安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2010年
2期
758-759,771
,共3页
铅%鲫鱼%血清%过氧化氢%髓过氧化物酶%过氧化氢酶
鉛%鯽魚%血清%過氧化氫%髓過氧化物酶%過氧化氫酶
연%즉어%혈청%과양화경%수과양화물매%과양화경매
Pb%Carassius auratus%Serum%Hydrogen peroxide%Myeloperoxidase%Catalase
[目的]研究重金属铅对鲫鱼抗氧化能力的影响.[方法]用0、0.01、0.05、0.10、0.50、1.00 mmol/L的含铅水溶液处理鲫鱼,比较不同处理鲫鱼血清过氧化氢含量、过氧化氢酶和髓过氧化物酶活力.[结果]低浓度铅(0.01 mmol/L)对鲫鱼血清CAT活力具有一定的促进作用,高浓度铅对鲫鱼血清CAT活力具有抑制作用;鲫鱼血清中H_2O_2含量随铅离子处理浓度的升高呈上升趋势,但1.00 mmol/L铅离子处理组鲫鱼血清H_2O_2含量低于0.50 mmol/L铅离子处理组;较低浓度的铅(0.01、0.05 mmol/L)能够提高鲫鱼血清MPO活力,高浓度铅则抑制鲫鱼血清MPO活力.[结论]该研究为鱼类生存环境重金属污染的定量分析提供了参考.
[目的]研究重金屬鉛對鯽魚抗氧化能力的影響.[方法]用0、0.01、0.05、0.10、0.50、1.00 mmol/L的含鉛水溶液處理鯽魚,比較不同處理鯽魚血清過氧化氫含量、過氧化氫酶和髓過氧化物酶活力.[結果]低濃度鉛(0.01 mmol/L)對鯽魚血清CAT活力具有一定的促進作用,高濃度鉛對鯽魚血清CAT活力具有抑製作用;鯽魚血清中H_2O_2含量隨鉛離子處理濃度的升高呈上升趨勢,但1.00 mmol/L鉛離子處理組鯽魚血清H_2O_2含量低于0.50 mmol/L鉛離子處理組;較低濃度的鉛(0.01、0.05 mmol/L)能夠提高鯽魚血清MPO活力,高濃度鉛則抑製鯽魚血清MPO活力.[結論]該研究為魚類生存環境重金屬汙染的定量分析提供瞭參攷.
[목적]연구중금속연대즉어항양화능력적영향.[방법]용0、0.01、0.05、0.10、0.50、1.00 mmol/L적함연수용액처리즉어,비교불동처리즉어혈청과양화경함량、과양화경매화수과양화물매활력.[결과]저농도연(0.01 mmol/L)대즉어혈청CAT활력구유일정적촉진작용,고농도연대즉어혈청CAT활력구유억제작용;즉어혈청중H_2O_2함량수연리자처리농도적승고정상승추세,단1.00 mmol/L연리자처리조즉어혈청H_2O_2함량저우0.50 mmol/L연리자처리조;교저농도적연(0.01、0.05 mmol/L)능구제고즉어혈청MPO활력,고농도연칙억제즉어혈청MPO활력.[결론]해연구위어류생존배경중금속오염적정량분석제공료삼고.
[Objective] The aim was to study the effect of Pb on antioxidant capacity of Carassius auratus.[Method] The C.auratus were treated by Pb solution with 0,0.01,0.05,0.10,0.50,1.00 mmol/L,the content of hydrogen peroxide (H_2O_2),activities of catalase (CAT) and myeloperoxidase(MPO) in serum of C.auratus in different treatments were compared.[Result] Pb with low concn.(0.01 mmol/L) could increase the activity of CAT in serum of C.auratus, while Pb with high concn.could decrease the activity of CAT.H_2O_2 content in serum of C.auratus showed increasing trend with the increase of Pb concn,but H_2O_2 content in serum of C.auratus in treatment with 1.00 mmol/L Pb was lower than that in treatment with 0.50 mmol/L Pb.Pb with low concn.(0.01,0.05 mmol/L) could increase the activity of MPO in serum of C.auratus,while Pb with high concn.could decrease the activity of MPO.[Conclusion] The study provided the reference for quantitative analysis of heavy mental pollution in living environment of fish.
