CAJ | 학술논문

来自嗜热脂肪土芽孢杆菌的木聚糖内切酶XT6在工业上有着重要的应用,已经成功应用于工业规模的生产试验.本文作者在合成XT6基因全序列的同时对其密码子进行了优化,且构建重组质粒在大肠杆菌中高表达.通过优化表达条件,功能正常的XT6基因在大肠杆菌中成功过量表达,蛋白表达量占细胞中总蛋白的65%.重组表达的木聚糖内切酶XT6特性和天然酶相似,以桦木木聚糖为底物测定细胞提取物中木聚糖酶活性,最大活性高达3 030 U/mL.本文首次报道了来自嗜热脂肪土芽孢杆菌中木聚糖酶基因全序列的合成和在大肠杆菌中成功过量表达.图6表1参19
래자기열지방토아포간균적목취당내절매XT6재공업상유착중요적응용,이경성공응용우공업규모적생산시험.본문작자재합성XT6기인전서렬적동시대기밀마자진행료우화,차구건중조질립재대장간균중고표체.통과우화표체조건,공능정상적XT6기인재대장간균중성공과량표체,단백표체량점세포중총단백적65%.중조표체적목취당내절매XT6특성화천연매상사,이화목목취당위저물측정세포제취물중목취당매활성,최대활성고체3 030 U/mL.본문수차보도료래자기열지방토아포간균중목취당매기인전서렬적합성화재대장간균중성공과량표체.도6표1삼19
The endoxylanase XT6 secreted from Geobacillus stearothermophilus is a particularly attractive candidate for some industrial purposes and was used successfully on an industrial-scale mill trial. The gene was de novo synthesized with the codons adjusted to fit the bias of that of Escherichia coli and constructed into vector pET28a (+). After optimizing the expression conditions, functional xylanase XT6 was over expressed in E. coll with up to 65% of total protein. A maximum xylanase activity of 3,030 U/mL was obtained from cell extract against birchwood xylan. The recombinant XT6 was partly characterized and was similar with those of the native enzyme in G. stearothermophilus. This is the first report on the over expression of a de novo synthesized xylanase XT6 gene from Geobacillus stearothermophilus. Fig 6, Tab 1, Ref 19