CAJ | 학술논문

目的探讨铜绿假单胞菌密度感应系统自体诱导因子3OC12-HSL对肠道上皮细胞Caco-2凋亡的影响及其深入机制.方法 3OC12-HSL干扰Caco-2细胞4 h后,采用MTT法检测Caco-2细胞干扰前后细胞活性变化;Annexin V-FTTC/PI双染法流式细胞术检测细胞凋亡率的变化;Western blot检测Caco-2细胞中凋亡相关蛋白p-p38/MAPK及NF-κB表达变化.结果 MTT结果显示,3OC12-HSL可以抑制Caco-2细胞活性,并呈剂量依赖性(P<0.05).流式细胞术结果表明,Caco2细胞凋亡率随3OC12-HSI干扰浓度增加而增加(P<0.05).Western blot结果显示,3OC12-HSL处理后,细胞p-p38/MAPK及NF-κB蛋白表达水平增加.结论 3OC12-HSL可以抑制肠道上皮细胞Caco-2活性,诱导细胞发生凋亡,这种作用可能和p38/MAPK通路激活以及NF-κB的激活有关.
목적 탐토동록가단포균밀도감응계통자체유도인자3OC12-HSL대장도상피세포Caco-2조망적영향급기심입궤제.방법 3OC12-HSL간우Caco-2세포4 h후,채용MTT법검측Caco-2세포간우전후세포활성변화;Annexin V-FTTC/PI쌍염법류식세포술검측세포조망솔적변화;Western blot검측Caco-2세포중조망상관단백p-p38/MAPK급NF-κB표체변화.결과 MTT결과 현시,3OC12-HSL가이억제Caco-2세포활성,병정제량의뢰성(P<0.05).류식세포술결과 표명,Caco2세포조망솔수3OC12-HSI간우농도증가이증가(P<0.05).Western blot결과 현시,3OC12-HSL처리후,세포p-p38/MAPK급NF-κB단백표체수평증가.결론 3OC12-HSL가이억제장도상피세포Caco-2활성,유도세포발생조망,저충작용가능화p38/MAPK통로격활이급NF-κB적격활유관.
Objective To investigate the effect of quorum sensing autoinducer 3OC12-HSL of Pseudomonas aeruginosa on the apoptosis of Caco-2 cells and its mechanism. Methods Caco-2 cells were incubated with 3OC12-HSL for 4 h and then examined by MTT method for cytoactivities. Flow cytometry was used to analyze apoptosis rate of Caco-2 cells. Expression of apoptosis associated proteins p-p38/MAPK and NF-κB were detected by Western blot. Results After exposure to 3OC12-HSL for 4 h, cytoactivities of Caco-2 cells was reduced(P<0.05) with dose-dependent pattern, and higher dose of 3OC12-HSL leaded to increasing apoptosis rate of Caco-2 cells(P<0.05). 3OC12-HSL raised expression of apoptosis associated proteins p-p38/MAPK and NF-κB detected by Western blot. Conclusion Quorum sensing autoinducer 3OC12-HSL can effect cytoactivities of Caco-2 cells and may induce its apoptosis by enhancing the expression of p-p38/MAPK and NF-κB protein.