CAJ | 학술논문

在同标条件下比较了5种市售商品植酸酶(编号为A、B、C、D、E)的酶活力、最适pH、最适温度、酸碱耐受性、耐热性及蛋白酶耐受性,结果表明,A、B、D植酸酶的酶活力与标示值相近;A、B植酸酶在偏碱条件下具有相对高活性,C、D、E植酸酶在酸性与偏碱环境下均具有较高活力;A、B、C、D、E植酸酶最适温度在50～60℃之间;80℃热处理3 min后,A、B、E植酸酶的剩余酶活有80%以上,C、D植酸酶则仅剩余40%以下;经胃蛋白酶处理后.A、B、D、E植酸酶的剩余酶活50%以上,C植酸酶仪残余8%,而经胰蛋白酶处理后,C、D、E植酸酶剩余酶活70%以上.研究结果表明,5种商品植酸酶各有优点,但并不完全符合"理想"植酸酶的要求.
재동표조건하비교료5충시수상품식산매(편호위A、B、C、D、E)적매활력、최괄pH、최괄온도、산감내수성、내열성급단백매내수성,결과표명,A、B、D식산매적매활력여표시치상근;A、B식산매재편감조건하구유상대고활성,C、D、E식산매재산성여편감배경하균구유교고활력;A、B、C、D、E식산매최괄온도재50～60℃지간;80℃열처리3 min후,A、B、E식산매적잉여매활유80%이상,C、D식산매칙부잉여40%이하;경위단백매처리후.A、B、D、E식산매적잉여매활50%이상,C식산매의잔여8%,이경이단백매처리후,C、D、E식산매잉여매활70%이상.연구결과표명,5충상품식산매각유우점,단병불완전부합"이상"식산매적요구.
For the evaluation of the effectiveness of the commercial phytases (A, B, C, D, E) as feed additive, a series of in vitro experiments were carried out to determine the relative suitability, in vitro properties like temperature optimum, temperature stability, pH optimum and pH profile or proteolytic stability. The results showed that the enzyme activity of A, B, D had similar value; A, B in the partial alkaline conditions with relatively high activity, C, D, E in acidic and alkaline conditions were biased high vitality; the optimum temperature of A, B, C, D, E were between 50 ～ 60℃; After heating to 80℃ for 3 minutes., the residual activity of A, B, E were more than 80%, C, D only 40%; After exposure to pepsin, the phytases A , B, D, E were more than 50% residual activity, C is only 8% of the residual, and after treatment by trypsin, C, D, E were more than 70% residual activity. Five kinds of phytase goods has its own advantages, but it does not fully comply with the "ideal" requirements of phytase.