CAJ | 학술논문

目的研究低氧预适应对海马神经元和星形胶质细胞在急性缺氧暴露时的葡萄糖转运蛋白的活性和基因表达的影响.方法培养大鼠海马神经元和星形胶质细胞,每天间歇暴露于低氧混合气体(1%O_2、10%CO_2、89%N_2)20 min,连续6 d.最后1次低氧暴露24 h后,将细胞暴露于无氧混合气体(10%CO_2、90%N_2)6 h,然后立即检测[~3H]-2-脱氧葡萄糖(2-DG)的摄取率、GLUT/和GLUT3的mRNA水平及神经元的存活率.结果低氧预适应上调急性缺氧时神经元和星形胶质细胞的2-DG吸收率、星形胶质细胞GLUT1 mRNA的表达及神经元GLUT1和GLUT3 mRNA的表达,提高神经元存活率,这一作用可被细胞松弛素B消除.结论低氧预适应上调海马神经元和星形胶质细胞在急性缺氧时的葡萄糖摄取率和葡萄糖转运蛋白的基因表达.
목적 연구저양예괄응대해마신경원화성형효질세포재급성결양폭로시적포도당전운단백적활성화기인표체적영향.방법 배양대서해마신경원화성형효질세포,매천간헐폭로우저양혼합기체(1%O_2、10%CO_2、89%N_2)20 min,련속6 d.최후1차저양폭로24 h후,장세포폭로우무양혼합기체(10%CO_2、90%N_2)6 h,연후립즉검측[~3H]-2-탈양포도당(2-DG)적섭취솔、GLUT/화GLUT3적mRNA수평급신경원적존활솔.결과 저양예괄응상조급성결양시신경원화성형효질세포적2-DG흡수솔、성형효질세포GLUT1 mRNA적표체급신경원GLUT1화GLUT3 mRNA적표체,제고신경원존활솔,저일작용가피세포송이소B소제.결론 저양예괄응상조해마신경원화성형효질세포재급성결양시적포도당섭취솔화포도당전운단백적기인표체.
Objective To explore the effect of hypoxic preconditioning on the activity and gene expressions of glu-cose transporters in the cultured rat hippocampal neurons and astrocytes under anoxic condition. Methods The cultured rat hippocampal neurons and astrocytes were treated for 6 days by intermittently exposing to hypoxic gas mixture (1% O_2, 10% CO_2, 89% N_2) for20 min each day. 24 h after the last hypoxic exposure, the cells were exposed to anoxic gas mixture (10% CO_2, 90% N_2) for 6 h, and the uptake rate of [~3H]-2-deoxyglucose (2-DG), the levels of glucose transporter GLUT1 and GLUT3 mRNAs and the cell survival rate were examined im-mediately after anoxic exposure. Results Neurons and astrocytes preconditioned with hypoxia showed higher 2-DG uptake rates and increased expressions of GLUT 1 mRNA in the astrocytes and GLUT 1 and GLUT 3 mRNA in the neurons. The preconditioned neurons also showed an increased tolerance to anoxia. Conclusion Hypoxic precon-ditioning up-regulates the activity and gene expressions of glucose transporters of hippocampal neurons and astro-cytes under anoxic condition.