CAJ | 학술논문

目的以传统遗传重配技术选育H5N1流感病毒Vero细胞适应株,制备Vero细胞H5N1流感疫苗.方法以流感病毒Vero细胞适应株A/Yunnan/1/2005Va(H3N2)为母株与反向遗传学技术改造的禽流感病毒疫苗株A/Anhui/1/2005(H5N1)共同感染SPF鸡胚和Vero细胞,用羊抗A/Yunnan/1/2005Va(H3N2)抗体筛选,血抑试验和基因测序鉴定病毒型别,并进行重配株的其他相关生物学试验.结果获得了1株在Vero细胞高产的H5N1流感病毒,重配前后的单价灭活疫苗免疫小鼠抗体血清效价差异无统计学意义(F=0.857,P＞0.05).结论通过流感病毒Vero细胞适应株与流行株的重配和抗体筛选,可以获得H5N1流感病毒Vero细胞适应株.
목적 이전통유전중배기술선육H5N1류감병독Vero세포괄응주,제비Vero세포H5N1류감역묘.방법 이류감병독Vero세포괄응주A/Yunnan/1/2005Va(H3N2)위모주여반향유전학기술개조적금류감병독역묘주A/Anhui/1/2005(H5N1)공동감염SPF계배화Vero세포,용양항A/Yunnan/1/2005Va(H3N2)항체사선,혈억시험화기인측서감정병독형별,병진행중배주적기타상관생물학시험.결과 획득료1주재Vero세포고산적H5N1류감병독,중배전후적단개멸활역묘면역소서항체혈청효개차이무통계학의의(F=0.857,P＞0.05).결론 통과류감병독Vero세포괄응주여류행주적중배화항체사선,가이획득H5N1류감병독Vero세포괄응주.
Objective To prepare Vero cell-adapted influenza H5N1 virus strain by Genetic Reassortment and produce influenza H5N1 vaccine using Veto cell as a substrate.Methods Embryonated specific pathogen-free (SPF) hen's eggs and Veto cells were co-infected with Vero cell-adapted influenza virus A/Yunnan/1/2005 Va(H3N2) and A/Anhui/1/2005 (H5N1) via reverse genetics.The reassortant was screened with goat antibody against strain A/Yunnan/1/2005 Va(H3N2) and identified for subtype by hemagglutination-inhibition (HI) assays and gene analysis of HA and NA.Results A Vero cell-adapted influenza H5N1 virus strain was obtained,and there was no significant difference in serum antibody titers of monovalent inactivated vaccine reassorted before and after(F=0.857,P＞0.05).Conclusion The Vero cell-adapted influenza virus of epidemic strain may be reassortment between Vero cell-adapted and epidemic strains.