),评价各组小鼠的肠道通透性;收集空肠组织标本,实时荧光定量PCR检测肠道ITF mRNA表达水平.结果 TBI后4、8和12d组L/M比值分别为0.5092±0.0353,0.7174±0.0116和0.7295±0.0063,均明显高于空白对照组(0.2908±0.0533,F=321.47,P＜0.05).TBI后4、8和12 d组ITF mRNA表达水平分别为0.78612±0.1428,0.2521±0.1223,和0.2306±0.0221,均显著低于空白对照组(1.3498±0.0476,F=235.71,P＜0.05).各TBI组中L/M与肠道ITF mRNA水平呈显著负相关(r=-0.985,P＜0.01).结论 TBI后随着时间延长ITF mRNA表达逐渐下降而肠黏膜通透性逐渐增加,即TBI后ITF mRNA表达与肠道通透性呈显著负相关.ITF在TBI所致肠道黏膜通透性增加中起着保护作用.
目的 探討全身照射(TBI)對腸道黏膜通透性和腸三葉因子(ITF)mRNA錶達的影響以及二者之間的關繫.方法 32隻BALB/c小鼠隨機均分為4組:空白對照組和總劑量8.0 Gy全身照射後4、8和12 d組.TBI劑量8.0 Gy,劑量率1.0 Gy/min.高效液相色譜-蒸髮光散射檢測器分析(HPLC-ELSD)檢測尿液標本中乳果糖與甘露醇的排齣率比值(L/M),評價各組小鼠的腸道通透性;收集空腸組織標本,實時熒光定量PCR檢測腸道ITF mRNA錶達水平.結果 TBI後4、8和12d組L/M比值分彆為0.5092±0.0353,0.7174±0.0116和0.7295±0.0063,均明顯高于空白對照組(0.2908±0.0533,F=321.47,P＜0.05).TBI後4、8和12 d組ITF mRNA錶達水平分彆為0.78612±0.1428,0.2521±0.1223,和0.2306±0.0221,均顯著低于空白對照組(1.3498±0.0476,F=235.71,P＜0.05).各TBI組中L/M與腸道ITF mRNA水平呈顯著負相關(r=-0.985,P＜0.01).結論 TBI後隨著時間延長ITF mRNA錶達逐漸下降而腸黏膜通透性逐漸增加,即TBI後ITF mRNA錶達與腸道通透性呈顯著負相關.ITF在TBI所緻腸道黏膜通透性增加中起著保護作用.
목적 탐토전신조사(TBI)대장도점막통투성화장삼협인자(ITF)mRNA표체적영향이급이자지간적관계.방법 32지BALB/c소서수궤균분위4조:공백대조조화총제량8.0 Gy전신조사후4、8화12 d조.TBI제량8.0 Gy,제량솔1.0 Gy/min.고효액상색보-증발광산사검측기분석(HPLC-ELSD)검측뇨액표본중유과당여감로순적배출솔비치(L/M),평개각조소서적장도통투성;수집공장조직표본,실시형광정량PCR검측장도ITF mRNA표체수평.결과 TBI후4、8화12d조L/M비치분별위0.5092±0.0353,0.7174±0.0116화0.7295±0.0063,균명현고우공백대조조(0.2908±0.0533,F=321.47,P＜0.05).TBI후4、8화12 d조ITF mRNA표체수평분별위0.78612±0.1428,0.2521±0.1223,화0.2306±0.0221,균현저저우공백대조조(1.3498±0.0476,F=235.71,P＜0.05).각TBI조중L/M여장도ITF mRNA수평정현저부상관(r=-0.985,P＜0.01).결론 TBI후수착시간연장ITF mRNA표체축점하강이장점막통투성축점증가,즉TBI후ITF mRNA표체여장도통투성정현저부상관.ITF재TBI소치장도점막통투성증가중기착보호작용.
Objective To investigate the change of the intestinal permeability,the expression level of intestinal trefoil factor (ITF) mRNA and the relationship between them after total body irradiation (TBI),and explore the effect of TBI on the development of intestinal permeability and the expression level of ITF mRNA.Methods Twenty two BALB/c mice were randomly divided into 4 equal groups: 3 groups at 4,8 and 12 d after TBI with the total dose of 8.0 Gy and the dose rate of 1.0 Gy/min respectively,and a control group.Lactulose (L) and mannitol (M) were perfused into the esophagus before the experiment and urine samples were collected.Liquid chromatography was used to measure the L/M excretion ratio in the urine samples collected 4,8,and 12 days after the TBI.And then the mice were killed with their intestine were taken out.The expression of ITF mRNA in the jejunum tissue was detected by real-time fluorescence quantitative PCR.Results The urine L/M ratio levels of the groups 4,8 and 12 days after TBI were (0.5092 ± 0.0352),(0.7174 ± 0.0116),and (0.7295 ± 0.0533) respectively,all significantly higher than that of the control group [(0.2908 ± 0.0533),F = 321.47,P ＜ 0.05].The ITF mRNA expression levels of groups 4,8 and 12 days after TBI were (0.78612 ±0.1428),(0.2521 ±0.1223),and (0.2306 + 0.0221 ) respectively,all significantly lower than that of the control group [( 1.3498 + 0.0476),F = 235.71 ,P ＜ 0.05].The urine L/M ratio was significantly negatively correlated with the expression of ITF mRNA in all TBI groups (r = - 0.985,P ＜ 0.01 ).Conclusions The intestinal permeability increases and the expression level of ITF mRNA decreases after TBI.The urine L/M ratio is negatively correlated with the expression level of ITF mRNA after TBI.ITF is involved in protection against intestinal permeability induced by TBI.
