时珍国医国药
時珍國醫國藥
시진국의국약
LISHIZHEN MEDICINE AND MATERIA MEDICA RESEARCH
2009年
11期
2661-2664
,共4页
唐乾利%俞渊%黄名威%关中正%高善辉
唐乾利%俞淵%黃名威%關中正%高善輝
당건리%유연%황명위%관중정%고선휘
慢性肝损伤%肝硬化%大黄灵仙胶囊%胆结石%肝脏超微结构
慢性肝損傷%肝硬化%大黃靈仙膠囊%膽結石%肝髒超微結構
만성간손상%간경화%대황령선효낭%담결석%간장초미결구
Chronic liver injury%Cirrhosis of liver%Dahuanglinxian Capsule%Gallstones%Ultrastructure
目的 探讨大黄灵仙胶囊对慢性肝损伤家兔胆结石形成的干预作用及其作用机制.方法 将46只家兔随机分为正常组、慢性肝损伤组、肝硬化组、大黄灵仙胶囊大剂量组、大黄灵仙胶囊小剂量组.采用皮下注射CCl_4方法 进行慢性肝损伤造模,各组同时进行不同处理.最后观察各组胆囊成石率,肝脏功能生化指标,肝组织病理改变、超微结构改变,胆汁成分改变.结果 ①慢性肝损伤组、肝硬化组胆石形成阳性检出率较正常对照组显著增加(P＜0.05或P＜0.01).大黄灵仙胶囊大剂量组、大黄灵仙胶囊小剂量组胆石阳性检出率较肝硬化组有显著降低(P＜0.01或P＜0.05).②各模型组中肝脏超微结构均发生不同程度异常改变,大黄灵仙胶囊大、小剂量治疗组肝脏病理改变、肝细胞超微结构改变较模型组比较均有改善,大剂量组尤为明显.③大黄灵仙胶囊大、小剂量组血清AST,ALT,γ-GT,非结合胆红素均明显低于肝硬化组(P＜0.05或P<0.01);总胆红素、间接胆红素、钙离子大黄灵仙胶囊大、小剂量组较肝硬化组明显降低(P<0.01),大剂量组较小剂量组明显降低(P<0.01).结论 大黄灵仙胶囊对慢性肝损伤后胆结石形成具有明显的预防效果,其作用机制主要与抑制CCl_4所致肝脏损伤的作用有关,同时与大黄灵仙胶囊在多层次、多途径调节参与结石形成各因素关系密切.
目的 探討大黃靈仙膠囊對慢性肝損傷傢兔膽結石形成的榦預作用及其作用機製.方法 將46隻傢兔隨機分為正常組、慢性肝損傷組、肝硬化組、大黃靈仙膠囊大劑量組、大黃靈仙膠囊小劑量組.採用皮下註射CCl_4方法 進行慢性肝損傷造模,各組同時進行不同處理.最後觀察各組膽囊成石率,肝髒功能生化指標,肝組織病理改變、超微結構改變,膽汁成分改變.結果 ①慢性肝損傷組、肝硬化組膽石形成暘性檢齣率較正常對照組顯著增加(P＜0.05或P＜0.01).大黃靈仙膠囊大劑量組、大黃靈仙膠囊小劑量組膽石暘性檢齣率較肝硬化組有顯著降低(P＜0.01或P＜0.05).②各模型組中肝髒超微結構均髮生不同程度異常改變,大黃靈仙膠囊大、小劑量治療組肝髒病理改變、肝細胞超微結構改變較模型組比較均有改善,大劑量組尤為明顯.③大黃靈仙膠囊大、小劑量組血清AST,ALT,γ-GT,非結閤膽紅素均明顯低于肝硬化組(P＜0.05或P<0.01);總膽紅素、間接膽紅素、鈣離子大黃靈仙膠囊大、小劑量組較肝硬化組明顯降低(P<0.01),大劑量組較小劑量組明顯降低(P<0.01).結論 大黃靈仙膠囊對慢性肝損傷後膽結石形成具有明顯的預防效果,其作用機製主要與抑製CCl_4所緻肝髒損傷的作用有關,同時與大黃靈仙膠囊在多層次、多途徑調節參與結石形成各因素關繫密切.
목적 탐토대황령선효낭대만성간손상가토담결석형성적간예작용급기작용궤제.방법 장46지가토수궤분위정상조、만성간손상조、간경화조、대황령선효낭대제량조、대황령선효낭소제량조.채용피하주사CCl_4방법 진행만성간손상조모,각조동시진행불동처리.최후관찰각조담낭성석솔,간장공능생화지표,간조직병리개변、초미결구개변,담즙성분개변.결과 ①만성간손상조、간경화조담석형성양성검출솔교정상대조조현저증가(P＜0.05혹P＜0.01).대황령선효낭대제량조、대황령선효낭소제량조담석양성검출솔교간경화조유현저강저(P＜0.01혹P＜0.05).②각모형조중간장초미결구균발생불동정도이상개변,대황령선효낭대、소제량치료조간장병리개변、간세포초미결구개변교모형조비교균유개선,대제량조우위명현.③대황령선효낭대、소제량조혈청AST,ALT,γ-GT,비결합담홍소균명현저우간경화조(P＜0.05혹P<0.01);총담홍소、간접담홍소、개리자대황령선효낭대、소제량조교간경화조명현강저(P<0.01),대제량조교소제량조명현강저(P<0.01).결론 대황령선효낭대만성간손상후담결석형성구유명현적예방효과,기작용궤제주요여억제CCl_4소치간장손상적작용유관,동시여대황령선효낭재다층차、다도경조절삼여결석형성각인소관계밀절.
Objective To study the interference effect and its mechanism of Dahuanglinxian Capsules on gallstone formation after chronic liver injury of rabbits. Methods 46 rabbits were randomly divided into 5 groups, respectively named by group A ( normal control group) , group B (chronic liver injury group) , group C (liver cirrhosis group) , group D (group fed with large dose Dahuanglinxian capsule) and group E (group fed with large dose Dahuanglinxian capsule). The chronic liver injured rabbits were induced by subcutaneous injection with CC1_4 and each group was treated by different factors at the same time. The gallstone formation rate, liver function biochemical index, pathological changes in liver tissues, ultrastructural changes, and biliary component change were observed by the methods of biochemical and pathological technology. Results ①The gallstone formation rate of group B and C were obviously higher than group A (P < 0.05 or P < 0.01 ). The gallstone formation rate of D and E were obviously lower than group C (P<0.05 or P<0.01).②There were abnormal ultrastructural changes to certain extent in models B and C. Compared with the model groups, the D and E groups improved the ultrastructural changes and pathological changes in liver tissues, especially D group.③The level of AST, ALT, r-GT and indirect bilirubin in serum of groups D and E were obviously lower than group C (P<0.05 or P<0.01). At the same time, the content of total bilirubin, indirect bilirubin, Ca~(2+) of groups D and E were obviously lower than group C ( P < 0.01) , the same of group D was lower than group E (P<0.01). Conclusion The Dahuanglinxian capsules have significant effect of preventing gallstone formation due to its protective effect to liver injury which is induced by CC1_4, and its association with regulations to many factors participating in the gallstone formation on multiple levels and ways.
