CAJ | 학술논문

目的探讨小凹蛋白1(CAV-1)在人皮下和网膜脂肪组织的差异表达,并分析其与肥胖及胰岛素抵抗的关系.方法 2009年1至4月在中山大学附属第三医院择期行腹部手术的正常糖调节患者41例,按体质指数(BMI)分为超重组(12例)和正常体重组(29例),采用化学发光法检测两组患者空腹胰岛素(FINS)水平,葡萄糖氧化酶法检测患者空腹血糖(FPG)水平,计算稳态模型的胰岛素抵抗指数(HOMA-IR)；分别在术中取其皮下和网膜脂肪组织,用半定量反转录(RT)-PCR和Western印迹法检测脂肪组织中CAV-1 mRNA和蛋白的表达,并对两组患者皮下和网膜脂肪组织中CAV-1 mRNA和蛋白表达水平与BMI、HOMA-IR行相关性分析.结果 (1)超重组患者血清FINS及HOMA-IR均高于正常体重组[FINS:(8.82±3.79) mU/L比(6.43±4.38)mU/L,P＜0.05；HOMA-IR:(1.91±0.85比1.36±0.72),均P＜0.05].(2)正常体重组网膜脂肪组织CAV-1mRNA的表达高于超重组(2.84±0.86比1.02±0.36,P＜0.01),但皮下脂肪组织CAV-1 mRNA的表达在两组之间差异无统计学意义(1.93±0.62比1.67±0.73,P＞0.05).(3)正常体重组网膜脂肪组织CAV-1蛋白表达明显高于超重组(1.68±0.67比0.73±0.29,P＜0.05),而在皮下脂肪组织的表达两组之间差异无统计学意义(P＞0.05).(4)网膜脂肪组织CAV-1 mRNA表达水平与BMI、腰围、甘油三酯、HOMA-IR均呈负相关(均P＜0.05).而皮下脂肪组织CAV-1 mRNA表达水平与BMI、腰围及HOMA-IR无明显相关关系(均P＞0.05).结论 CAV-1在肥胖和正常体重者的网膜脂肪组织中存在差异表达,CAV-1在网膜脂肪组织的表达下调可能参与肥胖和胰岛素抵抗的发生.
목적 탐토소요단백1(CAV-1)재인피하화망막지방조직적차이표체,병분석기여비반급이도소저항적관계.방법 2009년1지4월재중산대학부속제삼의원택기행복부수술적정상당조절환자41례,안체질지수(BMI)분위초중조(12례)화정상체중조(29례),채용화학발광법검측량조환자공복이도소(FINS)수평,포도당양화매법검측환자공복혈당(FPG)수평,계산은태모형적이도소저항지수(HOMA-IR)；분별재술중취기피하화망막지방조직,용반정량반전록(RT)-PCR화Western인적법검측지방조직중CAV-1 mRNA화단백적표체,병대량조환자피하화망막지방조직중CAV-1 mRNA화단백표체수평여BMI、HOMA-IR행상관성분석.결과 (1)초중조환자혈청FINS급HOMA-IR균고우정상체중조[FINS:(8.82±3.79) mU/L비(6.43±4.38)mU/L,P＜0.05；HOMA-IR:(1.91±0.85비1.36±0.72),균P＜0.05].(2)정상체중조망막지방조직CAV-1mRNA적표체고우초중조(2.84±0.86비1.02±0.36,P＜0.01),단피하지방조직CAV-1 mRNA적표체재량조지간차이무통계학의의(1.93±0.62비1.67±0.73,P＞0.05).(3)정상체중조망막지방조직CAV-1단백표체명현고우초중조(1.68±0.67비0.73±0.29,P＜0.05),이재피하지방조직적표체량조지간차이무통계학의의(P＞0.05).(4)망막지방조직CAV-1 mRNA표체수평여BMI、요위、감유삼지、HOMA-IR균정부상관(균P＜0.05).이피하지방조직CAV-1 mRNA표체수평여BMI、요위급HOMA-IR무명현상관관계(균P＞0.05).결론 CAV-1재비반화정상체중자적망막지방조직중존재차이표체,CAV-1재망막지방조직적표체하조가능삼여비반화이도소저항적발생.
Objective To explore the depot-specific mRNA and protein expression of caveolin-1 ( CAV-1 ) in human subcutaneous and omental adipose tissues and analyze their relationship with obesity and insulin resistance.Methods A total of 41 subjects with normal glucose regulation were recruited.Among them,there were 29 cases with normal body mass index (BMI) and 12 cases with BMI≥24 kg/m2.All were scheduled to undergone selective abdominal operations.The levels of fasting insulin (FINS) were measured by chemiluminescence enzyme-linked immunosorbent assay (ELISA) kit.Fasting plasma glucose (FPG) was tested by glucose oxidase and home model insulin resistance index (HOMA-IR) calculated.Real-time polymerase chain reaction (RT-PCR) and Western blotting were employed to assess the relative mRNA and protein expression of caveolin-1 in subcutaneous and omental adipose tissues.And the mRNA and protein expression of caveolin-1 from different fat depots were compared and their correlations with BMI and HOMA-IR analyzed.Results (1)FINS and HOMA-IR were signfificantly higher in the overweight and obesity group than those in the normal BMI group ( FINS:(8.82±3.79) mU/L vs (6.43±4.38) mU/L,P＜0.05,HOMA-1 R:1.91±0.85 vs 1.36±0.72,P＜0.05).(2)The normal BMI group patients had the higher expression levels of caveolin-1 mRNA in omental adipose tissue than ovenweight counterparts (2.84±0.86 vs 1.02±0.36,P＜0.01).But the difference in subcutaneous adipose tissue was not significant ( P＞0.05 ).(3) The caveolin-1 protein expression in omental adipose tissue of the normal BMI group was higher than that of overweight patients ( 1.68±0.67 vs 0.73±0.29,P＜0.05 ).And the difference between two groups was not significant ( P＞0.05 ).(4) The expressions of caveolin-1 mRNA in omental adipose tissue were negatively correlated with BMI,waist circumstance,triglyceride and HOMA-IR ( r=-0.441,-0.615,-0.539,-0.688,P＜0.05).No correlations were found between the expressions of caveolin-1 mRNA in subcutaneous adipose tissue with BMI,waist circumstanee and HOMA-IR ( P＞0.05 ).Conclusion Differential depot-specific expressions of caveolin-1 are present in human subcutaneous and omental adipose tissues.A low expression of caveolin-1 in omental adipose tissue may contribute to the pathogenesis of obesity and insulin resistance.