CAJ | 학술논문

目的针对2011年发生于云南楚雄地区的狂犬病疫情开展分子流行病学调查分析.方法通过直接免疫荧光试验(DFA)和RT-PCR方法对采集样本进行检测.采用DNAstar中MegAlign软件和MEGA 3.1软件Neighbour-joining(NJ)方法分别进行病毒样本N基因的同源性分析和系统进化分析.结果在23份脑组织或脑脊液标本中,11份实验室检测结果为阳性,阳性率为47.8％.核蛋白(N)基因同源性结果表明,来自犬只的病毒样本与基因Ⅰ型Asia 1a亚型病毒同源性最高,核苷酸同源性为99.2％～ 99.4％之间,与Asia 2c基因亚型病毒核苷酸同源性为88.5％～89.6％.进化树分析结果表明,本研究分离到的病毒样本与Yunnan-ZT07处于同一进化分支内.结论引发本次疫情的狂犬病毒属于基因Ⅰ型Asia 1a亚群,且此次疫情可能与2007年云南昭通流行毒株Yunnan-ZT07的输入关系密切.
목적 침대2011년발생우운남초웅지구적광견병역정개전분자류행병학조사분석.방법 통과직접면역형광시험(DFA)화RT-PCR방법대채집양본진행검측.채용DNAstar중MegAlign연건화MEGA 3.1연건Neighbour-joining(NJ)방법분별진행병독양본N기인적동원성분석화계통진화분석.결과 재23빈뇌조직혹뇌척액표본중,11빈실험실검측결과위양성,양성솔위47.8％.핵단백(N)기인동원성결과표명,래자견지적병독양본여기인Ⅰ형Asia 1a아형병독동원성최고,핵감산동원성위99.2％～ 99.4％지간,여Asia 2c기인아형병독핵감산동원성위88.5％～89.6％.진화수분석결과표명,본연구분리도적병독양본여Yunnan-ZT07처우동일진화분지내.결론 인발본차역정적광견병독속우기인Ⅰ형Asia 1a아군,차차차역정가능여2007년운남소통류행독주Yunnan-ZT07적수입관계밀절.
Objective To investigate the molecular epidemiologic features of rabies outbreak in Chuxiong District in Yunnan Province,China,in 2011.Methods Brain tissue specimens from dogs and domestic animals were collected and the presence of rabies virus was determined by direct immunofluorescence (DFA) and reverse transcription-polymerase chain reaction (RT-PCR) assays.Homology and phylogenetic analyses were performed using the MegAlign and MEGA3.1software,respectively.Results Eleven of 23 brain tissue specimens were confirmed positive for rabies virus by both DFA and RT-PCR assays.A nucleotide sequence homology of 99.2％ -99.4％ existed between the two nucleoprotein ( N ) gene fragments from positive specimens and the Asia 1a subgroup of genotype 1Lyssavirus.Conclusion Phylogenetic analysis indicated that the rabies viruses causing this outbreak belonged to the Asia 1a subgroup of genotype 1Lyssavirus,which is closely related to the Yunnan-ZT07 specimen isolated in Zhaotong District,Yunnan Province,in 2007.