国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
17期
1281-1284
,共4页
慢性阻塞性肺疾病%转化生长因子-β1%Ⅰ型胶原%Ⅲ型胶原%氨茶碱
慢性阻塞性肺疾病%轉化生長因子-β1%Ⅰ型膠原%Ⅲ型膠原%氨茶堿
만성조새성폐질병%전화생장인자-β1%Ⅰ형효원%Ⅲ형효원%안다감
Chronic obstructive pulmonary disease%Transforming growth factor-β1%Type Ⅰ collagen%Type Ⅲ collagen%Aminophylline
目的 观察慢性阻塞性肺疾病( COPD)大鼠模型支气管肺组织转化生长因子-β1（TGF-β1）、Ⅰ型胶原、Ⅲ型胶原的变化以及氨茶碱药物干预的影响。方法 将35只雄性Wistar大鼠分3组；正常对照组（A组，10只）、COPD模型组（B组，15只）、氨茶碱治疗组（C组，10只）。气管内注入脂多糖(LPS)及每天烟熏法制作COPD动物模型，氨茶碱治疗组于模型制备后给予每日灌胃氨茶碱共1周，5周后对大鼠肺进行苏木素-伊红染色、Masson染色及免疫组织化学染色检测TGF-β1、Ⅰ、Ⅲ型胶原在支气管肺组织的表达。结果 ①B组支气管肺组织TGF-β1表达率为(11.75±3.14)％,较A组(2.41±1.16)％增高(P＜0.01)，C组为(5.54±2.00)％，较B组降低(P＜0.01),但仍较A组增高(P＜0.01)。②B组支气管肺组织Ⅰ型胶原表达率为（14.70±3.76）％，较A组（2.87±1.03）％增高(P＜0.01)，C组为(7.36±1.39)％，较B组降低(P＜0.01)，但仍较A组增高(P＜0.01)。B组Ⅲ型胶原表达率为(17.26±3.81)％，较A组(8.28±3.29)％增高(P＜0.01)，C组为(11.37±3.29)％，较B组降低(P＜0.01),与A组比较差异无统计学意义(P＞0.05)。③支气管肺组织TGF-β1表达率与Ⅰ型胶原、Ⅲ型胶原表达率呈显著正相关(分别为r=0.751、0.605，P＜0.01)。结论支气管肺组织胶原沉积增多是COPD气道重塑的重要病理变化。TGF-β1可能在COPD气道重塑中起重要作用。氨茶碱可以部分抑制COPD的气道重塑。
目的 觀察慢性阻塞性肺疾病( COPD)大鼠模型支氣管肺組織轉化生長因子-β1（TGF-β1）、Ⅰ型膠原、Ⅲ型膠原的變化以及氨茶堿藥物榦預的影響。方法 將35隻雄性Wistar大鼠分3組；正常對照組（A組，10隻）、COPD模型組（B組，15隻）、氨茶堿治療組（C組，10隻）。氣管內註入脂多糖(LPS)及每天煙熏法製作COPD動物模型，氨茶堿治療組于模型製備後給予每日灌胃氨茶堿共1週，5週後對大鼠肺進行囌木素-伊紅染色、Masson染色及免疫組織化學染色檢測TGF-β1、Ⅰ、Ⅲ型膠原在支氣管肺組織的錶達。結果 ①B組支氣管肺組織TGF-β1錶達率為(11.75±3.14)％,較A組(2.41±1.16)％增高(P＜0.01)，C組為(5.54±2.00)％，較B組降低(P＜0.01),但仍較A組增高(P＜0.01)。②B組支氣管肺組織Ⅰ型膠原錶達率為（14.70±3.76）％，較A組（2.87±1.03）％增高(P＜0.01)，C組為(7.36±1.39)％，較B組降低(P＜0.01)，但仍較A組增高(P＜0.01)。B組Ⅲ型膠原錶達率為(17.26±3.81)％，較A組(8.28±3.29)％增高(P＜0.01)，C組為(11.37±3.29)％，較B組降低(P＜0.01),與A組比較差異無統計學意義(P＞0.05)。③支氣管肺組織TGF-β1錶達率與Ⅰ型膠原、Ⅲ型膠原錶達率呈顯著正相關(分彆為r=0.751、0.605，P＜0.01)。結論支氣管肺組織膠原沉積增多是COPD氣道重塑的重要病理變化。TGF-β1可能在COPD氣道重塑中起重要作用。氨茶堿可以部分抑製COPD的氣道重塑。
목적 관찰만성조새성폐질병( COPD)대서모형지기관폐조직전화생장인자-β1（TGF-β1）、Ⅰ형효원、Ⅲ형효원적변화이급안다감약물간예적영향。방법 장35지웅성Wistar대서분3조；정상대조조（A조，10지）、COPD모형조（B조，15지）、안다감치료조（C조，10지）。기관내주입지다당(LPS)급매천연훈법제작COPD동물모형，안다감치료조우모형제비후급여매일관위안다감공1주，5주후대대서폐진행소목소-이홍염색、Masson염색급면역조직화학염색검측TGF-β1、Ⅰ、Ⅲ형효원재지기관폐조직적표체。결과 ①B조지기관폐조직TGF-β1표체솔위(11.75±3.14)％,교A조(2.41±1.16)％증고(P＜0.01)，C조위(5.54±2.00)％，교B조강저(P＜0.01),단잉교A조증고(P＜0.01)。②B조지기관폐조직Ⅰ형효원표체솔위（14.70±3.76）％，교A조（2.87±1.03）％증고(P＜0.01)，C조위(7.36±1.39)％，교B조강저(P＜0.01)，단잉교A조증고(P＜0.01)。B조Ⅲ형효원표체솔위(17.26±3.81)％，교A조(8.28±3.29)％증고(P＜0.01)，C조위(11.37±3.29)％，교B조강저(P＜0.01),여A조비교차이무통계학의의(P＞0.05)。③지기관폐조직TGF-β1표체솔여Ⅰ형효원、Ⅲ형효원표체솔정현저정상관(분별위r=0.751、0.605，P＜0.01)。결론지기관폐조직효원침적증다시COPD기도중소적중요병리변화。TGF-β1가능재COPD기도중소중기중요작용。안다감가이부분억제COPD적기도중소。
Objective To evaluate the expression of transforming growth factor-β1 (TGF-β1) and collagen in the bronchi and lung tissue of COPD rat models and the effects of aminophylline on them.Methods 35 Wistar rats were randomly divided into 3 groups, the healthy control group (group A), the COPD model group (group B) and aminophylline intervention group (group C). Rat COPD model was established by intratracheal instillation of lipopolysaccharide twice and daily exposure to cigarette smoke.Aminophylline intervention group received aminophylline daily for 7 days after COPD has been established. At the end of five weeks, the pathological features in the lung tussue were studied. The expressions of TGF-β1 and collagen in the bronchi and lung tissue were measured by computer image analyzer. Results The expression of TGF-β1 in bronchi and lung tissue was (11.75 ± 3.14)％ in group B,which was significantly higher than that in group A (2.41 ± 1.16)％ ( P ＜0.01 ). In group C, it was (5.54±2.00)％, which was lower than that in group B, but still higher than that in group A. The expression of type I collagen in bronchi and lung tissue was (14.70±3.76)％ in group B, which was significantly higher than that in group A (2.87 ±1.03 )％ (P ＜ 0.01 ). In group C, it was (7.36±1.39)％, which was lower than that in group B, but still higher than that in group A. The expression of type Ⅲ collagen was (17.26 ± 3.81 )％ in group B, which was significantly higher than that in group A (8.28±3.29)％ ( P ＜0.01). In group C,it was (11.37±3.29)％, which was lower than that in group B, and there was no significant difference as compared with that in group A. There were statistical positive relationships between the relative contents for the collagen and TGF-β1 in bronchi and lung tissue. Conclusions The increase depositions of collagen in the bronchi and lung tissue are important pathologic characteristics of airway remodeling in COPD. TGF-β1 may play an important role in the airway remodeling in COPD. Aminophylline may be of use in the inhibition of airway remodeling in COPD, and worth of further observation.
